Effects of different detachment procedures on viability, nitroxide reduction kinetics and plasma membrane heterogeneity of V-79 cells

Cell Biology International

Volumn 34, Issue 6, 2010, Pages 663-668

  Batista, Urška ^a   Garvas, Maja ^b   Nemec, Marjana ^b   Schara, Milan ^b   Veranič, Peter ^a   Koklic, Tilen ^b  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

^b JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

Cell detachment; Cell viability; Electron paramagnetic resonance (EPR); Radical; Spectroscopy

Indexed keywords

BUFFER; CITRIC ACID; DYE; NITROXIDE; TEMPONE; TRYPSIN; 5 DOXYLPALMITOYL 3 PHOSPHATIDYLCHOLINE; 5-DOXYLPALMITOYL-3-PHOSPHATIDYLCHOLINE; AMINE OXIDE; NITROGEN OXIDE;

ANIMAL CELL; ARTICLE; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL DAMAGE; CELL GROWTH; CELL HETEROGENEITY; CELL MEMBRANE FLUIDITY; CELL MEMBRANE PERMEABILITY; CELL MEMBRANE TRANSPORT; CELL METABOLISM; CELL SEPARATION; CELL STRAIN V 79; CELL SUSPENSION; CELL VIABILITY; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; LIPOPHILICITY; MEMBRANE STRUCTURE; NONHUMAN; REDUCTION KINETICS; SCANNING ELECTRON MICROSCOPY; ANIMAL; CELL LINE; CELL MEMBRANE; CELL SURVIVAL; CHEMISTRY; CULTURE TECHNIQUE; HAMSTER; KINETICS; MECHANICAL STRESS; MEMBRANE FLUIDITY; METABOLISM; PHYSIOLOGY; SPIN LABELING;

ANIMALS; CELL CULTURE TECHNIQUES; CELL LINE; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; CITRATES; CRICETINAE; CYCLIC N-OXIDES; ELECTRON SPIN RESONANCE SPECTROSCOPY; KINETICS; MEMBRANE FLUIDITY; MICROSCOPY, ELECTRON, SCANNING; NITROGEN OXIDES; SPIN LABELS; STRESS, MECHANICAL; TRIACETONEAMINE-N-OXYL; TRYPSIN;

EID: 77955059046     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20090276     Document Type: Article

References (31)

1. Arsov Z, Schara M, Strancar J. Quantifying the lateral lipid domain properties in erythrocyte ghost membranes using EPR-spectra decomposition. J Magn Reson 2002;157:52-60.
2. Arsov Z, Schara M, Zorko M, Strancar J. The membrane lateral domain approach in the studies of lipid-protein interaction of GPI-anchored bovine erythrocyte acetylcholinesterase. Eur Biophys J 2004;33:715-25.
3. Batista U, Sentjurc M. EPR study of the sea anemone cytolysin, equinatoxin II, cytotoxicity on V-79 cells. Cell Biol Int 1995;19:215-22.
4. Belagyi J, Pas M, Raspor P, Pesti M, Pali T. Effect of hexavalent chromium on eukaryotic plasma membrane studied by EPR spectroscopy. Biochim Biophys Acta 1999;1421:175-82.
5. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
6. Chen K, Morse PD, Swartz HM. Kinetics of enzyme-mediated reduction of lipid soluble nitroxide spin labels by living cells. Biochim Biophy Acta 1988;943:477-84.
7. Delhotal B, Lemonnier F, Couturier M, Lemonnier A. Influence of two cell harvesting methods on intracellular ATP and amino acid concentrations in human fibroblast cultures. Biochimie 1983;65:121-5.
8. Freeman BA, Rosen GM, Barber MJ. Superoxide perturbation of the organization of vascular endothelial cell membranes. J Biol Chem 1986;261:6590-3.
9. Hajela K, Pande AH, Sumati. Carbohydrate induced modulation of cell membrane. VI. Binding of exogenous lectin induces susceptibility of erythrocytes to free radical damage: a spin label study. FEBS Lett 1997;406:255-8.
10. Heng BC, Liu H, Ge Z, Cao T. Mechanical dissociation of human embryonic stem cell colonies by manual scraping after collagenase treatment is much more detrimental to cellular viability than is trypsinization with gentle pipetting. Biotechnol Appl Biochem 2007;47:33-7.
11. Henzel K, Thorborg C, Hofmann M, Zimmer G, Leuschner U. Toxicity of ethanol and acetaldehyde in hepatocytes treated with ursodeoxycholic or tauroursodeoxycholic acid. Biochim Biophys Acta 2004;1644:37-45.
12. Huggins JW, Chesnut RW, Durham NN, Carraway K. Molecular changes in cell surface membranes resulting from trypsinization of sarcoma 180 tumor cells. Biochim Biophys Acta 1976;426:630-7.
13. Koklic T, Pirs M, Zeisig R, Abramovic Z, Sentjurc M. Membrane switch hypothesis. 1. Cell density influences lateral domain structure of tumor cell membranes. J Chem Inf Model 2005;45:1701-7.
14. Koklic T, Zeisig R, Sentjurc M. Interaction of alkylphospholipid liposomes with MT-3 breast-cancer cells depends critically on cholesterol concentration. Biochim Biophys Acta 2008;1778:2682-9.
15. Kramaric P, Pavlica Z, Koklic T, Nemec A, Erzen NK, Sentjurc M. Membrane switch hypothesis. 2. Domain structure of phagocytes in horses with recurrent airway obstruction. J Chem Inf Model 2005;45:1708-15.
16. Lord-Fontaine S, Averill DA. Enhancement of cytotoxicity of hydrogen peroxide by hyperthermia in Chinese hamster ovary cells: role of antioxidant defenses. Arch Biochem Biophys 1999;363:283-95.
17. Phillips HJ. Dye exclusion tests for cell viability. In Kruse PF and Patterson MK, editors. Tissue culture - methods and application. New York-London: Academic Press; 1973. pp. 406-8.
18. Samuni Y, Gamson J, Samuni A, Yamada K, Russo A, Krishna MC, Mitchell JB. Factors influencing nitroxide reduction and cytotoxicity in vitro. Antioxid Redox Signal 2004;6:587-95.
19. Sentjurc M, Stalc A, Suput D. Influence of equinatoxin II on coronary smooth muscle membrane fluidity. Pflugers Arch 1996;431:R317-8.
20. Sentjurc M, Zorec M, Cemazar M, Auersperg M, Sersa G. Effect of vinblastine on cell membrane fluidity in vinblastine-sensitive and -resistant HeLa cells. Cancer Lett 1998;130:183-90.
21. Sok M, Sentjurc M, Schara M. Membrane fluidity characteristics of human lung cancer. Cancer Lett 1999;139:215-20.
22. Stalc A, Sentjurc M, Sersa G, Novakovic S. The influence of TNF on the membrane fluidity of tumor cells. Cancer Lett 1992;65:183-7.
23. Strancar J, Sentjurc M, Schara M. Fast and accurate characterization of biological membranes by EPR spectral simulations of nitroxides. J Magn Reson 2000;142:254-65.
24. Strancar J, Schara M, Pecar S. New EPR method for cellular surface characterization. J Membr Biol 2003;193:15-22.
25. Subczynski WK, Lomnicka M, Hyde JS. Permeability of nitric oxide through lipid bilayer membranes. Free Radical Res 1996;24:343-9.
26. Swartz HM, Sentjurc M, Morse PD. Cellular-metabolism of water-soluble nitroxides - effect on rate of reduction of cell nitroxide ratio, oxygen concentrations and permeability of nitroxides. Biochim Biophys Acta 1986;888:82-90.
27. Todd AP, Mehlhorn RJ, Macey RI. Amine and carboxylate spin probe permeability in red cells. J Membr Biol 1989;109:41-52.
28. Tsang SH, Kan LS. 300 MHz proton NMR study of the differentiation of diploid human epidermal keratinocytes. Cell Biophys 1990;16:127-38.
29. Yoon SJ. Role of citrate toxicity on cardiac excitation-contraction coupling mechanism. Master of Science Thesis, University of Toronto, 1996.
30. Zeisig R, Koklic T, Wiesner B, Fichtner I, Sentjurc M. Increase in fluidity in the membrane of MT3 breast cancer cells correlates with enhanced cell adhesion in vitro and increased lung metastasis in NOD/SCID mice. Arch Biochem Biophys 2007;459:98-106.
31. Zuvic-Butorac M, Batista U, Schara M. EPR spectra of cell membranes: a comparative study of erythrocytes and fibroblasts. Periodicum Biologorum 2001;103:235-40.