Effects of prolonged formalin fixation on the immunohistochemical detection of infectious agents in formalin-fixed, paraffin-embedded tissues

Veterinary Pathology

Volumn 47, Issue 3, 2010, Pages 529-535

  Webster, J D ^a   Miller, M A ^a   DuSold, D ^a   Ramos Vara, José ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Domestic animals; Fixation; Formalin; Immunohistochemistry; Infectious

Indexed keywords

BACTERIAL ANTIGEN; FIXATIVE; FORMALDEHYDE; PARAFFIN; VIRUS ANTIGEN;

ANIMAL; ANIMAL DISEASE; ARTICLE; CAT; CATTLE; CHEMISTRY; COMMUNICABLE DISEASE; DOG; IMMUNOHISTOCHEMISTRY;

ANIMALS; ANTIGENS, BACTERIAL; ANTIGENS, VIRAL; CATS; CATTLE; COMMUNICABLE DISEASES; DOGS; FIXATIVES; FORMALDEHYDE; IMMUNOHISTOCHEMISTRY; PARAFFIN EMBEDDING;

ANIMALIA; BARTONELLA HENSELAE; BOVINE RESPIRATORY SYNCYTIAL VIRUS;

EID: 77954531941     PISSN: 03009858     EISSN: 15442217     Source Type: Journal    
DOI: 10.1177/0300985809359607     Document Type: Article

References (21)

1. Arnold MM, Srivastava S., Fredenburgh J., Stockard CR, Myers RB, Grizzle WE: Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens. Biotech Histochem 71:224-230, 1996.
2. Battifora H., Kopinski M.: The influence of protease digestion and duration of fixation on the immunostaining of keratins: a comparison of formalin and ethanol fixation. J Histochem Cytochem 34:1095-1100, 1986.
3. Boenisch T.: Effect of heat-induced antigen retrieval following inconsistent formalin fixation. Appl Immunohistochem Mol Morphol 13:283-286, 2005.
4. Del Piero F., Wilkins PA, Dubovi EJ, Biolatti B., Cantile C.: Clinical, pathologic, immunohistochemical, and virologic findings of eastern equine encephalomyelitis in two horses. Vet Pathol 38:451-456, 2001.
5. Eltoum I., Fredenburgh J., Myers RB, Grizzle WE: Introduction to the theory and practice of fixation of tissues. J Histotechnol 24:173-190, 2001.
6. Fox CH, Johnson FB, Whiting J., Roller PP: Formaldehyde fixation. J Histochem Cytochem 33:845-853, 1985.
7. Johnson R., Ramos-Vara J., Vemulapalli R.: Identification of Bartonella henselae in an aborted equine fetus. Vet Pathol 46:277-281, 2009.
8. Miller MA, Ramos-Vara JA, Kleiboeker SB, Larson RL: Effects of delayed or prolonged fixation on immunohistochemical detection of bovine viral diarrhea virus type I in skin of two persistently infected calves. J Vet Diagn Invest 17:461-463, 2005.
9. Opriessnig T., Meng XJ, Halbur PG: Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest 19:591-615, 2007.
10. Ramos-Vara JA: Technical aspects of immunohistochemistry. Vet Pathol 42:405-426, 2005.
11. Ramos-Vara JA, Beissenherz ME: Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed paraffin-embedded tissues: experience with 63 markers. J Vet Diagn Invest 12:307-311, 2000.
12. Ramos-Vara JA, Beissenherz ME, Miller MA, Johnson GC, Pace LW, Fard A., Kottler SJ: Retrospective study of 338 canine oral melanomas with clinical, histologic, and immunohistochemical review of 129 cases. Vet Pathol 37:597-608, 2000.
13. Ramos-Vara JA, Kiupel M., Baszler T., Bliven L., Brodersen B., Chelack B., Czub S., Del Piero F., Dial S., Ehrhart EJ, Graham T., Manning L., Paulsen D., Valli VE, West K.: Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. J Vet Diagn Invest 20:393-413, 2008.
14. Ramos-Vara JA, Miller MA, Boucher M., Roudabush A., Johnson GC: Immunohistochemical detection of uroplakin III, cytokeratin 7, and cytokeratin 20 in canine urothelial tumors. Vet Pathol 40:55-62, 2003.
15. Ramos-Vara JA, Miller MA, Johnson GC: Immunohistochemical characterization of canine hyperplastic hepatic lesions and hepatocellular and biliary neoplasms with monoclonal antibody hepatocyte paraffin 1 and a monoclonal antibody to cytokeratin 7. Vet Pathol 38:636-643, 2001.
16. Ramos-Vara JA, Miller MA, Johnson GC, Pace LW: Immunohistochemical detection of thyroid transcription factor-1, thyroglobulin, and calcitonin in canine normal, hyperplastic, and neoplastic thyroid gland. Vet Pathol 39:480-487, 2002.
17. Rossow KD, Benfield DA, Goyal SM, Nelson EA, Christopher-Hennings J., Collins JE: Chronological immunohistochemical detection and localization of porcine reproductive and respiratory syndrome virus in gnotobiotic pigs. Vet Pathol 33:551-556, 1996.
18. Shi SR, Cote RJ, Chaiwun B., Young LL, Shi Y., Hawes D., Chen T., Taylor CR: Standardization of immunohistochemistry based on antigen retrieval technique for routine formalin-fixed tissue sections. Appl Immunohistochem Mol Morphol 6:89-96, 1998.
19. Van Alstine WG, Popielarczyk M., Albregts SR: Effect of formalin fixation on the immunohistochemical detection of PRRS virus antigen in experimentally and naturally infected pigs. J Vet Diagn Invest 14:504-507, 2002.
20. Webster JD, Miller MA, Dusold D., Ramos-Vara J.: Effects of prolonged formalin-fixation on diagnostic immunohistochemistry in domestic animals. J Histochem Cytochem 57:753-761, 2009.
21. Werner M., Chott A., Fabiano A., Battifora H.: Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol 24:1016-1019, 2000.