Characterization of fecal microbiota in cats using universal 16S rRNA gene and group-specific primers for Lactobacillus and Bifidobacterium spp.

Veterinary Microbiology

Volumn 144, Issue 1-2, 2010, Pages 140-146

  Ritchie, Lauren E ^a   Burke, Kathrin F ^a   Garcia Mazcorro, Jose F ^a   Steiner, Jörg M ^a   Suchodolski, Jan S ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

16S rRNA gene; Bifidobacterium; Cat; Gastrointestinal tract; Lactobacillus; Microbiota

Indexed keywords

DNA; PRIMER RNA; RNA 16S;

ARTICLE; BACTERIUM DETECTION; BACTEROIDETES; BIFIDOBACTERIUM; CAT; CLOSTRIDIUM; DNA EXTRACTION; FECES CULTURE; FECES MICROFLORA; FIRMICUTES; GENE AMPLIFICATION; GENE SEQUENCE; LACTOBACILLUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PLASMID; PROTEOBACTERIA; SEQUENCE ANALYSIS; SPECIES DISTRIBUTION; SPECIES IDENTIFICATION;

ACTINOBACILLUS; ANIMALS; BASE SEQUENCE; BIFIDOBACTERIUM; CATS; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; FECES; FUSOBACTERIA; GENE AMPLIFICATION; LACTOBACILLUS; RNA, RIBOSOMAL, 16S;

ANIMALIA; BACTERIA (MICROORGANISMS); BACTEROIDETES; BIFIDOBACTERIUM; CLOSTRIDIUM; FELIDAE; FIRMICUTES; LACTOBACILLUS; PROTEOBACTERIA;

EID: 77954816352     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2009.12.045     Document Type: Article

References (25)

1. Atlas R., Bartha R. Microbial Ecology: Fundamentals and Applications 1998, Addison-Wesley Publishing Company, Reading, PA.
2. Daly K., Stewart C.S.F.H.J., Shirazi-Beechey S.P. Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes. FEMS Microbiol. Ecol. 2001, 38:141-151.
3. Desai A.R., Musil K.M., Carr A.P., Hill J.E. Characterization and quantification of feline fecal microbiota using cpn60 sequence-based methods and investigation of animal-to-animal variation in microbial population structure. Vet. Microbiol. 2008, 137:120-128.
4. Dethlefsen L., Huse S., Sogin M.L., Relman D.A. The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing. PLoS Biol. 2008, 6:e280.
5. Dowd S.E., Sun Y., Wolcott R.D., Domingo A., Carroll J.A. Bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) for microbiome studies: bacterial diversity in the ileum of newly weaned Salmonella-infected pigs. Foodborne Pathog. Dis. 2008, 5:459-472.
6. Falk P.G., Hooper L.V., Midtvedt T., Gordon J.I. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiol. Mol. Biol. Rev. 1998, 62:1157-1170.
7. Frank D.N., Amand A.L.S., Feldman R.A., Boedeker E.C., Harpaz N., Pace N.R. Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases. PNAS 2007, 104:13780-13785.
8. Good I.J. The population frequencies of species and the estimation of population parameters. Biometrica 1953, 40:237-264.
9. Hooper L.V., Wong M.H., Thelin A., Hansson L., Falk P.G., Gordon J.I. Molecular analysis of commensal host-microbial relationships in the intestine. Science 2001, 291:881-884.
10. Inness V.L., McCartney A.L., Khoo C., Gross K.L., Gibson G.R. Molecular characterisation of the gut microflora of healthy and inflammatory bowel disease cats using fluorescence in situ hybridisation with special reference to Desulfovibrio spp. J. Anim. Physiol. Anim. Nutr. 2007, 91:48-53.
11. Itoh K., Mitsuoka T., Maejima K., Hiraga C., Nakano K. Comparison of fecal flora of cats based on different housing conditions with special reference to Bifidobacterium. Lab. Anim. 1984, 18:280-284.
12. Johnston K.L., Lamport A., Batt R.M. An unexpected bacterial flora in the proximal small intestine of normal cats. Vet. Rec. 1993, 132:362-363.
13. Johnston K.L., Swift N.C., Forster-van Hijfte M., Rutgers H.C., Lamport A., Ballèvre O., Batt R.M. Comparison of the bacterial flora of the duodenum in healthy cats and cats with signs of gastrointestinal tract disease. J. Am. Vet. Med. Assoc. 2001, 218:48-51.
14. Lamendella R., Domingo J.W.S., Kelty C., Oerther D.B. Bifidobacteria in feces and environmental waters. Appl. Environ. Microbiol. 2008, 74:575-584.
15. Lubbs D.C., Vester B.M., Fastinger N.D., Swanson K.S. Dietary protein concentration affects intestinal microbiota of adult cats: a study using DGGE and PCR to evaluate differences in microbial populations in the feline gastrointestinal tract. J Anim Physiol Anim Nutri 2009, 93:113-121.
16. Osbaldiston G.W., Stowe E.C. Microflora of alimentary tract of cats. Am. J. Vet. Res. 1971, 32:1399-1405.
17. Packey C.D., Sartor R.B. Commensal bacteria, traditional and opportunistic pathogens, dysbiosis and bacterial killing in inflammatory bowel diseases. Curr. Opin. Infect. Dis. 2009, 22:292-301.
18. Papasouliotis K., Sparkes A.H., Werrett G., Egan K., Gruffydd-Jones E.A., Gruffydd-Jones T.J. Assessment of the bacterial flora of the proximal part of the small intestine in healthy cats, and the effect of sample collection method. Am. J. Vet. Res. 1998, 59:48-51.
19. Ritchie L., Steiner J., Suchodolski J. Assessment of microbial diversity along the feline intestinal tract using 16S rRNA gene analysis. FEMS Microbiol. Ecol. 2008, 66:590-598.
20. Sokol H., Pigneur B., Watterlot L., Lakhdari O., Bermudez-Humaran L.G., Gratadoux J.J., Blugeon S., Bridonneau C., Furet J.P., Corthier G., Grangette C., Vasquez N., Pochart P., Trugnan G., Thomas G., Blottiere H.M., Dore J., Marteau P., Seksik P., Langella P. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. PNAS 2008, 105:16731-16736.
21. Suau A., Bonnet R., Sutren M., Godon J.J., Gibson G.R., Collins M.D., Dore J. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl. Environ. Microbiol. 1999, 65:4799-4807.
22. Suchodolski J.S., Dowd S.E., Westermarck E., Steiner J.M., Wolcott R.D., Spillmann T., Harmoinen J.A. The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rDNA sequencing. BMC Microbiol. 2009, 10:210.
23. Suchodolski J.S., Ruaux C.G., Steiner J.M., Fetz K., Williams D.A. Assessment of the qualitative variation in bacterial microflora among compartments of the intestinal tract of dogs by use of a molecular fingerprinting technique. Am. J. Vet. Res. 2005, 66:1556-1562.
24. Suchodolski J., Camancho J., Steiner J. Analysis of bacterial diversity in the canine duodenum, jejunum, ileum, and colon by comparative 16S rRNA gene analysis. FEMS Microbiol. Ecol. 2008, 66:567-578.
25. Wang X., Heazlewood S.P., Krause D.O., Florin T.H.J. Molecular characterization of the microbial species that colonize human ileal and colonic mucosa by using 16S rDNA sequence analysis. J. Appl. Microbiol. 2003, 95:508-520.