Evaluation of COBAS TaqMan real time PCR assay for the diagnosis of Mycobacterium tuberculosis

Indian Journal of Medical Research

Volumn 132, Issue 7, 2010, Pages 100-102

  Chandran, Salesh P ^a   Kenneth, John ^a  

^a ST JOHN'S NATIONAL ACADEMY OF HEALTH SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL GROWTH; BACTERIUM CULTURE; GENE TARGETING; HUMAN; LETTER; LUNG TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; REAL TIME POLYMERASE CHAIN REACTION; ANALYTICAL EQUIPMENT; BACTERIAL COUNT; COMPARATIVE STUDY; EVALUATION; GENETICS; INDIA; METHODOLOGY; MICROBIOLOGY; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION; TUBERCULOSIS;

COLONY COUNT, MICROBIAL; HUMANS; INDIA; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; TUBERCULOSIS;

EID: 77954454254     PISSN: 09715916     EISSN: 09715916     Source Type: Journal    
DOI: None     Document Type: Letter

References (17)

1. Centers for Disease Control and Prevention. Laboratory Manual for acid-fast microscopy. U.S. CDC, Atlanta, Georgia, USA: Department of Health, Education and Welfare; 1976.
2. Levin W, Branon GR, McMillen S. The culture method of laboratory diagnosis of tuberculosis. Am J Public Health 1950; 40: 1305-10.
3. Laraque F, Griggs A, Slopen M, Munsiff SS. Performance of nucleic acid amplifcation tests for diagnosis of tuberculosis in a large urban setting. Clin Infect Dis 2009; 49: 46-54.
4. Lipsky BA, Gates J, Tenover FC, Plorde JJ. Factors affecting the clinical value of microscopy for acid-fast bacilli. Rev Infect Dis 1984; 6: 214-22.
5. Gamboa F, Fernandez G, Padilla E, Manterola JM, Lonca J, Cardona PJ, et al. Comparative evaluation of initial and new versions of the Gen-Probe Amplifed Mycobacterium Tuberculosis Direct Test for direct detection of Mycobacterium tuberculosis in respiratory and nonrespiratory specimens. J Clin Microbiol 1998; 36: 684-9.
6. Eing BR, Becker A, Sohns A, Ringelmann R. Comparison of Roche Cobas Amplicor Mycobacterium tuberculosis Assay with In-House PCR and culture for detection of M. tuberculosis. J Clin Microbiol 1998; 36: 2023-9.
7. Sjobring U, Mecklenburg M, Andersen AB, Miorner H. Polymerase chain reaction for detection of Mycobacterium tuberculosis. J Clin Microbiol 1990; 28: 2200-4.
8. Kent PT, Kubica GP. Public health mycobacteriology: a guide for the level III laboratory. Atlanta, GA, USA: Department of Health and Human Services, Centers for Disease Control; 1985.
9. Srisuwanvilai LO, Monkongdee P, Podewils LJ, Ngamlert K, Pobkeeree V, Puripokai P. Performance of the BACTEC MGIT 960 compared with solid media for detection of Mycobacterium in Bangkok, Thailand. Diagn Microbiol Infect Dis 2008; 61: 402-7.
10. Bodmer T, Gurtner A, Scholkmann M, Matter L. Evaluation of the COBAS AMPLICOR MTB system. J Clin Microbiol 1997; 35: 1604-5.
11. ® MTB test. Roche Molecular Systems. Branchburg, NJ, USA; 2007.
12. Becton, Dickinson. BACTEC MGIT 960 System User's Manual. 2004/06. Becton, Dickinson and Company, Sparks, Maryland, USA.
13. Alcaide F, Benítez MA, Escribà JM, Martín R. Evaluation of the BACTEC MGIT 960 and the MB/BacT systems for recovery of Mycobacteria from clinical specimens and for species identifcation by DNA AccuProbe. J Clin Microbiol 2000; 38: 398-401.
14. Altman D, Bland J. Statistics notes: diagnostic tests 1: sensitivity and specifcity. Br Med J 1994; 308: 1552.
15. Altman D, Bland J. Statistics notes: diagnostic tests 2: predictive values. Br Med J 1994; 309: 102.
16. Higurashi Y, Satoh T, Hirai T, Moriya K, Koike K. The fundamental evaluation of COBAS TaqMan48 using clinical specimens. Kekkaku 2009; 84: 117-24.
17. Broccolo F, Scarpellini P, Locatelli G, Zingale A, Brambilla AM, Cichero P, et al. Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays. J Clin Microbiol 2003; 41: 4565-72.