The paradoxical stimulation by a reversible thrombin inhibitor of thrombin generation in plasma measured with thrombinography is caused by α2-macroglobulin-thrombin

Journal of Thrombosis and Haemostasis

Volumn 8, Issue 6, 2010, Pages 1281-1289

  Wagenvoord, R J ^a   Deinum, J ^b   Elg, M ^b   Hemker, H C ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b ASTRAZENECA R AND D   (Sweden)

Author keywords

Calibrated automated thrombinography; Reversible direct thrombin inhibitors; Sub sampling technique; Thrombin generation

Indexed keywords

ALPHA 2 MACROGLOBULIN; AR H 067637; THROMBIN; THROMBIN INHIBITOR; UNCLASSIFIED DRUG;

ALGORITHM; ARTICLE; DISSOCIATION CONSTANT; HUMAN; MICHAELIS CONSTANT; PRIORITY JOURNAL; STIMULATION;

ALPHA-MACROGLOBULINS; ANTITHROMBINS; CALIBRATION; HUMANS; THROMBIN;

EID: 77954497588     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/j.1538-7836.2010.03822.x     Document Type: Article

References (22)

1. Crawley JT, Zanardelli S, Chion CK, Lane DA. The central role of thrombin in hemostasis. J Thromb Haemost 2007, 5(Suppl. 1):95-101.
2. Di Cera E. Thrombin as procoagulant and anticoagulant. J Thromb Haemost 2007, 5(Suppl. 1):196-202.
3. Lane DA, Philippou H, Huntington JA. Directing thrombin. Blood 2005, 106:2605-12.
4. Hemker HC, Wielders S, Kessels H, Beguin S. Continuous registration of thrombin generation in plasma, its use for the determination of the thrombin potential. Thromb Haemost 1993, 70:617-24.
5. Hemker HC, Giesen P, AlDieri R, Regnault V, de Smed E, Wagenvoord R, Lecompte T, Beguin S. The calibrated automated thrombogram (CAT): a universal routine test for hyper- and hypocoagulability. Pathophysiol Haemost Thromb 2002, 32:249-53.
6. Hemker HC, Giesen P, Al Dieri R, Regnault V, de Smedt E, Wagenvoord R, Lecompte T, Beguin S. Calibrated automated thrombin generation measurement in clotting plasma. Pathophysiol Haemost Thromb 2003, 33:4-15.
7. Hemker HC, Giesen PL, Ramjee M, Wagenvoord R, Beguin S. The thrombogram: monitoring thrombin generation in platelet-rich plasma. Thromb Haemost 2000, 83:589-91.
8. Hron G, Kollars M, Binder BR, Eichinger S, Kyrle PA. Identification of patients at low risk for recurrent venous thromboembolism by measuring thrombin generation. JAMA 2006, 296:397-402.
9. van Hylckama Vlieg A, Christiansen SC, Luddington R, Cannegieter SC, Rosendaal FR, Baglin TP. Elevated endogenous thrombin potential is associated with an increased risk of a first deep venous thrombosis but not with the risk of recurrence. Br J Haematol 2007, 138:769-74.
10. Dargaud Y, Beguin S, Lienhart A, Al Dieri R, Trzeciak C, Bordet JC, Hemker HC, Negrier C. Evaluation of thrombin generating capacity in plasma from patients with haemophilia A and B. Thromb Haemost 2005, 93:475-80.
11. Dargaud Y, Lienhart A, Meunier S, Hequet O, Chavanne H, Chamouard V, Marin S, Negrier C. Major surgery in a severe haemophilia A patient with high titre inhibitor: use of the thrombin generation test in the therapeutic decision. Haemophilia 2005, 11:552-8.
12. Deinum J, Elg M. The interaction between human thrombin and antithrombin studied by surface plasmon resonance, and the effect of melagatran. Spectroscopy 2002, 16:257-70.
13. Wagenvoord RJ, Deinum J, Elg M, Hemker C. The effect of direct thrombin inhibitors (DTIs) in clotting plasma. J Thromb Haemost 2007, 5(Suppl. 2).
14. Beguin S, Lindhout T, Hemker HC. The mode of action of heparin in plasma. Thromb Haemost 1988, 60:457-62.
15. Deinum J, Mattsson C, Inghardt T, Elg M. Biochemical and pharmacological effects of the direct thrombin inhibitor AR-H067637. Thromb Haemost 2009, 101:1051-9.
16. Rijkers DT, Wielders SJ, Beguin S, Hemker HC. Prevention of the influence of fibrin and alpha2-macroglobulin in the continuous measurement of the thrombin potential: implications for an endpoint determination of the optical density. Thromb Res 1998, 89:161-9.
17. Thaler E, Schmer G. A simple two-step isolation procedure for human and bovine antithrombin II/III (heparin cofactor): a comparison of two methods. Br J Haematol 1975, 31:233-43.
18. Bajaj SP, Rapaport SI, Prodanos C. A simplified procedure for purification of human prothrombin, factor IX and factor X. Prep Biochem 1981, 11:397-412.
19. Lindhout T, Govers-Riemslag JW, van de Waart P, Hemker HC, Rosing J. Factor Va-factor Xa interaction. Effects of phospholipid vesicles of varying composition. Biochemistry 1982, 21:5494-502.
20. Barrett AJ. Alpha 2-macroglobulin. Methods Enzymol 1981, 80:737-54.
21. Coen Hemker H, Hemker PW, Al Dieri R. The technique of measuring thrombin generation with fluorescent substrates: 4. The H-transform, a mathematical procedure to obtain thrombin concentrations without external calibration. Thromb Haemost 2009, 101:171-7.
22. Wagenvoord R, Hemker PW, Hemker HC. The limits of simulation of the clotting system. J Thromb Haemost 2006, 4:1331-8.