Ectopic expression of hetP can partially bypass the need for hetR in heterocyst differentiation by Anabaena sp. strain PCC 7120

Molecular Microbiology

Volumn 77, Issue 3, 2010, Pages 562-574

  Higa, Kelly C ^a   Callahan, Sean M ^a  

^a UNIVERSITY OF HAWAII   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLENE; BACTERIAL PROTEIN; EXOPOLYSACCHARIDE; GLYCOLIPID; HETP PROTEIN; HETR PROTEIN; NITROGENASE; UNCLASSIFIED DRUG; HETP PROTEIN, ANABAENA SP.; HETR PROTEIN, BACTERIA; PROTEIN BINDING;

ANABAENA; ARTICLE; BACTERIAL CELL; CELL DIFFERENTIATION; ENZYME ACTIVITY; GENE DELETION; GENE EXPRESSION; HETEROCYST; HUMAN; INVERTED REPEAT; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN PROCESSING; SEQUENCE HOMOLOGY; AMINO ACID SEQUENCE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

ANABAENA SP.;

AMINO ACID SEQUENCE; ANABAENA; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; SEQUENCE ALIGNMENT;

EID: 77954846225     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2010.07257.x     Document Type: Article

References (35)

1. - mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing. J Bacteriol 176 : 2282 2292.
2. Black, T.A., Cai, Y. Wolk, C.P. (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9 : 77 84.
3. Borthakur, P.B., Orozco, C.C., Young-Robbins, S.S., Haselkorn, R. Callahan, S.M. (2005) Inactivation of patS and hetN causes lethal levels of heterocyst differentiation in the filamentous cyanobacterium Anabaena sp. PCC 7120. Mol Microbiol 57 : 111 123.
4. Buikema, W.J. Haselkorn, R. (1991) Characterization of a gene controlling heterocyst development in the cyanobacterium Anabaena 7120. Genes Dev 5 : 321 330.
5. Buikema, W.J. Haselkorn, R. (2001) Expression of the Anabaena hetR gene from a copper-regulated promoter leads to heterocyst differentiation under repressing conditions. Proc Natl Acad Sci USA 98 : 2729 2734.
6. Callahan, S.M. Buikema, W.J. (2001) The role of HetN in maintenance of the heterocyst pattern in Anabaena sp. PCC 7120. Mol Microbiol 40 : 941 950.
7. Ehira, S. Ohmori, M. (2006a) NrrA, a nitrogen-responsive response regulator facilitates heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Mol Microbiol 59 : 1692 1703.
8. Ehira, S. Ohmori, M. (2006b) NrrA directly regulates expression of hetR during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 188 : 8520 8525.
9. Ernst, A., Black, T., Cai, Y., Panoff, J.-M., Tiwari, D.N. Wolk, C.P. (1992) Synthesis of nitrogenase in mutants of the cyanobacterium Anabaena sp. strain PCC 7120 affected in heterocyst development or metabolism. J Bacteriol 174 : 6025 6032.
10. Fan, Q., Huang, G., Lechno-Yossef, S., Wolk, C.P., Kaneko, T. Tabata, S. (2005) Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120. Mol Microbiol 58 : 227 243. (Pubitemid 41415043)
11. Fellay, R., Frey, J. Krisch, H. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52 : 147 154.
12. Fernandez-Pinãs, F., Leganés, F. Wolk, C.P. (1994) A third genetic locus required for the formation of heterocysts in Anabaena sp. strain 7120. J Bacteriol 176 : 5277 5283.
13. Herrero, A., Muro-Pastor, A.M. Flores, E. (2001) Nitrogen control in cyanobacteria. J Bacteriol 183 : 411 425.
14. Higuchi, R., Krummel, B. Saiki, R.K. (1988) A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions. Nucleic Acids Res 16 : 7351 7367.
15. Holland, D. Wolk, C.P. (1990) Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts. J Bacteriol 172 : 3131 3137.
16. Huang, G., Fan, Q., Lechno-Yossef, S., Wojciuch, E., Wolk, C.P., Kaneko, T. Tabata, S. (2005) Clustered genes required for the synthesis of heterocyst envelope polysaccharide in Anabaena sp. strain PCC 7120. J Bacteriol 187 : 1114 1123. (Pubitemid 40189784)
17. Huang, X., Dong, Y. Zhao, J. (2004) HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc Natl Acad Sci USA 101 : 4848 4853.
18. Kaneko, T., Nakamura, Y., Wolk, C.P., Kuritz, T., Sasamoto, S., Watanabe, A., et al. (2001) Complete genome sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res 8 : 205 213 227 253.
19. Laurent, S., Chen, H., Bédu, S., Ziarelli, F., Peng, L. Zhang, C.-C. (2005) Nonmetabolizable analogue of 2-oxoglutarate elicits heterocyst differentiation under repressive conditions in Anabaena sp. PCC 7120. Proc Natl Acad Sci USA 102 : 9907 9912.
20. Miller, J.H. (1972) Experiments in Molecular Genetics.
21. Muro-Pastor, A.M., Valladares, A., Flores, E. Herrero, A. (2002) Mutual dependence of the expression of the cell differentiation regulatory protein HetR and the global nitrogen regulator NtcA during heterocyst development. Mol Microbiol 44 : 1377 1385.
22. Nayar, A.S., Yamaura, H., Rajagopalan, R., Risser, D.D. Callahan, S.M. (2007) FraG is necessary for filament integrity and heterocyst maturation in the cyanobacterium Anabaena sp. strain PCC 7120. Microbiology 153 : 601 607.
23. Orozco, C.C., Risser, D.D. Callahan, S.M. (2006) Epistasis analysis of four genes from Anabaena sp. strain PCC 7120 suggests a connection between PatA and PatS in heterocyst pattern formation. J Bacteriol 188 : 1808 1816.
24. Rajagopalan, R. Callahan, S.M. (2010) Temporal and spatial regulation of the four transcription start sites of hetR from Anabaena sp. strain PCC 7120. J Bacteriol 192 : 1088 1096.
25. Risser, D.D. Callahan, S.M. (2007) Mutagenesis of hetR reveals amino acids necessary for HetR function in the heterocystous cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 189 : 2460 2467.
26. Risser, D.D. Callahan, S.M. (2008) HetF and PatA control levels of HetR in Anabaena sp. strain PCC 7120. J Bacteriol 190 : 7645 7654.
27. Risser, D.D. Callahan, S.M. (2009) Genetic and cytological evidence that heterocyst patterning is regulated by inhibitor gradients that promote activator decay. Proc Natl Acad Sci USA 106 : 19884 19888.
28. Schaefer, M.R. Golden, S.S. (1989) Differential expression of members of a cyanobacterial psbA gene family in response to light. J Bacteriol 171 : 3973 3981.
29. Valladares, A., Flores, E. Herrero, A. (2008) Transcription activation by NtcA and 2-oxoglutarate of three genes involved in heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J Bacteriol 190 : 6126 6133.
30. Wei, T.-F., Ramasubramanian, R. Golden, J.W. (1994) Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J Bacteriol 176 : 4473 4482.
31. Wolk, C.P. (1996) Heterocyst formation. Annu Rev Genet 30 : 59 78.
32. Wolk, C.P. (2000) Heterocyst formation in Anabaena. In Prokaryotic Development. Brun, Y.V. Shimkets, L.J. (eds).
33. Xu, X. Wolk, C.P. (2001) Role for hetC in the transition to a nondividing state during heterocyst differentiation in Anabaena sp. J Bacteriol 183 : 393 396.
34. Yoon, H.-S. Golden, J.W. (1998) Heterocyst pattern formation controlled by a diffusible peptide. Science 282 : 935 938.
35. Yoon, H.-S. Golden, J.W. (2001) PatS and products of nitrogen fixation control heterocyst pattern. J Bacteriol 183 : 2605 2613.