Efficient method for production of high yields of Fab fragments in Drosophila S2 cells

Protein Engineering, Design and Selection

Volumn 23, Issue 4, 2010, Pages 169-174

  Backovic, Marija ^a   Johansson, Daniel X ^b   Klupp, Barbara G ^c   Mettenleiter, Thomas C ^c   Persson, Mats A A ^b   Rey, Felix A ^a  

^a INSTITUT PASTEUR   (France)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

Drosophila S2 cells; expression; Fab; insect cells; monoclonal antibodies

Indexed keywords

BIOCHEMICAL CHARACTERIZATION; CELL LINES; COGNATE ANTIGENS; COST-EFFECTIVE METHODS; DROSOPHILA S2 CELLS; EFFICIENT METHOD; ENZYMATIC DIGESTIONS; EXPRESSION; FAB; FAB FRAGMENTS; GLYCOSYLATED; HIGH YIELD; HYBRIDOMA CELLS; INSECT CELLS; STRUCTURAL BIOLOGY; THERAPEUTIC AGENTS;

ALLERGIES; CELLS; MERGERS AND ACQUISITIONS; MONOCLONAL ANTIBODIES; SIZE EXCLUSION CHROMATOGRAPHY; SURFACE PLASMON RESONANCE;

CELL CULTURE;

IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; HYBRID PROTEIN;

ANIMAL CELL; ARTICLE; CELL STRAIN S2; CONTROLLED STUDY; DROSOPHILA; GEL PERMEATION CHROMATOGRAPHY; HYBRID CELL; HYBRIDOMA CELL CULTURE; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; ANIMAL; BIOSYNTHESIS; CELL LINE; CHEMISTRY; CYTOLOGY; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METABOLISM; MOUSE;

HEXAPODA;

ANIMALS; CELL LINE; DROSOPHILA; GENETIC VECTORS; IMMUNOGLOBULIN FAB FRAGMENTS; MICE; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 77954635186     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp088     Document Type: Article

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