Measurement of sperm DNA integrity by sperm chromatin dispersion test and TdT-mediated dUTP nick end labeling assay

National Medical Journal of China

Volumn 89, Issue 14, 2009, Pages 970-972

  Zhang, Li Hong ^a   Qiu, Yi ^a   Wang, Ke Hua ^a   Li, Juan ^a   Zhang, Mei Xin ^a   Liu, Jing ^a   Jia, Yi Fang ^a   Wu, Ai Hua ^a   Zhang, Ai Dong ^a   Wang, Lei Guang ^a  

^a Shandong Institute for Family Planning   (China)

Author keywords

DNA damage; In situ nick end labeling; Infertility, male; Spermatozoa

Indexed keywords

DNA;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSIS, MEASUREMENT AND ANALYSIS; DNA DAMAGE; DNA FRAGMENTATION; HUMAN; INFERTILITY; MALE; NICK END LABELING; SPERM; SPERM CHROMATIN DISPERSION TEST; SPERMATOZOON MOTILITY;

ADULT; CASE-CONTROL STUDIES; DNA; DNA DAMAGE; DNA FRAGMENTATION; HUMANS; INFERTILITY, MALE; MALE; SPERM MOTILITY; SPERMATOZOA;

EID: 77954934670     PISSN: 03762491     EISSN: None     Source Type: Journal    
DOI: 10.3760/cma.j.issn.0376-2491.2009.14.010     Document Type: Article

References (13)

1. Agarwal A, Allamaneni SS. Sperm DNA damage assessment: a test whose time has come. Fertil Steril, 2005,84:850-853. (Pubitemid 41414570)
2. Tateyama H, Tada T, Hattori H, et al. Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA. Arch Pathol Lab Med, 1998,122:252-255. (Pubitemid 28115270)
3. Fernández JL, Muriel L, Goyanes V, et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril, 2005,84:833-842. (Pubitemid 41414567)
4. Younglai EV, Holt D, Brown P, et al. Sperm swim-up techniques and DNA fragmentation. Hum Reprod, 2001,16:1950-1953.
5. Fernández JL, Muriel L. Rivero MT, et al. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J Androl, 2003,24:59-66. (Pubitemid 44698494)
6. Erenpreiss J, Jepson K, Giwercman A, et al. Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays. Hum Reprod, 2004,19:2277-2282. (Pubitemid 39406688)
7. Chohan KR, Griffin JT, Lafromboise M, et al. Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J Androl, 2006,27:53-59. (Pubitemid 43106734)
8. Gunalp S, Onculoglu C. Curgan T, et al. A study of semen parameters with emphasis on sperm morphology in a fertile population: an attempt to develop clinical thresholds. Hum Reprod, 2001,16:110-114. (Pubitemid 32058590)
9. Greco E, Iacobelli M, Rienzi L, et al. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl, 2005,26:349-353. (Pubitemid 40628971)
10. Greco E, Romano S, Iacobelli M. et al. ICSI in cases of sperm DNA damage: beneficial effect of oral antioxidant treatment. Hum Reprod, 2005,20:2590-2594. (Pubitemid 41222514)