Analysis of direct and indirect methods for somatic cell counts in the milk of healthy goats;Análise das metodologias diretas e indiretas para a contagem de células somáticas no leite de cabras hígidas

Pesquisa Veterinaria Brasileira

Volumn 30, Issue 4, 2010, Pages 311-316

  Madureira, Karina Medici ^a   Gomes, Viviani ^a   de Castro, Roberto Soares ^b   Kitamura, Sandra Satiko ^c   de Araújo, Wanderley Pereira ^d  

^a Prog de Mestrado Profissional em Farmacia e Prog de Mestrado e Doutorado em Biotecnologia e Inovacao em Saude   (Brazil)

^b UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO   (Brazil)

^c ANHEMBI MORUMBI UNIVERSITY   (Brazil)

^d UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Flow cytometry; Goats.; Green pyronine Y stain

Indexed keywords

EID: 77954869260     PISSN: 0100736X     EISSN: 16785150     Source Type: Journal    
DOI: 10.1590/s0100-736x2010000400005     Document Type: Article

References (35)

1. Andrade P.V.P., Souza M.R., Borges I. & Penna C.F.A.M. 2001. Contagem de células somáticas em leite de cabra. Arq. Bras. Med. Vet. Zootec. 53(3). Disponível em . Acesso em 25 ago. 2003.
2. Arcuri E.F., Silva P.D.L., Brito J.R.F., Silva M.R. & Souza G.N. 2004. Emprego do Somacount 300, calibrado com leite de vaca, na contagem de células somáticas no leite de cabra. Ciência Rural 34(5):1497-1500.
3. Contreras A., Sierra D., Corrales J.C., Sánchez A. & Marco J. 1996. Physiological threshold of somatic cell count and California Mastitis Test for diagnosis of caprine subclinical mastitis. Small Rumin. Res. 21:259-264.
4. Droke E.A., Paape M.J. & DiCarlo A.L. 1993. Prevalence of high somatic cell counts in bulk tank goat milk. J. Dairy Sci. 76(4):1035-1039.
5. Dulin A.M., Paape M.J., Schultze W.D. & Weinland B.T. 1983. Effect of parity stage of lactation, and intramamary infection. J. Dairy Sci. 66(11): 2426-2433.
6. Dulin A.M., Paape M.J. & Weinland B.T. 1982. Cytospin centrifuge in differencial counts of milk somatic cells. J. Dairy Sci. 65:1247-1251.
7. Fernandes M.A. 2002. Avaliação das características físico-químicas, celulares e microbiológicas do leite de cabras Saanen e Alpina, criadas no Estado de São Paulo. Tese de Doutorado, USP, São Paulo. 152p.
8. Gomes V. 2003. Influência do estágio de lactação na secreção láctea de cabras (Capra hircus) Saanen. Dissertação de Mestrado, USP, São Paulo. 86p.
9. nd Seminar on Production and Utilization of Ewe and Goat Milk, Crete. Int. Dairy Fed., Brussels.
10. a ed. Guanabara Koogan, Rio de Janeiro. 419p.
11. Guidry A.J., Paape M.J. & Pearson R.E. 1976. Effects of parturition and lactation on blood and milk cell concentrations, corticosteroids, and neutrophil phagocytosis in the cow. Am. J. Vet. Res. 37(10):1195-1200.
12. Haenlein G.F.W. & Krauss W.C. 1974. Effects of single injections of diethylstilbestrol on milk composition and count leucocytes in milk Holstein-Friesian cattle. Z. Tierphysiol. Tierernährung 34:50-60.
13. Hinckley L.S. 1983. Somatic cell count in relation to caprine mastitis. Vet. Med. Small Anim. Clin. 78(8):1267-1271.
14. Lewter M.M., Mullowney P.C., Baldwin E.W. & Walker R.D. 1984. Mastitis in goat. Compend. Cont. Educ. 6(7):417-425.
15. McDougall S. & Voermans M. 2002. Influence of estrus on somatic cell count in dairy goats. J. Dairy Sci. 85(2):378-383.
16. Paape M.J., Hafs H.D. & Snyder W.W. 1963. Variations of estimated numbers of milk somatic cells staines with Wright's stains or Pyronin Y-methil green stain. J. Dairy Sci. 46(11):1211-1216.
17. Paape M.J. & Capuco A.V. 1996. Cellular defense mechanisms in the udder and lactation of goats. J. Anim. Sci. 75(2):565.
18. Paape M.J., Poutrel B., Contreras A., Marco J.C. & Capuco A.V. 2001. Milk somatic cells and lactation in small ruminants. J. Dairy Sci. 84(Suppl.E):237-244.
19. Park Y.W. & Humphrey R.D. 1986. Bacterial cell counts in goat milk and their correlations with somatic cell counts, percent fat, and protein. J. Dairy Sci. 69:32-37.
20. Perrin G.G. & Baudry C. 1993. Numérations cellulaires du lait de chevre. Le Lait 73(5/6):489-497.
21. Pettersen K. 1981. Cell content in goats milk. Acta Vet. Scand. 22:226-237.
22. Poutrel B. & Lerondelle C. 1983. Cell content of goat milk: California mastitis test, coulter counter, and fossomatic for predicting half infection. J. Dairy Sci. 66(12):2575-2576.
23. Prescott S.C. & Breed R.S. 1910. The determination of the number of the body cells in milk by a direct method. J. Infect. Dis. 7:632-640.
24. Rota A.M., Rojas A., Martin L., Rodriguez P. & Tovar J.J. 1994. Uso de la prueba de California para la deteccion de mamits en el ganado caprino. Avances en Alim. y Mej. Anim. 2(34):67-69.
25. Sampaio I.B. 1998. Estatística Aplicada à Experimentação Animal. Universidade Federal de Minas Gerais, Belo Horizonte. 221p.
26. SAS Institute 2001. SAS User's Guide: Statistics. Cary. 956p.
27. Schalm O.W. & Noorlander D.O. 1957. Experiments and observations leading to developments and the California Mastitis Test. J. Am. Vet. Med. Assoc. 130(5):199-207.
28. Schalm O.W., Carrol E.J. & Jain N.C. 1971. Bovine mastitis: Physical and chemical tests for detection of mastitis. Lea and Febiger, Philadelphia, p.128-157.
29. th ed. Iowa State University Press, Ames. 593p.
30. th International Symposium on the Milking of Small Ruminants, 1999, Athens, Wageningen.
31. Silva E.R. 1997. Estudo de algumas fontes de variação do conteúdo celular do leite de cabra. Dissertação de Mestrado, Universidade Federal Rural de Pernambuco, Recife. 62p.
32. Silva E.R., Saukas T.N., Alves S.F.A. & Pinheiro R.R. 1996. Contagem de células somáticas e California Mastitis Test no diagnóstico da mastite caprina subclínica. Revta Bras. Med. Vet. 18(2):78-83.
33. Smith M.C. & Roguinsky M. 1977. Mastitis and other disease of the goat́s udder. J. Am. Vet. Med. Assoc. 171:1241-1248.
34. Zeng S.S. 1996. Comparasion of goat milk standards with cow milk standads for analysis of somatic cell count, fat and protein in goat milk. Small Rum. Res. 21(3):221-225.
35. Zeng S.S., Escobar E.N., Hart S.P., Hinckley L., Baulthaus M., Robinson G.T. & Jahnke G. 1999. Comparative study of the effects of testing laboratory, counting method, storage and shipment on somatic cell counts in goat milk. Small Rumin. Res. 31:103-107.