Multiplexed resequencing analysis to identify rare variants in pooled DNA with barcode indexing using next-generation sequencer

Journal of Human Genetics

Volumn 55, Issue 7, 2010, Pages 448-455

  Mitsui, Jun ^a   Fukuda, Yoko ^a   Azuma, Kyo ^b   Tozaki, Hirokazu ^b   Ishiura, Hiroyuki ^a   Takahashi, Yuji ^a   Goto, Jun ^a   Tsuji, Shoji ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b Life Technologies Japan Ltd   (Japan)

Author keywords

barcode indexing; glucocerebrosidase gene (GBA); multiple rare variants; next generation sequencer; pooled DNA; SOLiD System

Indexed keywords

GENOMIC DNA; GLUCOSYLCERAMIDASE;

ANALYTICAL ERROR; ANALYZER; ARTICLE; DNA BARCODING; DNA SEQUENCE; FALSE POSITIVE RESULT; GENETIC VARIABILITY; HUMAN; INFORMATION PROCESSING; MULTIPLEX POLYMERASE CHAIN REACTION; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; AUTOMATIC DATA PROCESSING; BASE SEQUENCE; BIAS (EPIDEMIOLOGY); DNA; GENE LIBRARY; GENETIC VARIATION; HUMANS; POINT MUTATION; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 77954972674     PISSN: 14345161     EISSN: None     Source Type: Journal    
DOI: 10.1038/jhg.2010.46     Document Type: Article

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