Determination of temporal and spatial concentration gradients in hydrogel beads using multiphoton microscopy techniques

Applied Spectroscopy

Volumn 64, Issue 7, 2010, Pages 720-726

  Schwendt, Tilman ^a   Michalik, Claas ^a   Zavrel, Michael ^a   Dennig, Alexander ^a   Spiess, Antje C ^a   Poprawe, Reinhart ^a   Janzen, Christoph ^b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b FRAUNHOFER INSTITUTE FOR LASER TECHNOLOGY   (Germany)

Author keywords

Biochemical engineering; Enzyme immobilization; Hydrogel bead; Multiphoton microscopy

Indexed keywords

BIOCHEMICAL ENGINEERING; CHEMICAL COMPOUNDS; DATA ACQUISITION; DIFFUSION; ENZYME IMMOBILIZATION; ENZYMES; MASS TRANSFER; PHASE INTERFACES; REACTION KINETICS;

BIOTECHNOLOGICAL PROCESS; CONCENTRATION GRADIENTS; HYDROGEL BEADS; MECHANISTIC MODELING; MULTI-PHOTON MICROSCOPY; SPATIAL AND TEMPORAL RESOLUTIONS; TEMPORAL AND SPATIAL; TEMPORALLY RESOLVED;

HYDROGELS;

HYDROGEL; IMMOBILIZED ENZYME;

CHEMISTRY; DEVICES; DIFFUSION; EQUIPMENT DESIGN; HYDROGEL; KINETICS; MULTIPHOTON MICROSCOPY; PROCEDURES;

DIFFUSION; ENZYMES, IMMOBILIZED; EQUIPMENT DESIGN; HYDROGEL; KINETICS; MICROSCOPY, FLUORESCENCE, MULTIPHOTON;

EID: 77954866418     PISSN: 00037028     EISSN: None     Source Type: Journal    
DOI: 10.1366/000370210791666372     Document Type: Article

References (21)

1. W. Marquardt, Chem. Eng. Res. Design 83, 561 (2005).
2. K. Buchholz and U. Bornscheuer, Eng. Life Sci. 5, 309 (2005).
3. J. M. Woodley, Trends Biotechnol. 26, 321 (2008).
4. W. R. Berendsen, A. Lapin, and M. Reuss, Biotechnol. Prog. 22, 1305 (2006).
5. R. A. Sheldon, Adv. Synth. Catai. 349, 1289 (2007).
6. C. G. P. H. Schroen, C. B. Fretz, V. H. DeBruin, and W. R. Berendsen, et al., Biotechnol. Bioeng. 80, 331 (2002).
7. M. Zavřel, C. Michalik, T. Schwendt, T. W. Schmidt, M. B. AnsorgeSchumacher, C. Janzen, W. Marquardt, J. Buchs, and A. C. Spiess, "Model-based experimental analysis of enzyme kinetics in an aqueousorganic biphasic hydrogel system", Chem. Eng. Sei., paper in press (2010).
8. A. C. Spiess, M. Zavřel, M. B. Ansorge-Schumacher, C. Janzen, C. Michalik, T. W. Schmidt, T. Schwendt, J. Buchs, R. Poprawe, and W. Marquardt, Chem. Eng. Sci. 63, 3457 (2008).
9. B. L. Frazier, P. Larmour, and M. R. Riley, Biotechnol. Bioeng. 72, 364 (2001).
10. S. Kwak and M. Lafleur, Appl. Spectrosc. 57, 768 (2003).
11. M. Küppers, C. Heine, S. Han, S. Stapf, and B. Blümich, Appl. Magn. Reson. 22, 235 (2002).
12. M. Heinemann, T. Wagner, B. Doumèche, M. B. Ansorge-Schumacher, and J. Buchs, Biotechnol. Lett. 24, 845 (2002).
13. K. J. Halbhuber, Annal. Anatomy 185, 1 (2003).
14. S. W. Paddock, Biotechniques 27, 992 (1999).
15. S. R. Pygall, J. Whetstone, P. Timmins, and C. D. Melia, Adv. Drug Deliv. Rev. 59, 1434 (2007).
16. W. Lo, S. W. Teng, and H. Y. Tan, et al., Microsc. Res. Tech. 69, 973 (2006).
17. L. van den Berg, Y. Rosenberg, and M. A. J. S. van Boekel. Food Hydrocoll. 23, 1288 (2009).
18. X. H. Li, Y. F. Hua, A. Y. Qiu, C. Yang, and S. W. Cui, Food HydrocoU. 22, 845 (2008).
19. W. Denk, J. Strickler, and W. Webb, Science (Washington, D.C.) 248, 73 (1990).
20. F. Helmchen and W. Denk, Nature Methods 2, 932 (2005).
21. A. Diaspro, P. Bianchini, and G. Vicidomini, et al., Biomed. Eng. Online 5, 36 (2006).