Identification of CHO endogenous gene regulatory elements

Molecular Biotechnology

Volumn 45, Issue 3, 2010, Pages 235-240

  Pontiller, Jens ^a   Maccani, Andreas ^a   Baumann, Martina ^a   Klancnik, Ingo ^b   Ernst, Wolfgang ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b POLYMUN SCIENTIFIC IMMUNBIOLOGISCHE FORSCHUNG GMBH   (Austria)

Author keywords

Chinese hamster ovary cells; DNA fragmentation; Endogenous promoter; Library construction; Recombinant protein expression

Indexed keywords

CHINESE HAMSTER OVARY CELLS; DNA FRAGMENTATION; ENDOGENOUS PROMOTER; ENDOGENOUS PROMOTERS; RECOMBINANT PROTEIN EXPRESSION;

AMPLIFICATION; CELL CULTURE; CELL PROLIFERATION; DNA; GENE EXPRESSION; GENETIC ENGINEERING; MAMMALS; PLANTS (BOTANY); PROTEINS;

GENE EXPRESSION REGULATION;

GENOMIC DNA;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; ARTICLE; CELL CYCLE; CELL HETEROGENEITY; CELL POPULATION; CELL STRESS; CONTROLLED STUDY; GENE AMPLIFICATION; GENE EXPRESSION; GENE LIBRARY; GENE OVEREXPRESSION; GENE REGULATORY NETWORK; GENE SEQUENCE; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN BINDING;

ANIMALS; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; ELECTROPHORESIS, AGAR GEL; GENE LIBRARY; GENES, REPORTER; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; REGULATORY ELEMENTS, TRANSCRIPTIONAL;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77954957580     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9278-1     Document Type: Article

References (15)

1. Werner, R., Noé, W., Kopp, K., & Schlüter, M. (1998). Appropriate mammalian expression systems for biopharmaceuticals. Arzneimittelforschung, 48, 870-880.
2. Spenger, A., Ernst, W., Condreay, J., Kost, T., & Grabherr, R. (2004). Influence of promoter choice and trichostatin A treatment on expression of baculovirus delivered genes in mammalian cells. Protein Expression and Purification, 38, 17-23.
3. Dale, L. (2006). Mammalian expression cassette engineering for high-level protein production. BioProcess International, 4, 14-22.
4. Sambrook, J., & Russel, D. W. (2001). Molecular cloning, a laboratory manual (3rd ed.). Cold Spring Habor, NY: Cold Spring Harbor Laboratory Press.
5. Davison, P. (1959). The effect of hydrodynamic shears on the deoxyribonucleic acid from T2 and T4 bacteriophages. Proceedings of the National Academy of Sciences USA, 45, 1560-1568.
6. Davison, P. (1960). Sedimentation of deoxyribonucleic acid isolated under low hydrodynamic shear. Nature, 185, 918-920.
7. Schriefer, L., Gebauer, B., Qui, L., Waterston, R., & Wilson, R. (1990). Low pressure DNA shearing: A method for random DNA sequence analysis. Nucleic Acids Research, 18, 7455-7456.
8. Okpodu, C., Robertson, D., Boss, W., Togasaki, R., & Surzycki, S. (1994). Rapid isolation of nuclei from carrot suspension culture cells using a BioNebulizer. Biotechniques, 16, 154-159. (Pubitemid 2033241)
9. Deininger, P. (1983). Random subcloning of sonicated DNA: Application to shotgun DNA sequence analysis. Analytical Biochemistry, 129, 216-223.
10. Hershey, A. D. (1960). Molecular homogeneity of the deoxyribonucleic acid of phage T2. BioProcess International, 2, 143-152.
11. Anderson, S. (1981). Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Research, 9, 3015-3027.
12. Sandelin, A., Carninci, P., Lenhard, B., Ponjavic, J., Hayashizaki, Y., & Hume, D. (2007). Mammalian RNA polymerase II core promoters: Insights from genome-wide studies. Nature Reviews Genetics, 8, 424-436.
13. Levine, M., & Tjian, R. (2003). Transcription regulation and animal diversity. Nature, 424, 147-151.
14. Consortium for Chinese Hamster Ovary Cell Genomics (2009) http://hugroup.cems.umn.edu/CHO/cho-index.html.
15. Ochman, H., Gerber, A., & Hartl, D. (1988). Genetic applications of an inverse polymerase chain reaction. Genetics, 120, 621-623.