Preparation of carboxylated magnetic particles for the eYcient immobilization of C-terminally lysine-tagged Bacillus stearothermophilus aminopeptidase II

Journal of Industrial Microbiology and Biotechnology

Volumn 37, Issue 7, 2010, Pages 717-725

  Huang, Cheng Liang ^a   Cheng, Wei Chun ^a   Yang, Jia Ci ^a   Chi, Meng Chun ^a   Chen, Jiau Hua ^b   Lin, Hong Ping ^c   Lin, Long Liu ^a  

^a NATIONAL CHIAYI UNIVERSITY   (Taiwan)

^b CHIA NAN UNIVERSITY OF PHARMACY AND SCIENCE   (Taiwan)

^c NATIONAL CHENG KUNG UNIVERSITY   (Taiwan)

Author keywords

Aminopeptidase II; Bacillus stearothermophilus; Immobilization; Lysine tag; Magnetic particles

Indexed keywords

AMINOPEPTIDASE; AMINOPEPTIDASE 2; LYSINE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; IMMOBILIZED ENZYME; MAGNETITE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CARBOXYLATION; CATALYSIS; ENZYME KINETICS; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; TRANSMISSION ELECTRON MICROSCOPY; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; PROTEIN TERTIARY STRUCTURE; TEMPERATURE;

AMINOPEPTIDASES; BACTERIAL PROTEINS; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FERROSOFERRIC OXIDE; GEOBACILLUS STEAROTHERMOPHILUS; PROTEIN STRUCTURE, TERTIARY; TEMPERATURE;

GEOBACILLUS STEAROTHERMOPHILUS;

EID: 77954689609     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-010-0715-8     Document Type: Article

References (31)

1. Arnau J, Lauritzen C, Petersen GE, Pedersen J (2006) Current strategies for the use of aYnity tags and tag removal for the puri- Wcation of recombinant proteins. Protein Expr Purif 48:1-13
2. Salemuddin M (1999) BioaYnity based immobilization of enzymes. In: Fiechter A (ed) Advances in biochemical engineering/ biotechnology, vol 64. Springer, Berlin, pp 203-226
3. Bahar T, Celebi SS (1999) Immobilization of glucoamylase on magnetic poly(styrene) particles. J Appl Polym Sci 72:69-73
4. Koneracká M, Kopbanský P, Timko M, Ramchand CN, de Sequeira A, Trevan M (2002) Direct binding procedure of proteins and enzymes to Wne magnetic particles. J Mol Catal B: Enzym 18:13-18
5. Lei H, Wang W, Chen LL, Li XC, Yi B, Deng L (2004) The preparation and catalytically active characterization of papain immobilized on magnetic composite microspheres. Enzyme Microb Technol 35:15-21
6. Chien LJ, Lee CK (2008) BiosiliciWcation of dual-fusion enzyme immobilized on magnetic nanoparticle. Biotechnol Bioeng 100:223-230
7. Lo HF, Hu HY, Hung CP, Chen SC, Lin LL (2009) Cobalt-chelated magnetic particles for one-step puriWcation and immobilization of His6-tagged Escherichia coliγ-glutamyltranspeptidase. Biocatal Biotransform 27:318-327
8. Hanefeld U, Gardossi L, Magner E (2009) Understanding enzyme immobilization. Chem Soc Rev 38:453-468
9. Durán N, Rosa MA, D'Annibale A, Gianfreda L (2002) Applications of laccases and tyrosinases (phenoloxidase) immobilized on diVerent supports: a review. Enzyme Microb Technol 31:907-931
10. Girelli AM, Mattei J (2005) Application of immobilized enzyme reactor in on-line high performance liquid chromatography: a review. J Chromatogr B 819:3-16
11. Wang G, Xu JJ, Ye LH, Zhu JJ, Chen HY (2002) Highly sensitive sensors based on the immobilization of tyrosinase in chitosan. Bioelectrochemistry 57:33-38
12. PetroviT J, Clark RA (2005) Impact of surface immobilization and solution ionic strength on the formal potential of immobilized cytochrome. Langmuir 21:6308-6316
13. Goldberg AL, Cascio P, Saric T, Rock KL (2002) The importance of the proteasome and subsequent proteolytic steps in the generation of antigenic peptides. Mol Immunol 39:147-164
14. Terenius L, Sandin J, Sakurada T (2002) Nociceptin/orphanin FQ metabolism and bioactive metabolites. Peptides 21:919-922
15. Pautot V, Holzer FM, Reisch B, Walling LL (1993) Leucine aminopeptidase: an inducible component of the defense response in Lycopersion esculentum (tomato). Proc Natl Acad Sci USA 90:9906-9910
16. Fitzgerald RJ, O'Cuinn G (2006) Enzymatic debittering of food protein hydrolysates. Biotechnol Adv 24:234-237
17. Wyvratt MJ (1988) Evolution of angiotensin-converting enzyme inhibitors. Clin Physiol Biochem 6:217-229
18. Kuo LY, Hwang GY, Lai YJ, Yang SL, Lin LL (2003) Overexoression, puriWcation, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilus. Curr Microbiol 47:40-45
19. Kuo LY, Hwang GY, Yang SL, Hua YW, Chen W, Lin LL (2004) Inactivation of Bacillus stearothermophilus leucine aminopeptidase II by hydrogen peroxide and site-directed mutagenesis of methionine resiudes on the enzyme. Protein J 23:295-302
20. Hwang GY, Kuo LY, Tsai MR, Yang SL, Lin LL (2005) Histidines 345 and 378 of Bacillus stearothermophilus leucine aminopeptidase II are essential for the catalytic activity of the enzyme. Antonie van Leeuwenhoek 87:355-359
21. Yang SL, Chen RS, Chen W, Lin LL (2006) Identification of glutamate residues important for catalytic activity of Bacillus stearothermophilus leucine aminopeptidase II. Antonie van Leeuwenhoek 90:195-199
22. Wu CL, Chen YP, Yang JC, Lo HF, Lin LL (2008) Characterization of lysine-tagged Bacillus stearothermophilus leucine aminopeptidase II immobilized onto carboxylated gold nanoparticles. J Mol Catal B: Enzym 54:83-89
23. Stoll E, Weder HG, Zuber H (1976) Aminopeptidase II from Bacillus stearothermophilus. Biochem Biophys Acta 438:212-220
24. Khalafalla SE, Reimers GW (1980) Preparation of dilution-stable aqueous magnetic Xuids. IEEE Trans Magn 16:178-183
25. Tsang SC, Yu CH, Gao X, Tam K (2006) Silica-encapsulated nanomagnetic particle as a new recoverable biocatalyst carrier. J Phys Chem B 110:16914-16922
26. Li J, Wang J, Gavalas VG, Atwood DA, Bachas LG (2003) Alumina-pepsin hybrid nanoparticles with orientation-speciWc enzyme coupling. Nano Lett 3:55-58
27. Takahashi H, Li B, Sasaki T, Miyazaki C, Kajino T, Inagaki S (2000) Catalytic activity in organic solvents and stability of immobilized enzymes depend on the pore size and surface characteristics of mesoporous silica. Chem Mater 12:3301-3305
28. Akgol S, Yalcinkaya Y, Bayramoglu A, Denizli A, Arica MY (2002) Reversible immobilization of urease onto Procion Brown MX-5BR-Ni(II) attached polyamide hollow-Wbre membranes. Process Biochem 38:675-683
29. Li GY, Huang KL, Jiang YR, Yang DL, Ding P (2008) Preparation and characterization of Saccharomyces cerevisiae alcohol dehydrogenase immobilized on magnetic nanoparticles. Int J Biol Macromol 42:405-412
30. Liao MH, Chen DH (2001) Immobilization of yeast alcohol dehydrogenase on magnetic nanoparticles for improving its stability. Biotechnol Lett 23:3234-3241
31. Chen DH, Liao MH (2002) Preparation and characterization of YADH-bound magnetic nanoparticles. J Mol Catal B: Enzym 16:283-291