Reduced susceptibility to ceftriaxone in Neisseria gonorrhoeae is associated with mutations G542S, P551S and P551L in the gonococcal penicillin-binding protein 2

Journal of Antimicrobial Chemotherapy

Volumn 65, Issue 8, 2010, Pages 1615-1618

  Whiley, David M ^a,b   Goire, Namraj ^a,b   Lambert, Stephen B ^a,b   Ray, Sanghamitra ^c   Limnios, E Athena ^c   Nissen, Michael D ^a,b,d   Sloots, Theo P ^a,b,d   Tapsall, John W ^c  

^a Queensland Children's Medical Research Institute   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c PRINCE OF WALES HOSPITAL   (Australia)

^d Pathology Queensland Central   (Australia)

Author keywords

Antibiotic resistance; Ceftriaxone; Gonorrhoea; PBP2; Pena

Indexed keywords

BACTERIAL PROTEIN; CEFTRIAXONE; GONOCOCCAL PENICILLIN BINDING PROTEIN 2; PENICILLIN G; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; PENICILLIN BINDING PROTEIN;

AMINO ACID SUBSTITUTION; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; GENE SEQUENCE; MINIMUM INHIBITORY CONCENTRATION; NEISSERIA GONORRHOEAE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; ANTIBIOTIC RESISTANCE; DNA SEQUENCE; DRUG EFFECTS; GENETICS; MICROBIAL SENSITIVITY TEST; MISSENSE MUTATION;

AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAM RESISTANCE; CEFTRIAXONE; MICROBIAL SENSITIVITY TESTS; MUTATION, MISSENSE; NEISSERIA GONORRHOEAE; PENICILLIN-BINDING PROTEINS; SEQUENCE ANALYSIS, DNA;

EID: 77954747876     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkq187     Document Type: Article

References (10)

1. Tapsall JW. Implications of current recommendations for third-generation cephalosporin use in the WHO Western Pacific region following the emergence of multi-resistant gonococci. Sex Transm Infect 2009; 85: 256-8.
2. Ito M, Deguchi T, Mizutani KS et al. Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in Central Japan. Antimicrob Agents Chemother 2005; 49: 137-43.
3. Lo JYC, Ho KM, Leung AOC et al. Ceftibuten resistance and treatment failure of Neisseria gonorrhoeae infection. Antimicrob Agents Chemother 2008; 52: 3564-7.
4. Lindberg R, Fredlund H, Nicholas R et al. Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponA. Antimicrob Agents Chemother 2007; 51: 2117-22.
5. Whiley DM, Limnios EA, Ray S et al. Diversity of penA alterations and subtypes in Neisseria gonorrhoeae strains from Sydney, Australia, that are less susceptible to ceftriaxone. Antimicrob Agents Chemother 2007; 51: 3111-6.
6. Powell AJ, Tomberg J, Deacon AM et al. Crystal structures of penicillin-binding protein 2 from penicillin-susceptible and -resistant strains of Neisseria gonorrhoeae reveal an unexpectedly subtle mechanism for antibiotic resistance. J Biol Chem 2009; 284: 1202-12.
7. Zhao S, Duncan M, Tomberg J et al. Genetics of chromosomally mediated intermediate resistance to ceftriaxone and cefixime in Neisseria gonorrhoeae. Antimicrob Agents Chemother 2009; 53: 3744-51.
8. Lee SG, Lee H, Jeong SH et al. Various penA mutations together with mtrR, porB and ponA mutations in Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone. J Antimicrob Chemother 2010; 65: 669-75.
9. Tapsall JW, Ray S, Limnios A. Characteristics and population dynamics of mosaic penA allele-containing Neisseria gonorrhoeae isolated in Sydney, Australia, 2007-2008. Antimicrob Agents Chemother 2010; 54: 554-6.
10. Kugelman G, Tapsall JW, Goire N et al. Simple, rapid, and inexpensive detection of Neisseria gonorrhoeae resistance mechanisms using heat-denatured isolates and SYBR green-based real-time PCR. Antimicrob Agents Chemother 2009; 53: 4211-6.