Production of thymosin α 1 via non-enzymatic acetylation of the recombinant precursor

Biotechnology and Applied Biochemistry

Volumn 56, Issue 1, 2010, Pages 17-25

  Esipov, Roman S ^a   Stepanenko, Vasily N ^a   Beyrakhova, Ksenia A ^a   Muravjeva, Tatjana I ^a   Miroshnikov, Anatoly I ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

Escherichia coli expression system; Non enzymatic acetylation; Recombinant protein; Thymosin 1; Tobacco etch virus (TEV) protease

Indexed keywords

EXPRESSION SYSTEM; NON-ENZYMATIC; RECOMBINANT PROTEIN; TOBACCO ETCH VIRUS;

CHROMATOGRAPHY; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ION EXCHANGE; PEPTIDES; PURIFICATION; TOBACCO; ULTRAFILTRATION; VIRUSES;

ACETYLATION;

ACETIC ANHYDRIDE; DESACETYLTHYMOSIN ALPHA 1; HYBRID PROTEIN; RECOMBINANT THYMOSIN ALPHA1; THIOREDOXIN; THYMOSIN ALPHA1; UNCLASSIFIED DRUG; DRUG DERIVATIVE; PROTEINASE; TEV PROTEASE; THYMOSIN;

ACETYLATION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOTECHNOLOGICAL PRODUCTION; CANCER THERAPY; DRUG ACETYLATION; DRUG ISOLATION; DRUG MANUFACTURE; DRUG SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PLASMID; PRECURSOR; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ULTRAFILTRATION; BIOTECHNOLOGY; CHEMISTRY; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

ACETYLATION; BIOTECHNOLOGY; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; PLASMIDS; RECOMBINANT FUSION PROTEINS; THIOREDOXINS; THYMOSIN;

EID: 77954825954     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20100027     Document Type: Article

References (18)

1. Hooper, J. A., McDaniel, M. C., Thurman, G. B., Cohen, C. H., Schullof, R. S. and Goldstein, A. L. (1975) Ann. N.Y. Acad. Sci. 249, 125-144
2. Low, T. L., Thurman, G. B., McAdoo, M., McClure, J, Rossio, J. L., Naylor, P. H. and Goldstein, A. L. (1979) J. Biol. Chem. 254, 981-986
3. Romani, L., Bistoni, F., Montagnoli, C., Gaziano, R., Bozza, S., Bonifazi, P., Zelante, T., Moretti, S., Rasi, G., Garaci, E. and Puccetti, P. (2007) Ann. N. Y. Acad. Sci. 1112, 326-338
4. Sarandeses, C. S., Covelo, G., Díaz-Jullien, C. and Freire, M. (2003) J. Biol. Chem. 278, 13286-13293
5. Zhang, Y. Y., Chen, E. Q., Yang, J., Duan, Y. R. and Tang, H. (2009) Virol. J. 6, 63
6. Rustgi, V. K. (2007) Ann. N.Y. Acad. Sci. 1112, 357-367
7. Goldstein, A. L. and Goldstein, A. L. (2009) Expert Opin. Biol. Ther. 9, 593-608
8. Chen, Y., Zhao, L., Shen, G., Cui, L., Ren, W., Zhang, H., Qian, H. and Tang, K. (2008) Biotechnol. Appl. Biochem. 49, 51-56
9. Shmelev, V. A., Bunina, Z. F., Kudriavtseva, T. Iu., Zinchenko, E. V., Boldyreva, E. F. and Korobko, V. G. (1995) Mol. Gen. Mikrobiol. Virusol. 1, 9-14
10. Chen, P. F., Zhang, H. Y., Fu, G. F., Xu, G. X. and Hou, Y. Y. (2005) Acta Biochim. Biophys. Sin. 37, 147-151
11. Esipov, R. S., Gurevich, A. I., Stepanenko, V. N., Chupova, L. A., Chuvikovskii, D. V. and Miroshnikov, A. I. (2004) Bioorg. Khim. 30, 481-486
12. Wetzel, R., Heyneker, H. L., Goeddel, D. V., Jhurani, P., Shapiro, J., Crea, R., Low, T. L., McClure, J. E., Thurman, G. B. and Goldstein, A. L. (1980) Biochemistry 19, 6096-6104
13. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd, Cold Spring Harbor Laboratory Press, Plainview, NY
14. Laemmli, U. K. (1970) Nature 227, 680-685
15. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951) J. Biol. Chem. 193, 265-275
16. Zhou, L., Lai, Z. T., Lu, M. K., Gong, X. G. and Xie, Y. (2008) J. Biomed. Biotechnol. 2008, 736060
17. Wu, J., Chang, S., Gong, X., Liu, D. and Ma, Q. (2006) Biochim. Biophys. Acta 1760, 1241-1247
18. Fang, H., Zhang, X., Shen, L., Si, X., Ren, Y., Dai, H., Li, S., Zhou, C. and Chen, H. (2009) Appl. Microbiol. Biotechnol. 84, 99-104