Tear analysis of ascorbic acid, uric acid and malondialdehyde with capillary electrophoresis

Biomedical Chromatography

Volumn 24, Issue 8, 2010, Pages 852-857

  Georgakopoulos, Constantine D ^a   Lamari, Fotini N ^b   Karathanasopoulou, Ioanna N ^b   Gartaganis, Vassiliki S ^b   Pharmakakis, Nikolaos M ^a   Karamanos, Nikos K ^b  

^a UNIVERSITY OF PATRAS   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

Author keywords

Analysis; Ascorbic acid; Capillary electrophoresis; Malondialdehyde; Tears; Uric acid

Indexed keywords

ALDEHYDES; ANTIOXIDANTS; CAPILLARY ELECTROPHORESIS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ANALYSE; ANTIOXIDANT DEFENCE; ANTIOXIDANT MOLECULES; HIGH SENSITIVITY; LIPID PEROXIDATION; MALONDIALDEHYDE; OCULAR TISSUE; PATHOPHYSIOLOGICAL CHANGES; TEAR; URIC ACIDS;

ASCORBIC ACID;

ANTIOXIDANT; ASCORBIC ACID; EDETIC ACID; MALONALDEHYDE; URIC ACID;

ADULT; AGED; ANTIOXIDANT ACTIVITY; ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMICAL ANALYSIS; EXTRACTION; EYE DISEASE; HUMAN; LACRIMAL FLUID; LIPID PEROXIDATION; MEMBRANE FILTER; MOLECULE; NORMAL HUMAN; PATHOPHYSIOLOGY; SCHIRMER TEST; VALIDATION PROCESS; CHEMISTRY; EVALUATION STUDY; FEMALE; MALE; MIDDLE AGED; PROCEDURES;

ADULT; AGED; ANTIOXIDANTS; ASCORBIC ACID; ELECTROPHORESIS, CAPILLARY; FEMALE; HUMANS; MALE; MALONDIALDEHYDE; MIDDLE AGED; TEARS; URIC ACID;

EID: 77954750660     PISSN: 02693879     EISSN: 10990801     Source Type: Journal    
DOI: 10.1002/bmc.1376     Document Type: Article

References (17)

1. Abuja PM and Albertini R. Methods for monitoring oxidative stress, lipid peroxidation and oxidation resistance of lipoproteins, Clinica Chimica Acta 2001; 306(1-2): 1-17. (Pubitemid 32244320)
2. Anderson RE, Kretzer FL and Rapp LM. Free Radicals and ocular disease. Advances in Experimental Medicine and Biology 1994; 366: 73-86.
3. Behndig A, Svensson B, Marklund SL and Karlsson K. Superoxide dismutase isoenzymes in the human eye. Investigative Ophthalmology and Visual Science 1998; 39: 471-475. (Pubitemid 28110677)
4. Cheng ML, Liu TZ, Lu FJ and Chiu DT. Simultaneous detection of vitamin C and uric acid by capillary electrophoresis in plasma of diabetes and in aqueous humor in acute anterior uveitis. Clinical Biochemistry 1999; 32: 473-476.
5. Choy CKM, Benzie IFF and Cho P. Ascorbic acid concentration and total antioxidant activity of human tear fluid measured using the FRASC assay. Investigative Ophthalmology and Visual Science 2000; 41(11): 3293-3298.
6. Choy CKM, Cho P, Chung WY and Benzie IFF. Water-soluble antioxidants in human tears: effect of the collection method. Investigative Ophthalmology and Visual Science 2001; 42(13): 3130-3134. (Pubitemid 33740670)
7. Choy CK, Benzie IF and Cho P. Is ascorbate in human tears from corneal leakage or from lacrimal secretion? Clinical and Experimental Optometry 2004; 87(1): 24-27.
8. Crouch RK, Goletz P, Snyder A and Coles WH. Antioxidant enzymes in human tears. Journal of Ocular Pharmacology 1991; 7: 253-258.
9. Gogia R, Richer SP and Rose RC. Tear fluid content of electrochemically active components including water soluble antioxidants. Current Eye Research 1998; 17(3): 257-263. (Pubitemid 28138273)
10. Kopitz J, Holz FG, Kaemmerer E and Schutt F. Lipids and lipid peroxidation products in the pathogenesis of age-related macular degeneration. Biochimie 2004; 86(11): 825-831. (Pubitemid 39602762)
11. Korizis KN, Exarchou A, Michalopoulos E, Georgakopoulos CD, Kolonitsiou F, Mantagos S, Gartaganis SP and Karamanos NK. Determination of malondialdehyde by capillary electrophoresis, application to human plasma and relation of its levels with prematurity, Biomedical Chromatography 2001; 15: 287-291.
12. Matejckov J, Tuma P, Samcov E and Zemanov Z. Determination of uric acid in plasma and allantoic fluid of chicken embryos by capillary electrophoresis. Journal of Separation Science 2007; 30: 1947-1952.
13. Richer S. Antioxidants and the eye. International Ophthalmology Clinics 2000; 40(4): 1-16.
14. Ruiz-Jiménez J, Mata-Granados JM, Dolores M and de Castro L. On-line automatic SPE-CE coupling for the determination of biological markers in urine. Electrophoresis 2007; 28: 789-798. (Pubitemid 46404489)
15. Yao X., Wang Y and Chen G. Simultaneous determination of aminothiols, ascorbic acid and uric acid in biological samples by capillary electrophoresis with electrochemical detection. Biomedical Chromatography 2007; 21: 520-526. (Pubitemid 46736188)
16. Zinellu A, Carru C, Sotgia S and Deiana L. Optimization of ascorbic and uric acid separation in human plasma by free zone capillary electrophoresis ultraviolet detection. Analytical Biochemistry 2004; 330(2): 298-305. (Pubitemid 38760105)
17. Zinellu A, Sotgia S, Caddeo S, Deiana L and Carru C. Sodium glycylglycine as effective electrolyte run buffer for ascorbic and uric acid separation by CZE: a comparison with two other CE assays. Journal of Separation Science 2005; 28: 2193-2199. (Pubitemid 41700702)