Generation of an antibody against the protein phosphatase 1 inhibitor KEPI and characterization of the epitope

Anticancer Research

Volumn 30, Issue 5, 2010, Pages 1573-1578

  Daskalow, Katjana ^a,b,e   Boisguerin, Prisca ^c   Jandrig, Burkhard ^b   Van Landeghem, Frank K H ^d   Volkmer, Rudolf ^c   Micheel, Burkhard ^a   Schenk, Jörg A ^a  

^a UNIVERSITY OF POTSDAM   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^d UNIVERSITY HOSPITAL BONN   (Germany)

^e AJ Innuscreen GmbH   (Germany)

Author keywords

Hybridoma; KEPI; Monoclonal; Peptide array; Substitutional analysis

Indexed keywords

ANTIBODY; EPITOPE; G18AF8 ANTIBODY; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY KEPI; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN KEPI; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL EXPERIMENT; ANIMAL TISSUE; BRAIN; CHICKEN; CONFERENCE PAPER; ESCHERICHIA COLI; FEMALE; HAMSTER; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; RAT;

AMINO ACIDS; ANIMALS; ANTIBODIES, MONOCLONAL; BRAIN; EPITOPE MAPPING; EPITOPES; FEMALE; HUMANS; HYBRIDOMAS; MICE; MICE, INBRED BALB C; PEPTIDES; PROTEIN BINDING; PROTEIN PHOSPHATASE 1; TISSUE DISTRIBUTION;

EID: 77954585806     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (16)

1. Liu QR, Zhang PW, Zhen Q, Walther D, Wang XB and Uh1 GR: KEPI, a PKC-dependent protein phosphatase 1 inhibitor regulated by morphine. J Biol Chem 277: 13312-13320, 2002. (Pubitemid 34952703)
2. Caselli R, Mencarelli MA, Papa FT, Uliana V, Schiavone S, Strambi M, Pescucci C, Ariani F, Rossi V, Longo I, Meloni I, Renieri A and Mari F: A 2.6 Mb deletion of 6q24.3-25.1 in a patient with growth failure, cardiac septal defect, thin upper lip and asymmetric dysmorphic ears. Eur J Med Genet 50: 315-321, 2007. (Pubitemid 47058413)
3. Theile M, Seitz S, Arnold W, Jandrig B, Frege R, Schlag PM, Haensch W, Guski H, Winzer KJ, Barrett JC and Scherneck S: A defined chromosome 6q fragment (at D6S310) harbors a putative tumor suppressor gene for breast cancer. Oncogene 13: 677-685, 1996. (Pubitemid 26312894)
4. Bonazzi VF, Irwin D and Hayward NK: Identification pf candidate tumor suppressor genes inactivated by promoter methylation in melanoma. Genes Chromosomes Cancer 48: 10-21, 2009.
5. Wenzel K, Daskalow K, Herse F, Seitz S, Zacharias U, Schenk JA, Schulz H, Hubner N, Micheel B, Schlag PM, Osterziel KJ, Ozcelik C, Scherneck S and Jandrig B: Expression of the protein phosphatase 1 inhibitor KEPI is down-regulated in breast cancer cell lines and tissues and involved in the regulation of the tumor suppressor EGR1 via the MEK-ERK pathway. Biol Chem 388: 489-495, 2007. (Pubitemid 46800266)
6. Daskalow K, Boisguerin P, Jandrig B, Volkmer R, Micheel B and Schenk JA: Epitope mapping of antibodies against S-tagged fusion proteins and molecular weight markers. Biosci Biotechnol Biochem 72: 346-351, 2008. (Pubitemid 351353649)
7. Schenk JA, Matyssek F and Micheel B: Interleukin 4 increases the antibody response against rubisco in mice. In Vivo 18: 649-652, 2004.
8. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
9. Luebbe L, Schenk JA, Naundorf H, Karsten U and Wunderlich V: Reverse transformation of human mammary carcinoma cells. Anticancer Res 19: 5119-5125, 1999. (Pubitemid 30103333)
10. Sheriff A, Vogt B, Baumgart M, Montag C, Hollenbach B, Schenk JA, Ulrich J, Elías F and Micheel B: Intracellular capture of B7 in antigen-presenting cells reduces costimulatory activity. Biochem Biophys Res Commun 301: 873-878, 2003. (Pubitemid 36279194)
11. Frank R: The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports - principles and applications. J Immunol Methods 267: 13-26, 2002.
12. Boisguerin P, Leben R, Ay B, Radziwill G, Moelling K, Dong L and Volkmer-Engert R: An improved method for the synthesis of cellulose membrane-bound peptides with free C termini is useful for PDZ domain-binding studies. Chem Biol 11: 449-459, 2004.
13. Gong JP, Liu QR, Zhang PW, Wang Y and Uhl GR: Mouse brain localization of the protein kinase C-enhanced phosphatase 1 inhibitor KEPI (kinase C-enhanced PP1 inhibitor). Neuroscience 132: 713-727, 2005.
14. Erdodi F, Kiss E, Walsh MP, Stefansson B, Deng JT, Eto M, Brautigan DL and Hartshorne DJ: Phosporylation of protein phosphatase type-1 inhibitory proteins by integrin-linked kinase and cyclic nucleotide-dependent protein kinases. Biochem Biophys Res Commun 306: 382-387, 2003.
15. Drgonova J, Zimonjic DB, Hall FS and Uhl GR: Effect of KEPI (Ppp1r14c) deletion on morphine analgesia and tolerance in mice of different genetic backgrounds: when a knockout is near a relevant quantitative trait locus. Neuroscience 165: 882-895, 2010.
16. Eto M: Regulation of cellular protein phosphatase-1 (PP1) by phosphorylation of the CPI-17 family, C-kinase-activated PP1 inhibitors. J Biol Chem 284: 35273-35277, 2009.