Knowledge-based design of reagentless fluorescent biosensors from a designed ankyrin repeat protein

Protein Engineering, Design and Selection

Volumn 23, Issue 4, 2010, Pages 229-241

  Brient Litzler, Elodie ^a   Plückthun, Andreas ^b   Bedouelle, Hugues ^a  

^a INSTITUT PASTEUR   (France)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

ankyrin repeat protein (DARPin); antibody; biosensor; fluorescence; protein design

Indexed keywords

ANALYTICAL SCIENCE; ANKYRIN; ANKYRIN REPEAT PROTEIN (DARPIN); ANTIGEN BINDING; COMBINATORIAL LIBRARY; CYSTEINE RESIDUES; DIRECT CONTACT; FLUORESCENCE INTENSITIES; HIGH YIELD; LOWER LIMIT OF DETECTIONS; MALTOSE BINDING PROTEINS; PROTEIN DESIGN; REAGENT-LESS; RECOMBINANT EXPRESSION; RELATIVE SENSITIVITY; SOLVENT ACCESSIBLE SURFACE AREAS; SPECTROFLUOROMETERS;

ANTIBODIES; ANTIGENS; BIOSENSORS; BODY FLUIDS; CRYSTAL STRUCTURE; DESIGN; ESCHERICHIA COLI; KNOWLEDGE BASED SYSTEMS; POTASSIUM; POTASSIUM IODIDE;

FLUORESCENCE;

ANKYRIN; ANTIGEN; BUFFER; CYSTEINE; DESIGNED ANKYRIN REPEAT PROTEIN; MALTOSE BINDING PROTEIN; OFF7 PROTEIN; POTASSIUM IODIDE; SOLVENT; UNCLASSIFIED DRUG; FLUORESCENT DYE; MALTOSE-BINDING PROTEIN; PERIPLASMIC BINDING PROTEIN;

ANTIGEN BINDING; ARTICLE; BIOSENSOR; COMBINATORIAL LIBRARY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE; FLUOROMETER; NONHUMAN; PRIORITY JOURNAL; SPECTROFLUOROMETRY; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; GENETIC PROCEDURES; GENETICS; KNOWLEDGE BASE; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI;

ANKYRIN REPEAT; BINDING SITES; BIOSENSING TECHNIQUES; CRYSTALLOGRAPHY, X-RAY; CYSTEINE; ESCHERICHIA COLI; FLUORESCENT DYES; KNOWLEDGE BASES; MODELS, MOLECULAR; PERIPLASMIC BINDING PROTEINS; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

EID: 77954639937     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp074     Document Type: Article

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