The mechanical binding strengths of Helicobacter pylori BabA and SabA adhesins using an adhesion binding assay-ELISA, and its clinical relevance in Japan

Microbiology and Immunology

Volumn 54, Issue 8, 2010, Pages 442-451

  Nishioka, Mitsuaki ^a   Takeuchi, Hiroaki ^a   Con, Sergio A ^a   Uehara, Yoshio ^a   Nishimori, Isao ^a   Okumiya, Toshika ^b   Kumon, Yoshitaka ^a   Sugiura, Tetsuro ^a  

^a KOCHI MEDICAL SCHOOL   (Japan)

^b KUMAMOTO UNIVERSITY   (Japan)

Author keywords

Clinical relevance; Functional adhesion; Helicobacter pylori adhesins (BabA and SabA); In house ABA ELISA

Indexed keywords

ADHESIN; PROTEIN BABA; PROTEIN SABA; UNCLASSIFIED DRUG; BABA PROTEIN, HELICOBACTER PYLORI; PROTEIN BINDING; SABA PROTEIN, HELICOBACTER PYLORI;

AGED; ARTICLE; BACTERIUM ISOLATE; BINDING AFFINITY; BINDING ASSAY; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE SEQUENCE; HELICOBACTER PYLORI; HUMAN; JAPANESE; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN FUNCTION; SEQUENCE ANALYSIS; STOMACH ADENOCARCINOMA; AMINO ACID SEQUENCE; BACTERIUM ADHERENCE; CHEMISTRY; CLASSIFICATION; GENETICS; HELICOBACTER INFECTION; JAPAN; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PHYLOGENY; PHYSIOLOGY; SEQUENCE ALIGNMENT; STOMACH TUMOR;

ADHESINS, BACTERIAL; AMINO ACID SEQUENCE; BACTERIAL ADHESION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; JAPAN; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROTEIN BINDING; SEQUENCE ALIGNMENT; STOMACH NEOPLASMS;

EID: 77954966370     PISSN: 03855600     EISSN: 13480421     Source Type: Journal    
DOI: 10.1111/j.1348-0421.2010.00237.x     Document Type: Article

References (33)

1. Rothenbacher D., Inceoglu J., Bode G., Brenner H. (2000) Acquisition of Helicobacter pylori infection in a high-risk population occurs within the first 2 years of life. J Pediatr 136: 744-8.
2. Uemura N., Okamoto S., Yamamoto S., Matsumura N., Yamaguchi S., Yamakido M., Taniyama K., Sasaki N., Schlemper R.J. (2001) Helicobacter pylori infection and the development ofgastric cancer. N Engl J Med 345: 784-9.
3. Parsonnet J., Hansen S., Rodriguez L., Gelb A.B., Warnke R.A., Jellum F., Orentreich N., Vogelman J.H., Friedman G.D. (1994) Helicobacter pylori infection and gastric lymphoma. N Engl J Med 330: 1267-1271.
4. Ilver D., Arnqvist A., Ogren J., Frick I.M., Kersulyte D., Incecik E.T., Berg D.E., Covacci A., Engstrand L., Boren T. (1998) Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science 279: 373-7.
5. Mahdavi J., Sonden B., Hurtig M., Olfat F.O., Forsberg L., Roche N., Angstrom J., Larsson T., Teneberg S., Karlsson K.A., Altraja S., Wadstrom T., Kersulyte D., Berg D.E., Dubois A., Petersson C., Magnusson K.E., Norberg T., Lindh F., Lundskog B.B., Arnqvist A., Hammarstrom L., Boren T. (2002) Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation. Science 297: 573-8.
6. Odenbreit S., Till M., Hofreuter D., Faller G., Haas R. (1999) Genetic and functional characterization of the alpAB gene locus essential for adhesion of Helicobacter pylori. Mol Microbiol 31: 1537-48.
7. Peck B., Ortkamp M., Diehl K.D., Hundt E., Knapp B. (1999) Conservation, localization and expression of HopZ, a protein involved in adhesion of Helicobacter pylori. Nucleic Acids Res 27: 3325-33.
8. Boren T., Falk P., Roth K.A., Larson G., Normark S. (1993) Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens. Science 262: 1892-5.
9. de Jonge R., Pot R.G., Loffeld R.J., van Vliet A.H., Kuipers E.J., Kusters J.G. (2004) The functional status of the Helicobacter pylori sabB adhesin gene as a putative marker for disease outcome. Helicobacter 9: 158-64.
10. Gatti L.L., Fagundes E., Souza, cagA vacA alelles and babA2 genotypes of Helicobacter pylori associated with gastric disease in Brazilian adult patients. Diagn Microbiol Infect Dis 51:231-5.
11. Gerhard M., Lehn N., Neumayer N., Boren T., Rad R., Schepp W., Miehlke S., Classen M., Prinz C. (1996) Clinical relevance ofthe Helicobacter pylori gene for blood-group antigen-binding adhesin. Proc Natl Acad Sci USA 96:12778-83.
12. Mattar R., dos Santos A.F., Eisig J.N., Rodrigues T.N., Silva F.M., Lupinacci R.M., Iriya K., Carrilho F.J. (2005) No correlation of babA2 with vacA and cagA genotype of Helicobacter pylori and grading of gastritis from peptic ulcer disease patients in Brazil. Helicobacter 10: 601-8.
13. Podzorski R.P., Podzorski D.S., Wuerth A., Tolia V. (2003) Analysis of the vacA, cagA, cagE, iceA, and babA2 genes in Helicobacter pylori from sixty-one pediatric patients from the Midwestern United States. Diagn Microbiol Infect Dis 46: 83-8.
14. Zambon C.F., Navaglia F., Basso D., Rugge M., Plebani M. (2003) Helicobacter pylori babA2, cagA, and s1 vacA genes work synergistically in causing intestinal metaplasia. J Clin Pathol 56: 287-291.
15. Mizushima T., Sugiyama T., Komatsu Y., Ishizuka J., Kato M., Asaka M. (2001) Clinical relevance of babA2 genotype of Helicobacter pylori in Japanese clinical isolates. J Clin Microbiol 39: 2463-5.
16. Sheu B.S., Sheu S.M., Yang H.B., Huang A.H., Wu J.J. (2003) Host gastric Lewis expression determines the bacterial density of Helicobacter pylori in babA2 genopositive infection. Gut 52: 927-32.
17. Yanai A., Maeda S., Hikiba Y., Shibata W., Ohmae T., Hirata Y., Ogura K., Yoshida H., Omata M. (2007) Clinical relevance of Helicobacter pylori sabA genotype in Japanese clinical isolates. J Gastroenterol Hepatol 22: 2228-32.
18. Aspholm-Hurtig M., Dailide G., Lahmann M., Kalia A., Ilver D., Roche N., Vikstrom S., Sjostrom R., Linden S., Backstrom A., Lundberg C., Arnqvist A., Mahdavi J., Nilsson U.J., Velapatino B., Gilman R.H., Gerhard M., Alarcon T., Lopez-Brea M., Nakazawa T., Fox J.G., Correa P., Dominguez-Bello M.G., Perez-Perez G.I., Blaser M.J., Normark S., Carlstedt I., Oscarson S., Teneberg S., Berg D.E., Boren T. (2004) Functional adaptation of BabA, the H. pylori ABO blood group antigen binding adhesin. Science 305: 519-22.
19. Fujimoto S., Olaniyi Ojo O., Arnqvist A., Yih Wu J., Odenbreit S., Haas R., Graham D.Y., Yamaoka Y. (2007) Helicobacter pylori BabA expression, gastric mucosal injury, and clinical outcome. Clin Gastroenterol Hepatol 5: 49-58.
20. Takeuchi H., Shirai M., Akada J.K., Tsuda M., Nakazawa T. (1998) Nucleotide sequence and characterization of cdrA, a cell division-related gene of Helicobacter pylori. J Bacteriol 180: 5263-8.
21. Takeuchi H., Nakazawa T., Okamoto T., Shirai M., Kimoto M., Nishioka M., Con S.A., Morimoto N., Sugiura T. (2006) Cell elongation and cell death of Helicobacter pylori is modulated by the disruption of cdrA (cell division-related gene A). Microbiol Immunol 50: 487-97.
22. Walz A., Odenbreit S., Mahdavi J., Boren T., Ruhl S. (2005) Identification and characterization of binding properties of Helicobacter pylori by glycoconjugate arrays. Glycobiology 15: 700-8.
23. Olfat F.O., Zheng Q., Oleastro M., Voland P., Boren T., Karttunen R., Engstrand L., Rad R., Prinz C., Gerhard M. (2005) Correlation of the Helicobacter pylori adherence factor BabA with duodenal ulcer disease in four European countries. FEMS Immunol Med Microbiol 44: 151-6.
24. Pride D.T., Meinersmann R.J., Blaser M.J. (2001) Allelic variation within Helicobacter pylori babA and babB. Infect Immun 69: 1160-71.
25. Kumar S., Tamura K., Nei M. (2004) MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5: 150-63.
26. Hennig E.E., Mernaugh R., Edl J., Cao P., Cover T.L. (2004) Heterogeneity among Helicobacter pylori strains in expression of the outer membrane protein BabA. Infect Immun 72: 3429-35.
27. Unemo M., Aspholm-Hurtig M., Iliver D., Bergstrom J., Boren T., Danielsson D., Teneberg S. (2005) The sialic acid binding SabA adhesin of Helicobacter pylori is essential for nonopsonic activation of human neutrophils. J Biol Chem 280:15390-7.
28. Petersson C., Forsberg M., Aspholm M., Olfat F.O., Forslund T., Boren T., Magnusson K.E. (2006) Helicobacter pylori SabA adhesin evokes a strong inflammatory response in human neutrophils which is down-regulated by the neutrophil-activating protein. Med Microbiol Immunol 195: 195-206.
29. Aspholm M., Olfat F.O., Norden J., Sonden B., Lundberg C., Sjostrom R., Altraja S., Odenbreit S., Haas R., Wadstrom T., Engstrand L., Semino-Mora C., Liu H., Dubois A., Teneberg S., Arnqvist A., Boren T. (2006) SabA is the H. pylori hemagglutinin and is polymorphic in binding to sialylated glycans. PLo S Pathogens 2: e110.
30. Sheu B.S., Odenbreit S., Hung K.H., Liu C.P., Sheu S.M., Yang H.B., Wu J.J. (2006) Interaction between host gastric sialyl-Lewis X and H. pylori SabA enhances H. pylori density in patients lacking gastric Lewis b antigen. Am J Gastroenterol 101:36-44.
31. Evans DG, Karjalainen TK, Evans DJ Jr, Graham DY, Lee CH. (1993) Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori. J Bacteriol 175: 674-683.
32. Alkout A.M., Blackwell C.C., Weir D.M., Poxton I.R., Elton R.A., Luman W., Palmer K. (1997) Isolation of a cell surface component of Helicobacter pylori that binds H type 2, Lewis(a), and Lewis(b) antigens. Gastroenterology 112: 1179-1187.
33. Styer C.M., Hansen L.M., Cooke C.L., Gundersen A.M., Choi S.S., Berg D.E., Benghezal M., Marshall B.J., Peek R.M. Jr, Boren T., Solnick J.V. (2010) Expression of the BabA adhesin during experimental infection with Helicobacter pylori. Infect Immun 78: 1593-1600.