Oral epithelial atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, peppers and hot meals, using the AgNOR and Papanicolaou staining techniques

Diagnostic Cytopathology

Volumn 38, Issue 7, 2010, Pages 489-495

  Ahmed, Hussain Gadelkarim ^a   Ali Ebnoof, Syda Omer M ^a   Hussein, Mohmmed Omer M ^b   Gbreel, Afra Yousif A ^b  

^a UNIVERSITY OF KHARTOUM   (Sudan)

^b Sudanese Cancer Society   (Sudan)

Author keywords

Alcohol; Hot meal; Oral epithelial; Peppers; Smoking

Indexed keywords

SILVER DERIVATIVE;

ADULT; ALCOHOL CONSUMPTION; ARTICLE; CELL PROLIFERATION; CELL TRANSFORMATION; CHROMOSOME NOR; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOLOGY; FEMALE; HOT MEAL; HUMAN; HUMAN TISSUE; KERATINIZATION; MALE; MEAL; MOUTH CANCER; MOUTH MUCOSA; PAPANICOLAOU TEST; PEPPER; PRIORITY JOURNAL; SCREENING; SILVER STAINING; SMOKING; SQUAMOUS CELL CARCINOMA; STAINING; VIRUS INFECTION;

ADOLESCENT; ADULT; ALCOHOL DRINKING; CANDIDA; CAPSICUM; FEMALE; FOOD; HEALTH; HOT TEMPERATURE; HUMANS; KERATINS; MALE; MIDDLE AGED; MOUTH MUCOSA; NUCLEOLUS ORGANIZER REGION; SILVER; SMOKING; STAINING AND LABELING; YOUNG ADULT;

EID: 77954414238     PISSN: 87551039     EISSN: 10970339     Source Type: Journal    
DOI: 10.1002/dc.21224     Document Type: Article

References (32)

1. Mayne ST, Morse DE, Winn DM. Schottenfeld D, Fraumeni JF, editors. Cancers of the oral cavity and pharynx. Cancer epidemiology and prevention, New York: Oxford University Press; 2006. p 674-696.
2. Pindborg JR, Smith CJ, Van Der Waal I. Histological typing of cancer and precancer of the oral mucosa. New York: Springer; 1997.
3. Douglas E, Morse WJP, Cleveland Deborah, et al. Smoking and drinking in relation to oral cancer and oral epithelial dysplasia. Cancer Causes Control 2007;18:919-929.
4. Castellsague X, Quintana MJ, Martinez MC, et al. The role of type of tobacco and type of alcoholic beverage in oral carcinogenesis. Int J Cancer 2004;108:741-749.
5. Blot WM, Laughlin JK, WinnMc DM, et al. Smoking and drinking in relation to oral and pharyngeal cancer. Cancer Res 1988;48: 3282-3287.
6. Jones AC, Pink FE, Sandow PL, Stewart CM, Migliorati CA, Baughman RA. The Cytobrush Plus cell collector in oral cytology. Oral Surg Oral Med Oral Pathol 1994;77:95-99.
7. Rick GM, Slater L. Oral brush biopsy: the problem of false positives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;96: 252.
8. Potter TJ, Summerlin DJ, Campbell JH. Oral malignancies associated with negative transepithelial brush biopsy. J Oral Maxillofac Surg 2003;61:674-677.
9. Frist SF. The oral brush biopsy: separating fact from fiction. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;96:654-656.
10. Ahmed HG, Idris AM, Ibrahim SO. Study of oral epithelial atypia among Sudanese tobacco users by exfoliative cytology. Anticancer Res 2003;23:1943-1949.
11. Costa Ade L, De Araújo NS, Pinto D dos S, De Araújo VC. PCNA/ AgNOR and Ki-67/AgNOR double staining in oral squamous cell carcinoma. J Oral Pathol Med 1999;28:438-441.
12. Schliephake H. Prognostic relevance of molecular markers of oral cancer - A review. J Oral Maxillofac Surg 2003;32:233-245.
13. Paiva RL, Sant'ana Filho M, Bohrer PL, Lauxen Ida S, Rados PV. AgNOR quantification in cells of normal oral mucosa exposed to smoking and alcohol. A cytopathologic study. Anal Quant Cytol Histol 2004;26:175-180.
14. Bancroft JD, Gamble M. Theory and Practice of Histochemical Techniques. 5th ed, London: Churchill Livingstone; 2002.
15. Ploton D, Menager M, Jeannesson P, Himber G, Pigeon F, Adnet JJ. Improvement in the staining and in the visualization of the argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J 1986;18:5-14. (Pubitemid 16108305)
16. Crocker J, Boldy DA, Egan MJ. How should we count AgNORS? Proposals for a standardized approach. J Pathol 1989;158:185-188. (Pubitemid 19185434)
17. Ogden GR, Cowpe JG, Chisholm DM. Cost of oral screening. Lancet 1991;337:920-921.
18. Fraga-Fernandez J, Vicandi-Plaza B. Diagnosis of hairy leukoplakia by exfoliative cytologic methods. Am J Clin Pathol 1992;97:262-266.
19. Orellana-Bustos Aiz, Ortega-Pinto AV. Evaluation of keratinization and AgNORs count in exfoliative cytology of normal oral mucosa from smokers and non-smokers. Med Oral 2004;9:197-203.
20. Ielmini MV, Heber E, Schwint AE, Cabrini RL, Itoiz ME. AgNOR are sensitive markers of radiation lesions in squamous epithelia. J Dent Res 2000;79:850-856.
21. Einstein TB, Sivapathasundharam B. Cytomorpho metric analysis of the buccal mucosa of tobacco users. Indian J Dent Res 2005;16:42-46.
22. Schwartz JL, Muscat JE, Baker V, et al. Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation, and apoptosis shows differences between smokers and non-smokers. Oral Oncol 2003;39:842-854.
23. Usta Ufuk, BerberoLu Ufuk, Helvaci Ercüment, Altaner emsi, Özdemir NecdetSÜTÇi?dem. Evaluation of cytological alterations in normal-appearing oral mucosal epithelia of smokers and nonsmokers via AgNOR counts and nuclear morphometry. Trakya Univ Tip Fak Derg 2008;25:110-116.
24. Luomanen M, Tiitta O, Heikinheimo K, Leimola-Virtanen R, Heinaro I, Happonen RP. Effect of snuff and smoking on tenascin expression in oral mucosa. J Oral Pathol Med 2006;26:334-338.
25. Applebaum KM, Furniss CS, Zeka A, et al. Lack of association of alcohol and tobacco with HPV16-associated head and neck cancer. J Natl Cancer Inst 2007;99:1801-1810.
26. Aghi MK, Liu TC, Rabkin S, Martuza RL. Hypoxia enhances the replication of oncolytic herpes simplex virus. Mol Ther 2008;10:232
27. Soysa NS, Ellepola ANB. The impact of cigarette/tobacco smoking on oral candidosis: an overview. Oral Dis 2005;11:268-273.
28. Morse DE, Katz RV, Pendrys DG, et al. Smoking and drinking in relation to oral epithelial dysplasia. Cancer Epidemiol Biomarkers Prev 1996;5:769-777.
29. Burzlaff JB, Bohrer PL, Paiva RL, et al. Exposure to alcohol or tobacco affects the pattern of maturation in oral mucosal cells: a cytohistological study. Cytopathology 2007;18:367-375.
30. Yoshioka M, St-Pierre S, Drapeau V, et al. Effects of red pepper on appetite and energy intake. Br J Nutr 1999;82:115-123.
31. Chukwu LO. Histophysiological and basal metabolic responses of albino rat, Rattus norvegicus (L), exposed to aqueous pepper extracts. African J Biotechnol 1999;5:1279-1283.
32. Final report on the safety assessment of capsicum annuum extract, capsicum annuum fruit extract, capsicum annuum resin, capsicum annuum fruit powder, capsicum frutescens fruit, capsicum frutescens fruit extract, capsicum frutescens resin, and capsaicin. Int J Toxicol 2007;26:3-106.