Detection of Thielaviopsis basicola in soil with real-time quantitative PCR assays

Microbiological Research

Volumn 165, Issue 5, 2010, Pages 411-417

  Huang, Junli ^a   Kang, Zhenhui ^a  

^a CHONGQING UNIVERSITY   (China)

Author keywords

Detection; Real time quantitative PCR; Soil; Thielaviopsis basicola

Indexed keywords

AGRICULTURAL CROPS; BLACK ROOT ROT; DETECTION; DETECTION LIMITS; EARLY DIAGNOSIS; GENOMIC DNA; IN-FIELD; INFESTED SOILS; INTERNAL TRANSCRIBED SPACERS; NUCLEOTIDE SEQUENCES; QUANTITATIVE PCR; REAL-TIME DETECTION; REAL-TIME QUANTITATIVE PCR; SENSITIVE DETECTION; SOILBORNE FUNGUS; TAQMAN PROBES; TOBACCO PLANTS;

CROPS; DIAGNOSIS; DISEASE CONTROL; DNA; DNA SEQUENCES; GENES; POLYMERASE CHAIN REACTION; TOBACCO;

SOILS;

DETECTION METHOD; DISEASE CONTROL; FUNGUS; GENETIC ANALYSIS; HOST RANGE; POLYMERASE CHAIN REACTION; POPULATION DISTRIBUTION; PROBE; REAL TIME; RELIABILITY ANALYSIS; ROOT ROT; SOIL MICROORGANISM; SOIL SURVEY; TOBACCO;

CHINA;

FUNGI; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; THIELAVIOPSIS; THIELAVIOPSIS BASICOLA;

FUNGAL DNA; PRIMER DNA;

ARTICLE; ASCOMYCETES; CLASSIFICATION; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; POLYMERASE CHAIN REACTION;

ASCOMYCOTA; DNA PRIMERS; DNA, FUNGAL; POLYMERASE CHAIN REACTION; SOIL MICROBIOLOGY;

EID: 77954668069     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2009.09.001     Document Type: Article

References (24)

1. Bosshard E, Heller W. Black root rot of Ribes spp. in Switzerland. In: Brennan RM, Gordon SL, Williamson B, editors., Acta Horticulturae Dundee, Scotland; 2002, p. 335-6.
2. Chittaranjan S., Punja Z.K. A semiselective medium and procedures for isolation and enumeration of Chalara elegants from organic soil. Plant Dis 1993, 77:930-932.
3. de Weerdt M., Zijlstra C., van Brouwershaven I.R., van Leeuwen G.C.M., de Gruyter J., Kox L.F.F. Molecular detection of Fusarium foetens in Begonia. J Phytopathol 2006, 154:694-700.
4. Edgcomb V.P., McDonald J.H., Devereux R., Devereux R., Smith D.W. Estimation bacterial cell numbers in humic-rich salt marsh sediments using probes to 16S rDNA. Appl Envir Microbiol 1999, 65:1516-1523.
5. Francois C., Castagnone C., Boonham N., Tomlinson J., Lawson R., Hockland S., et al. Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus. Mol Plant Pathol 2007, 8:803-809.
6. Geldenhuis M.M., Roux J.A., Wingfield M.J., Wingfield B.D. Development of polymorphic markers for the root pathogen Thielaviopsis basicola using ISSR-PCR. Mol Ecol Notes 2004, 4:547-550.
7. Gerhardt D.J., Phibbs A., Charkowski A.O. Comparison of ELISA, PCR, and a new TaqMan real-time PCR test for Clavibacter michiganense subsp sepedonicus. Phytopathology 2008, 98(6):S59.
8. Hughes K.J.D., Tomlinson J.A., Griffin R.L., Boonham N., Inman A.J., Lane C.R. Development of a one-step realtime polymerase chain reaction assay for diagnosis of Phytophthora ramorum. Phytopathology 2006, 96:975-981.
9. Ippolito A., Schena L., Nigro F., Ligorio V.S., Yaseen T. Real-time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil. Eur J Plant Pathol 2004, 110:833.
10. Koike S.T., Henderson D.M. Black root rot, caused by Thielaviopsis basicola, on tomato transplants in California. Plant Dis 1998, 82:447. (Abstr.).
11. Kuske C.R., Barns S.M., Busch J.D. Diverse uncultivated bacterial groups from soils of the arid southwestern United States that are present in many geographic regions. Appl Environ Microbiol 1997, 63:3614-3621.
12. Labuschagne N., Kotze J.M. Incidence of Chalara elegans in groundnut seed samples and seed transmission of blackhull. Plant Pathol 1991, 40:639-642.
13. Lee S.B., Taylor J.W. Isolation of DNA from fungal mycelia and single spores. PCR protocols: a guide to methods and applications 1990, 282-287. Academic Press, San diego, California, USA, London, England, UK. M.A. Innis (Ed.).
14. Maria M.G., Jolanda R., Andre J.C., Brenda D.W., Michael J.W. Clonality in South African isolates and evidence for a European origin of the root pathogen Thielaviopsis basicola. Mycological Res 2006, 161:306-311.
15. Malvick D.K., Impullitti A.E.. Detection and quantification of Phialophora gregata in soybean and soil samples with a quantitative, real-time PCR assay. Plant Dis 2007, 91:736-742.
16. Nehl D.B., Allen S.J., Mondal A.H., Lonergan P.A. Black root rot: a pandemic in Australian cotton. Australas Plant Pathol 2004, 33:87-95.
17. Punja Z.K., Chittaranjan S., Gaye M. Development of black root rot caused by Chalara elegans of fresh market carrots. Can J Plant Pathol 1992, 14:299-309.
18. Rao Y.Y., Sang W.J., Jiang C.Y., Wu W.C. Occurring character and control technology of Thielariopsis basicola of the mulching flue-cured tobacco in Hezhang Guizhou. J Mountain Agricul Biol 2006, 25:370-372.
19. Specht L.P., Griffin G.J. A selective medium for enumerating low populations of Thielaviopsis basicola in tobacco field soils. Can J Plant Pathol 1985, 7:438-441.
20. Stanghellini M.E., Rasmussen S.L., Kim D.H. Aerial transmission of Thielaviopsis basicola, a pathogen of corn-salad, by adult shore flies. Phytopathology 1999, 89:476-479.
21. Sun D., Tang L.L., Wang Q.Q., Yang D.M., Jiang C.J., Wu B. Evaluation and optimization of DNA extraction and purification from hypersaline environmental samples and construction of metagenomic library. Guangxi Agric Bio Sci 2006, 25:24-29.
22. Thalmann R., Kaufmann K., Struck C. Black root rot on lupins-an early species-specific detection of the pathogen Thielaviopsis basicola. Gesunde Pflanzen 2008, 60:67-75.
23. Zhao Y.Q., Zhang C.L., Zhang W., Li L.N., Zhang G.M. Molecular detection of Thielaviopsis basicola by PCR assay. Acta Phytopathology Sinica 2009, 39:23-29.
24. Zhu C.X., Wang Y.T., Wang Z.F. China tobacco diseases (in Chinese) 2001, China Agricultural Press, Beijing, China.