Evaluation of green fluorescent protein as a reporter gene and phosphinothricin as the selective agent for achieving a higher recovery of transformants in cucumber (Cucumis sativus L. cv. Poinsett76) via Agrobacterium tumefaciens

In Vitro Cellular and Developmental Biology - Plant

Volumn 46, Issue 4, 2010, Pages 329-337

  Selvaraj, N ^b   Kasthurirengan, S ^a   Vasudevan, A ^c   Manickavasagam, M ^a   Choi, C W ^d   Ganapathi, A ^a  

^a BHARATHIDASAN UNIVERSITY   (India)

^b Periyar EVR College Autonomous   (India)

^c Pusan National University   (South Korea)

^d PaiChai University   (South Korea)

Author keywords

Agrobacterium tumefaciens; Cotyledons; Cucumber; Green fluorescent protein (GFP); Phosphinothricin; Transformation efficiency

Indexed keywords

AGROBACTERIUM TUMEFACIENS; CUCUMIS SATIVUS;

EID: 77954458141     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-010-9288-5     Document Type: Article

References (39)

1. Anonymous (2005) Food and Agriculture Organization of the United Nations, Statistics Division. In: http://faostat. fao. org.
2. Baranski R.; Klocke E.; Schumann G. Green fluorescent protein as an efficient selection marker for Agrobacterium rhizogenes mediated carrot transformation. Plant Cell Rep 25: 190-197; 2006. doi: 10. 1007/s00299-005-0040-2.
3. Chalfie M.; Tu Y.; Euskirehen G.; Ward W. W.; Prasher D. C. Green fluorescent protein as marker for gene expression. Science 263: 802-805; 1994. doi: 10. 1126/science. 8303295.
4. Chee P. P. Transformation of Cucumis sativus tissue by Agrobacterium tumefaciens and the regulations of transformed plants. Plant Cell Rep 9: 245-248; 1990. doi: 10. 1007/BF00232293.
5. Chee P. P.; Slightom J. L. Transfer and expression of Cucumber Mosaic Virus Coat protein gene in the genome of Cucumis sativus. J Amer Soc Sci Hortic 116: 1098-1102; 1991.
6. Chilton M. D.; Currier T. C.; Farrand S. K.; Bendich A. J.; Gordon M. P.; Nester E. W. Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc. Natl. Acad. Sci. 71: 3672-3676; 1974. doi: 10. 1073/pnas. 71. 9. 3672.
7. Chiu W. L.; Niwa Y.; Zeng W.; Hirano T.; Kobayashi H.; Sheen J. Engineered GFP as a vital reporter in plants. Curr Biol 6: 325-330; 1996. doi: 10. 1016/S0960-9822(02)00483-9.
8. Cho H. J.; Widholm J. M. Agrobacterium tumefaciens-mediated transformation of the legume Astragalus sinicus using kanamycin resistance selection and green fluorescent protein expression. Plant Cell Tissue Organ Cult 69: 251-258; 2002. doi: 10. 1023/A: 1015668132503.
9. Clemente R. M. P.; Sanjuan A. P.; Ferriz L. G.; Beltran J. P.; Canas L. A. Transgenic peach plants (Prunus persica L.) produced by genetic transformation of embryo sections using the green fluorescent protein (GFP) as an in vivo marker. Mol Breed 14: 419-427; 2005. doi: 10. 1007/s11032-005-0506-5.
10. Dellaporta S. C.; Wood J.; Hicks J. B. A plant DNA mini preparation, version II. Plant Mol Biol Report 1: 19-21; 1983. doi: 10. 1007/BF02712670.
11. Gaba V.; Feldmesser E.; Gal-On A.; Kles H.; Antignus Y. Genetic transformation of a recalcitrant melon (Cucumis melo L.) variety. In: Lester G.; Dunlop J. (eds) Cucurbitaceae 94. Gateway, Edinburg, pp 188-190; 1995.
12. Gambley R. L.; Dodd W. A. An in vitro technique for production of de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.). Plant Cell Tissue Organ Cult 20: 177-183; 1990. doi: 10. 1007/BF00041879.
13. George E. F. Plant propagation by tissue culture-part 2: The technology, Exegetics Limited. Edington, Wilts, England: 50; 1996.
14. Ghorbel R.; Juarez J.; Navarro L.; Pena L. Green fluorescent protein as a screenable marker to increase the efficiency of generating transgenic woody fruit plants. Theor Appl Genet 99: 350-358; 1999. doi: 10. 1007/s001220051244.
15. Halfhill M. D.; Richards H. A.; Mabon S. A.; Stewart C. N. Expression of GFP and Bt transgene in Brassica napus and hybridization and introgression with Brassica rapa. Theor Appl Genet 103: 659-667; 2001. doi: 10. 1007/s001220100613.
16. Jefferson R. A.; Kavanagh T. A.; Bevan M. W. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907; 1987.
17. Kaeppler H. F.; Carlson A. R.; Menon G. K. Routine utilization of green fluorescent protein as a visual selectable marker for cereal transformation. In Vitro Cell Dev Biol-Plant 37: 120-126; 2001. doi: 10. 1007/s11627-001-0023-0.
18. Molinier J.; Himber C.; Hahne G. Use of green fluorescent protein for detection of transformed shoots and homozygous offspring. Plant Cell Rep 19: 219-223; 2000. doi: 10. 1007/s002990050002.
19. Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473-497; 1962. doi: 10. 1111/j. 1399-3054. 1962. tb08052. x.
20. Muruganantham M.; Amutha S.; Selvaraj N.; Vengadesan G.; Ganapathi A. Efficient Agrobacterium-mediated transformation of Vigna mungo using immature cotyledonary-node explants and phosphinothricin as the selection agent. In Vitro Cell Dev Biol 43: 550-557; 2007. doi: 10. 1007/s11627-007-9060-7.
21. Nishibayashi S.; Kaneko H.; Hayakawa T. Transformation of cucumber (Cucumis sativus L.) plants using Agrobacterium tumefaciens and regeneration from hypocotyl explants. Plant Cell Rep 15: 809-814; 1996. doi: 10. 1007/BF00233145.
22. Oridate T.; Atsumi H.; Ito S.; Araki H. Genetic difference in somatic embryogenesis from seed in melon (Cucumis melo L.). Plant Cell Tissue Organ Cult 29: 27-30; 1992. doi: 10. 1007/BF00036142.
23. Pigeaire A.; Abernethy D.; Smith P. M.; Simpson K.; Fletcher N.; Lu C. Y.; Atkins C. A.; Cornish E. Transformation of a grain legume (Lupinus augustifolius L.) via Agrobacterium tumefaciens-mediated gene transfer to shoot apices. Mol Breed 3: 341-349; 1991. doi: 10. 1023/A: 1009642620907.
24. Rajagopalan P. A.; Perl-Treves R. Improved cucumber transformation by a modified explant dissection and selection protocol. Hortic Sci 40: 431-435; 2005.
25. Raharjo S. H. T.; Hernandez M. O.; Zhang Y. Y.; Punja Z. K. Transformation of pickling cucumber with chitinase-encoding genes using Agrobacterium tumefaciens. Plant Cell Rep 15: 591-596; 1996. doi: 10. 1007/BF00232459.
26. Sambrook J.; Fritsch E. F.; Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Woodbury; 1989.
27. Sapountzakis G.; Tsaftaris A. S. Transfer and expression of the firefly luciferase gene in cucumber. Cucurbit Genet Coop Rep 19: 38-41; 1996.
28. Sarmento G. G.; Alpert F. A.; Tang S.; Punja Z. K. Factors influencing Agrobacterium tumefaciens mediated transformation and expression of kanamycin resistance in pickling cucumber. Plant Cell Tissue Organ Cult 31: 185-193; 1992. doi: 10. 1007/BF00036222.
29. Schulze J.; Balko C.; Zellner B.; Koprek T.; Hansch R.; Nerlich A.; Mendel R. R. Biolistic transformation of cucumber using embryogenic suspension cultures: long-term expression of reporter genes. Plant Sci 112: 197-206; 1995. doi: 10. 1016/0168-9452(95)04261-X.
30. Selvaraj N.; Vasudevan A.; Manickavasagam M.; Kasthurirengan S.; Ganapathi A. High frequency shoot regeneration from cotyledon explants of cucumber via organogenesis. Sci Hortic-Amsterdam 112: 2-8; 2007. doi: 10. 1016/j. scienta. 2006. 12. 037.
31. Stewart Jr. C. N. The utility of green fluorescent protein in transgenic plants. Plant Cell Rep 20: 376-382; 2001. doi: 10. 1007/s002990100346.
32. Tabei Y.; Kitade S.; Nisihizawa Y.; Kikuchi N.; Kayano T.; Hibi T.; Akutsu K. Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea). Plant Cell Rep 17: 159-164; 1998. doi: 10. 1007/s002990050371.
33. Tabei Y.; Nishio T.; Kusihara K.; Kanno T. Selection of transformed callus in a liquid medium and regeneration of transgenic plants in cucumber (Cucumis sativus L.). Breed Sci 44: 47-51; 1994.
34. Trulson A. J.; Simpson R. B.; Shahin E. A. Transformation of cucumber (Cucumis sativus L.) plants with Agrobacterium rhizogenes. Theor Appl Genet 73: 11-15; 1986. doi: 10. 1007/BF00273711.
35. Vengadesan G.; Prem Anand R.; Selvaraj N.; Perl-Treves R.; Ganapathi A. Transfer and expression of nptII and bar genes in cucumber (Cucumis sativus L.). In Vitro Cell Dev Biol 41: 17-21; 2005. doi: 10. 1079/IVP2004602.
36. Wehner T. C.; Cade R. M.; Locy R. D. Cell, tissue and organ culture techniques for genetic improvement of cucurbits. In: Bates D. M.; Robinson R. M.; Jeffrey C. (eds) Biology and Utilization of the Cucurbitaceae. Cornell University, Ithaca; 1990.
37. Yin Z.; Bartoszewski G.; Szwacka M.; Malepszy S. Cucumber transformation methods-the review. Biotech 1: 95-113; 2005a.
38. Yin Z.; Pla{ogonek}der W.; Wiśniewska A.; Szwacka M.; Malepszy S. Transgenic cucumber-a current state. Folia. Hortic 17/1: 73-90; 2005b.
39. Zhang M.; Cui H.; Wang B.; Jia S. Stimulatory effects of different cytokinin on direct plant regeneration from cotyledon explants in Cucumis sativus L. Cucurbit Genetic Coop Rep 24: 13-16; 2001.