Improved HPLC method for the evaluation of the major steviol glycosides in leaves of Stevia rebaudiana

European Food Research and Technology

Volumn 231, Issue 4, 2010, Pages 581-588

  Woelwer Rieck, Ursula ^a   Lankes, Christa ^a   Wawrzun, Andreas ^a   Wüst, Matthias ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

HILIC analytical column; Rebaudioside A; Reversed phase HPLC; Stevia rebaudiana; Steviol glycosides; Stevioside

Indexed keywords

ANALYTICAL COLUMNS; REBAUDIOSIDE-A; REVERSED PHASE HPLC; STEVIA REBAUDIANA; STEVIOSIDES;

CHROMATOGRAPHIC ANALYSIS; HARVESTING; OLEFINS; PROJECTILES; SOLVENT EXTRACTION; SUGARS;

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

STEVIA REBAUDIANA;

EID: 77954533178     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-010-1309-4     Document Type: Article

References (38)

1. Ramesh K, Singh V, Megeji NW (2006) Cultivation of Stevia (Stevia rebaudiana Bertoni): a comprehensive review. Adv Agron 89: 137-177.
2. Kinghorn AD (2002) Overview. In: Kinghorn AD (ed) Stevia, the Genus of Stevia medicinal and aromatic plants-industrial profiles. Taylor and Francis, London.
3. Makapuguay HC, Nanyakkara NPD, Kinghorn AD (1984) Improved high-performance liquid chromatographic separation of the Stevia rebaudiana sweet terpene glycosides using a linear gradient elution. J Chromatog 283: 390-395.
4. Geuns JMC (2003) Molecules of interest: Stevioside. Phytochemistry 64: 913-921.
5. Starratt AN, Kirby CW, Pocs R, Brandle JE (2002) Rebaudioside F, a diterpene glycoside from Stevia rebaudiana. Phytochemistry 59: 367-370.
6. Kim SH, Dubois GF (1991) Natural high potency sweeteners. In: Marie S, Piggott JR (eds) Handbook of sweeteners. Blacki Avi. Glasgow.
7. Prakash I, DuBois GE, Clos JF, Wilkens KL, Fosdick LE (2008) Development of rebiana, a natural, non-caloric sweetener. Food ChemToxicol 46: S75-S82.
8. Cargill GRAS Notification for Rebaudioside A (2008) GRAS Notification 253 http://www. fdagov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGRAS/GRASListings/ucm154989htm. Accessed 5 Jan 2010.
9. Merisant GRAS Notification for Rebaudioside A (2008) GRAS Notification 252 http://www. fdagov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGR AS/GRASListings/ucm154988htm. Accessed 5 Jan 2010.
10. Wisdom GRAS Notification for steviol glycosides with rebaudioside A and stevioside as principal components (2009) GRAS Notification 287 http://www. fdagov/Food/FoodIngredientsPackaging/GenerallyRecognizedasSafeGRAS/GRASListings/ucm181937htm. Accessed 5 Jan 2010.
11. JECFA (2007) Steviol glycosides. In: Combined compendium of food additive specifications, 68th meeting of the joint FAO/WHO expert committee on food additives, FAO/JECFA monograph 4, Rome. http://www. faoorg/ag/agn/jecfa-additives/specs/monograph4/additive-442-m4pdf. Accessed 13 Jan 2010.
12. JECFA (2008) Steviol glycosides. In: Summary and conclusions of the 69th meeting of the joint FAO/WHO expert committee on food additives (JECFA), Rome. http://www. faoorg/ag/agn/agns/files/jecfa69_finalpdf. Accessed 13 Jan 2010.
13. Ministère de l'Économie, de l'Industrie et de l'Émploi (2009) Arrêté due 26 août relatif à l'emploi du rébaudioside A comme additif alimentaire. J Officiel de la République française. Texte 6. http://www. Stevia-infofr/decret-autorisation_Stevia_aout2009pdf. Accessed 5 Jan 2010.
14. Kitada Y, Sasaki M, Yamazoe Y (1989) Simultaneous determination of stevioside, rebaudioside A and C and dulcoside A in foods by high-performance liquid chromatography. J chromatog 474: 447-451.
15. Bovanová L, Brandŝteterová E, Baxa S (1998) HPLC determination of stevioside in plant material and food samples. Z Lebensmittel Untersuch FA 207: 352-355.
16. Vaněk T, Nepovim A, Valiček P (2001) Determination of stevioside in plant material and fruit teas. J Food Comp Anal 14: 383-388.
17. Kolb N, Herrera DJ, Uliana RF (2001) Analysis of sweet diterpene glycosides from Stevia rebaudiana: improved HPLC method. J Agric Food Chem 49: 4538-4541.
18. Pól J et al (2007) Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana: methanol versus water. Anal Bioanal Chem 388: 1847-1857.
19. Choi HYH et al (2002) Supercritical fluid extraction and liquid chromatographic-electrospray mass spectrometric analysis of stevioside from Stevia rebaudiana leaves. Chromatographia 55: 617-620.
20. Pasquel A et al (2000) Extraction of Stevia glycosides with CO2 plus water CO2 plus ethanol and CO2 plus water plus ethanol. Braz J Chem Eng 17: 271-282.
21. Hutapea AM, Toskulkao C, Wilairat P, Buddhasukh D (1999) High-performance liquid chromatographic separation and quantification of stevioside and its metabolites. J Liq Chromatogr RT 22: 1161-1170.
22. Geuns JMC (2008) Analysis of steviol glycosides: validation of the methods. In: Proceedings of the 2nd Stevia symposium, Leuven, Belgium.
23. Chang SS, Cook JM (1983) Stability studies of stevioside and rebaudioside A in carbonated beverages. J Agric Food Chem 31: 409-412.
24. Richman A et al (2005) Functional genomics uncovers three glycosyltransferases involved in the synthesis of the major sweet glycosides of Stevia rebaudiana. Plant J 41: 56-67.
25. Pól J, Hohnová B, Hyötyläinen T (2007) Characterisation of Stevia rebaudiana by comprehensive two-dimensional liquid chromatography time-of-flight mass spectrometry. J Chromatog A 1150: 85-92.
26. Rajasekaran T et al (2008) Analysis of predominant steviosides in Stevia rebaudiana Bertoni by liquid chromatography/electrospray ionization-mass spectrometry. Food Biotechnol 22: 179-188.
27. Ahmed MJ, Smith RM (2002) Determination of stevioside by high-performance liquid chromatography with pulsed amperometric detection. J Sep Sci 25: 170-172.
28. Clos JF, DuBois GE, Prakash I (2008) Photostability of rebaudioside A and stevioside in beverages. J Agric Food Chem 56: 8507-8513.
29. Hemstrom P, Irgum J (2006) Hydrophilic interaction chromatography. J Sep Sci 29: 1784-1821.
30. Kienle U (1993) Einfluss von Bewässerung und Schnittfolge auf den Ertrag von Stevia rebaudiana in Südspanien. In: Göttinger Beiträge zur Land- und Forstwirtschaft in den Tropen und Subtropen, vol 84, pp 4-108.
31. Lankes C, Pude R (2008) Possibilities for growth of Stevia in European temperate zones. In: Proceedings of the 2nd Stevia symposium, Leuven.
32. Pude R, Schmitz-Eiberger M, Noga G (2005) Development yield and selected contents of Stevia rebaudiana. Z Arz Gew Pfla 10: 37-43.
33. Pude R (2005) Stevia-Anbau lohnt sich. Gemüse 10: 27-28.
34. Metivier J, Viana AM (1979) The effect of long and short day length upon growth of whole plants and the level of soluble proteins sugars and steviosides in leaves of Stevia rebaudiana Bert. J Exp Bot 30: 1211-1222.
35. Manara W, Veiga P, Tarrago MFS, Manara NFT (1986) Estévia- Stevia rebaudiana (Bert)Bertoni: Aspectos do cultivo no Rio Grande do Sul. Santa Maria: Universidade Federal de Santa Maria RS Centro de Ciências Rurais-Ministério da Educacao, Santa Maria, Brasilia.
36. Shock CC (1982) Experimental cultivation of Rebaudi′s Stevia in California. Agron Prog Rep 122.
37. Brandle JE, Rosa N (1992) Heritability for yield leaf-stem ratio and stevioside content estimated from a landrace cultivar of Stevia rebaudiana. Can J Plant Sci 72: 1263-1266.
38. Megeji NW, Kumar JK, Singh V, Kaul VK, Ahuja PS (2005) Introducing Stevia rebaudiana a natural zero-calorie sweetener. Cur Sci India 88: 31-35.