Ethyl acetate fraction of the root of Rubia cordifolia L. inhibits keratinocyte proliferation in vitro and promotes keratinocyte differentiation in vivo: Potential application for psoriasis treatment

Phytotherapy Research

Volumn 24, Issue 7, 2010, Pages 1056-1064

  Lin, Z X ^a   Jiao, B W ^a   Che, C T ^a   Zuo, Z ^a   Mok, C F ^a   Zhao, M ^a   Ho, W K K ^a   Tse, W P ^a   Lam, K Y ^a   Fan, R Q ^b   Yang, Z J ^c   Cheng, C H K ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

^b GUANGDONG PROVINCIAL HOSPITAL OF CHINESE MEDICINE   (China)

^c HONG KONG BAPTIST UNIVERSITY   (Hong Kong)

Author keywords

Apoptosis; Differentiation; HaCaT keratinocytes; Mouse tail test; Proliferation; Psoriasis; Rubia cordifolia L.

Indexed keywords

ACETIC ACID ETHYL ESTER; BUTANOL; PLANT EXTRACT; RUBIA CORDIFOLIA EXTRACT; UNCLASSIFIED DRUG; WATER;

ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DOSE TIME EFFECT RELATION; DRUG POTENCY; EPIDERMIS; GEL; HUMAN; HUMAN TISSUE; IN VITRO STUDY; IN VIVO STUDY; KERATINOCYTE; MALE; MITOCHONDRIAL MEMBRANE POTENTIAL; MOUSE; NONHUMAN; OUTCOME ASSESSMENT; PLANT ROOT; PSORIASIS; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RUBIA; STANDARDIZATION; THICKNESS; TREATMENT DURATION; TREATMENT OUTCOME;

ACETATES; ADMINISTRATION, CUTANEOUS; ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; DERMATOLOGIC AGENTS; HUMANS; KERATINOCYTES; MALE; MICE; MICE, INBRED BALB C; PLANT EXTRACTS; PLANT ROOTS; PSORIASIS; RUBIA;

RUBIA CORDIFOLIA;

EID: 77954484937     PISSN: 0951418X     EISSN: 10991573     Source Type: Journal    
DOI: 10.1002/ptr.3079     Document Type: Article

References (22)

1. Abrams JM, White K, Fessler LI, Steller H. 1993. Programmed cell death during Drosophila embryogenesis. Development 117: 29-43. (Pubitemid 23094155)
2. Bosman B. 1994. Testing of lipoxygenase inhibitors, cyclooxygenase inhibitors, drugs with immunomodulating properties and some reference antipsoriatic drugs in the modified mouse tail test, an animal model of psoriasis. Skin Pharmacol 7: 324-334. (Pubitemid 24202329)
3. Bosman B, Matthiesen T, Hess V, Friderichs E. 1992. A quantitative method for measuring antipsoriatic activity of drugs by the mouse tail test. Skin Pharmacol 5: 41-48.
4. Chinese Pharmacopoeia Committee. 2005. Chinese Pharmacopoeia. Chemical Industrial Press: Beijing, China; 236.
5. Du Vivier A, Stoughton B. 1975. An animal model for screening topical and systemic drugs for potential use in the treatment of psoriasis. J Invest Dermatol 65: 235-237.
6. Ernst JD, Yang L, Rosales JL, Broaddust VC. 1998. Preparation and characterization of an endogenously fluorescent annexin for detection of apoptotic cells. Anal Biochem 260: 18-23.
7. Hofbauer M, Dowd PM, Atkinson J, Whitefield M, Greaves MW. 1988. Evaluation of a therapeutic concentration of dithranol in the mouse tail test. Br J Dermatol 118: 85-89.
8. Jarrett A, Spearman RIG. 1964. Psoriasis. In Histochemistry of the Skin. Taverner D, Trounce J (eds). University Press: London.
9. Jia HS, Wu XX. 2002. The progress of clinical studies on Chinese herbal medicine for Psoriasis. Chin J Info Trad Chin Med 9: 74-76.
10. Lebwohl M. 2003. Psoriasis. Lancet 361: 1197-1204.
11. Mosmann TR. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.
12. Nickoloff BJ, Nestle FO. 2004. Recent insights into the immunopathogenesis of psoriasis provide new therapeutic opportunities. J Clin Invest 113: 1664-1675.
13. Pol A, Bergers M, Schalkwijk J. 2003. Comparison of antiproliferative effects of experimental and established antipsoriatic drugs on human keratinocytes, using a simple 96-well-plate assay. In Vitro Cell Dev Biol- Animal 39: 36-42.
14. Sebok B, Bonnekoh B, Kerenyi M, Gollnick H. 2000. Tazarotene induces epidermal cell differentiation in the mouse tail test used as an animal model for psoriasis. Skin Pharmacol Applied Skin Physiol 13: 285-291.
15. Sebok B, Szabados T, Kerenyi M, Schneider I, Mahrle G. 1996. Effect of fumaricacid, its dimethylester and topical antipsoriatic drugs on epidermal differentiation in the mouse tail model. Skin Pharmacol 9: 99-103. (Pubitemid 26109077)
16. Takahashi H and Iizuka H. 2004. Treatment of the keratinizing skin disorder, psoriasis, by topical vitamin D3. Clin Calcium 10: 129-132.
17. Tse WP, Che CT, Liu K, Lin ZX. 2006. Evaluation of the antiproliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cells. J Ethnopharmacol 108: 133-141.
18. Tse WP, Cheng CHK, Che CT, Zhao M, Lin ZX. 2007. Induction of apoptosis underlies the Radix Rubiae-mediated antiproliferative action on human epidermal keratinocytes: Implications for psoriasis treatment. Int J Mol Med 20: 663-672. 1
19. Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C. 1995. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184: 39-51.
20. Wolf R, Shechter H, Brenner S. 1995. Research models for psoriasis: 'Great Expectations'. Int J Dermatol 34: 448-455.
21. Wrench R, Britten A. 1975. Evaluation of coal tar fractions for use in psoriasiform diseases using the mouse tail test. (I) High and low temperature tars and their constituents. Br J Dermatol 92: 569-574.
22. Zhang H, Qu X. 2002. Advances in experimental studies on treatment of psoriasis by traditional Chinese medicine. J Trad Chin Med 22: 61-66. (Pubitemid 41238520)