Resolution of two native monomeric 90kDa nitrate reductase active proteins from Shewanella gelidimarina and the sequence of two napA genes

Biochemical and Biophysical Research Communications

Volumn 398, Issue 1, 2010, Pages 13-18

  Simpson, Philippa J L ^a   McKinzie, Audra A ^b   Codd, Rachel ^a,b  

^a UNIVERSITY OF SYDNEY   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

Author keywords

NAP isoforms; Periplasmic nitrate reductase; Shewanella

Indexed keywords

ISOPROTEIN; MONOMER; NITRATE REDUCTASE; NITRATE REDUCTASE ACTIVE PROTEIN; PROTEIN NAPA; PROTEIN NAPB; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; GENE SEQUENCE; MOLECULAR WEIGHT; NAPA GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROTEIN ANALYSIS; SEQUENCE ANALYSIS; SHEWANELLA; SHEWANELLA GELIDIMARINA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; GENES, BACTERIAL; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; NITRATE REDUCTASE; OPERON; SEQUENCE ALIGNMENT; SHEWANELLA;

BACTERIA (MICROORGANISMS); SHEWANELLA; SHEWANELLA GELIDIMARINA; SHEWANELLA PUTREFACIENS;

EID: 77954762742     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.06.002     Document Type: Article

References (25)

1. Moura J.J.G., Brondino C.D., Trincão J., Romão M.J. Mo and W bis-MGD enzymes: nitrate reductases and formate dehydrogenases. J. Biol. Inorg. Chem. 2004, 9:791-799.
2. McEwan A.G., Pridge J., McDevitt C.A. The DMSO reductase family of microbial molybdenum enzymes: molecular properties, and role in the dissimilatory reduction of toxic elements. Geomicrobiol. J. 2002, 19:3-21.
3. Dias J.M., Than M.E., Humm A., Huber R., Bourenkov G.P., Bartunik H.D., Bursakov S., Calvete J., Caldeira J., Carnerio C., Moura J.J.G., Moura I., Romão M.J. Crystal structure of the first dissimilatory nitrate reductase at 1.9Å solved by MAD methods. Structure 1999, 7:65-79.
4. Najmudin S., González P.J., Trincão J., Coelho C., Mukhopadhyay A., Cerqueira N.M.F.S.A., Romão C.C., Moura I., Moura J.J.G., Brondino C.D., Romão M.J. Periplasmic nitrate reductase revisited: a sulfur atom completes the sixth coordination of the catalytic molybdenum. J. Biol. Inorg. Chem. 2008, 13:737-753.
5. Jepson B.J.N., Mohan S., Clarke T.A., Gates A.J., Cole J.A., Butler C.S., Butt J.N., Hemmings A.J., Richardson D.J. Spectropotentiometric and structural analysis of the periplasmic nitrate reductase from Escherichia coli. J. Biol. Chem. 2007, 282:6425-6437.
6. Arnoux P., Sabaty M., Alric J., Frangioni B., Guigliarelli B., Adriano J.-M., Pignol D. Structural and redox plasticity in the heterodimeric periplasmic nitrate reductase. Nat. Struct. Biol. 2003, 10:928-934.
7. Marietou A., Richardson D.J., Cole J.A., Mohan S. Nitrate reduction by Desulfovibrio desulfuricans: a periplasmic nitrate reductase system that lacks NapB, but includes a unique tetraheme c-type cytochrome, NapM. FEMS Microbiol. Lett. 2005, 248:217-225.
8. Simpson P.J.L., Richardson D.J., Codd R. The periplasmic nitrate reductase in Shewanella: the resolution, distribution and functional implications of two NAP isoforms, NapEDABC and NapDAGHB. Microbiology 2010, 156:302-312.
9. Richardson D.J., Berks B.C., Russell D.A., Spiro S., Taylor C.J. Functional, biochemical and genetic diversity of prokaryotic nitrate reductases. Cell. Mol. Life Sci. 2001, 58:165-178.
10. Fredrickson J.K., Romine M.F., Beliaev A.S., Auchtung J.M., Driscoll M.E., Gardner T.S., Nealson K.H., Osterman A.L., Pinchuk G., Reed J.L., Rodionov D.A., Rodrigues J.L.M., Saffarini D.A., Serres M.H., Spormann A.M., Zhulin I.B., Tiedje J.M. Towards environmental systems biology of Shewanella. Nat. Rev. Microbiol. 2008, 6:592-603.
11. Hou H., Li L., Cho Y., de Figueiredo P., Han A. Microfabricated microbial fuel cell arrays reveal electrochemically active microbes. PLoS ONE 2009, 48:e6570.
12. Tiedje J.M. Shewanella-the environmentally versatile genome. Nat. Biotechnol. 2002, 20:1093-1095.
13. Bowman J.P., McCammon S.A., Nichols D.S., Skerratt J.S., Rea S.M., Nichols P.D., McMeekin T.A. Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel species with the ability to produce eicosapentaenoic acid (20:5ω3) and grow anaerobically with dissimilatory Fe(III) reduction. Int. J. Syst. Bacteriol. 1997, 47:1040-1047.
14. MacGregor C.H. Isolation and characterisation of nitrate reductase from Escherichia coli. Methods Enzymol. 1978, 53:347-355.
15. McEwan A.G., Jackson J.B., Ferguson S.J. Rationalization of properties of nitrate reductases in Rhodopsuedomonas capsulata. Arch. Microbiol. 1984, 137:344-349.
16. Kengen S.W.M., Rikken G.B., Hagen W.R., Van Ginkel C.G., Stams A.J.M. Purification and characterization of (per)chlorate reductase from the chlorate-respiring strain GR-1. J. Bacteriol. 1999, 181:6706-6711.
17. Hucklesby D.P., Hageman R.H. A staining method for nitrite reductase on polyacrylamide gels after electrophoresis. Anal. Biochem. 1973, 56:591-592.
18. Thomas P.E., Ryan D., Levin W. An improved staining procedure for the detection of the perioxidase activity of cytochrome P450 on sodium dodecyl sulfate polyacrylamide gels. Anal. Biochem. 1976, 75:168-176.
19. Butler C.S., Charnock J.M., Bennett B., Sears H.J., Reilly A.J., Ferguson S.J., Garner C.D., Lowe D.J., Thomson A.J., Berks B.C., Richardson D.J. Models for molybdenum coordination during the catalytic cycle of periplasmic nitrate reductase from Paracoccus denitrificans derived from EPR and EXAFS spectroscopy. Biochemistry 1999, 38:9000-9012.
20. 3 from Shewanella frigidimarina that is involved in Fe(III) respiration. Biochem. J. 2000, 349:153-158.
21. Ellington M.J.K., Fosdike W.L.J., Sawers R.G., Richardson D.J., Ferguson S.J. Regulation of the nap operon encoding the periplasmic nitrate reductase of Paracoccus pantotrophus: delineation of DNA sequences required for redox control. Arch. Microbiol. 2006, 184:298-304.
22. Cruz-Garcia C., Murray A.E., Klappenbach J.A., Stewart V., Tiedje J.M. Respiratory nitrate ammonification by Shewanella oneidensis MR-1. J. Bacteriol. 2007, 189:656-662.
23. Berks B.C., Richardson D.J., Robinson C., Reilly A., Aplin R.T., Ferguson S.J. Purification and characterization of the periplasmic nitrate reductase from Thiosphaera pantotropha. Eur. J. Biochem. 1994, 220:117-124.
24. Siddiqui R.A., Warnecke-Eberz U., Hengsberger A., Schneider B., Kostka S., Friedrich B. Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16. J. Bacteriol. 1993, 175:5867-5876.
25. Jepson B.J.N., Marietou A., Mohan S., Cole J.A., Butler C.S., Richardson D.J. Evolution of the soluble nitrate reductase: defining the monomeric periplasmic nitrate reductase subgroup. Biochem. Soc. Trans. 2006, 34:122-126.