Multiplex PCR for detection of microcystins-producing cyanobacteria from freshwater samples

Environmental Toxicology

Volumn 25, Issue 3, 2010, Pages 251-260

  Valério, Elisabete ^a,b   Chambel, Lélia ^a   Paulino, Sérgio ^b   Faria, Natália ^b   Pereira, Paulo ^b   Tenreiro, Rogério ^a  

^a UNIVERSITY OF LISBON   (Portugal)

^b NATIONAL INSTITUTE OF HEALTH DR RICARDO JORGE   (Portugal)

Author keywords

Blooms; Cyanobacteria; Environmental samples; Microcystins; Multiplex PCR

Indexed keywords

DIRECT USE; ENVIRONMENTAL SAMPLE; FRESH WATER; GENE CLUSTERS; GENE FRAGMENTS; GOLD STANDARDS; MICROCYSTINS; MULTIPLEX PCR; PCR REACTIONS;

AMPLIFICATION; BLOOMS (METAL); GENES; LIQUID CHROMATOGRAPHY; MULTIPLEXING;

TOXIC MATERIALS;

BACTERIAL DNA; FRESH WATER; MICROCYSTIN;

ALGAL BLOOM; CYANOBACTERIUM; FRESHWATER ENVIRONMENT; IMMUNOASSAY; LIQUID CHROMATOGRAPHY; POLYMERASE CHAIN REACTION; SAMPLING; TOXICITY TEST; TOXICOLOGY;

ANABAENA; APHANIZOMENON; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; CYLINDROSPERMOPSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE AMPLIFICATION; GENE CLUSTER; GOLD STANDARD; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MICROCYSTIS AERUGINOSA; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; OSCILLATORIA; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SYNECHOCOCCUS; WATER SAMPLING;

BACTERIAL TOXINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYANOBACTERIA; DNA, BACTERIAL; ENVIRONMENTAL MONITORING; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENES, BACTERIAL; MICROCYSTINS; POLYMERASE CHAIN REACTION; WATER MICROBIOLOGY; WATER SUPPLY;

CYANOBACTERIA;

EID: 77954377976     PISSN: 15204081     EISSN: 15227278     Source Type: Journal    
DOI: 10.1002/tox.20502     Document Type: Article

References (39)

1. Azevedo SMFO, Rinehart KL, Eaglesham GK, Lau S, Carmichael WW, Jochimsen EM, Shaw GR. 2002. Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil. Toxicology 181/182:441-446. (Pubitemid 36015734)
2. Baker P. 1991. Identification of common noxious cyanobacteria. I. Nostocales. Urban Water Research Association of Australia. Research Report no 29.
3. Baker P. 1992. Identification of common noxious cyanobacteria. II. Chroococales oscillatoriales. Urban Water Research Association of Australia. Research Report no 46.
4. Baker JA, Entsch B, Neilan BA, McKay DB. 2002. Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods. Appl Environ Microbiol 68:6070-6076. (Pubitemid 36342442)
5. Batista T, de Sousa G, Suput JS, Rahmani R, Suput D. 2003. Microcystin-LR causes the collapse of actin filaments in primary human hepatocytes. Aquat Toxicol 65:85-91. (Pubitemid 37025655)
6. Bittencourt-Oliveira MC. 2003. Detection of potential microcystin-producing cyanobacteria in Brazilian reservoirs with a mcyB molecular marker. Harmful Algae 2:51-60.
7. Bourrelly P. 1970. Les Algues d'Eau Douce. Initiation à la Systématique. Tome III: Eugléniens, Péridiniens, Algues Rouges et Algues Bleus. Paris: N. Boubée & Cie.
8. Campbell MJ, Machin D. 1990. Medical Statistics: A Commonsense Approach. Chichester: Wiley.
9. Christiansen G, Fastner J, Erhard M, Börner T, Dittmann E. 2003. Microcystin biosynthesis in Planktothrix: Genes, evolution, and manipulation. J Bacteriol 185:564-572. (Pubitemid 36070483)
10. Christiansen G, Kurmayer R, Liu Q, Börner T. 2006. Transposons inactivate biosynthesis of the nonribosomal peptide microcystin in naturally occurring Planktothrix spp. Appl Environ Microbiol 72:117-123.
11. Codd GA, Lindsay J, Young FM, Morrison LF, Metcalf JS. 2005. Harmful cyanobacteria: From mass mortalities to management measures. In: Huisman J, Matthijs MHCP, Visser PM, editors. Harmful Cyanobacteria. Dordrecht, The Netherlands: Springer. pp 1-24.
12. Hisbergues M, Christiansen G, Rouhiainen L, Sivonen K, Börner T. 2003. PCR-based identification of microcystin-producing genotypes of different cyanobacterial genera. Arch Microbiol 180:420-410. (Pubitemid 38002308)
13. Kaebernick M, Dittmann E, Börner T, Neilan BA. 2002. Multiple alternate transcripts direct the biosynthesis of microcystin, a cyanobacterial nonribosomal peptide. Appl Environ Microbiol 68:449-455. (Pubitemid 34113027)
14. Komárek J, Anagnostidis K. 1986. Modern approach to the classi- fication system of Cyanophytes. II. Chroococcales. Algol Stud 43:157-226.
15. Komárek J, Anagnostidis K. 1989. Modern approach to the classification system of Cyanophytes. IV. Nostocales. Algol Stud 56:247-345.
16. Krishnamurthy T, Carmichael WW, Sarver EW. 1986. Toxic peptides from freshwater cyanobacteria (blue-green algae). I. Isolation, purification and characterization of peptides from Microcystis aeruginosa and Anabaena flos-aquae. Toxicon 24: 865-873.
17. Kurmayer R, Kutzenberger T. 2003. Application of real-time PCR for quantification of microcystin genotypes in a population of the toxic cyanobacterium Microcystis sp. Appl Environ Microbiol 69:6723-6730. (Pubitemid 37420210)
18. Kurmayer R, Christiansen G, Chorus I. 2003. The abundance of microcystin-producing genotypes correlates positively with colony size in Microcystis sp. and determines its microcystin net production in Lake Wannsee. Appl Environ Microbiol 69: 787-795.
19. Kurmayer R, Christiansen G, Fastner J, Börner T. 2004. Abundance of active and inactive microcystin genotypes in populations of the toxic cyanobacterium Planktothrix spp. Environ Microb 6:831-841.
20. Kurmayer R, Christiansen G, Gumpenberger M, Fastner J. 2005. Genetic identification of microcystin ecotypes in toxic cyanobacteria of the genus Planktothrix. Microbiology 151:1525-1533.
21. Matsunaga H, Harada K-I, Senma M, Ito Y, Yasuda N, Ushida S, Kimura Y. 1999. Possible cause of unnatural mass death of wild birds in a pond in Nishinomiya, Japan: Sudden appearance of toxic cyanobacteria. Nat Toxins 7:81-84.
22. Mbedi S, Welker M, Fastner J, Wiedner C. 2005. Variability of the microcystin synthetase gene cluster in the genus Planktothrix (Oscillatoriales: Cyanobacteria). FEMS Microbiol Lett 245:299-306.
23. Mikalsen B, Boison G, Skulberg OM, Fastner J, Davies W, Gabrielsen TM, Rudi K, Jakobsen KS. 2003. Natural variation in the microcystin synthetase operon mcyABC and impact on microcystin production in Microcystis strains. J Bacteriol 185:2774-2785. (Pubitemid 36514588)
24. Neilan BA, Dittmann E, Rouhiainen L, Bass RA, Schaub V, Sivonen K, Börner T. 1999. Nonribosomal peptide synthesis and toxigenicity of cyanobacteria. J Bacteriol 181:4089-4097. (Pubitemid 29295929)
25. Nübel U, Garcial-Pichel F, Muyzer G. 1997. PCR primers to amplify 16S rRNA genes from Cyanobacteria. Appl Environ Microbiol 63:3327-3332. (Pubitemid 27337840)
26. Ouahid Y, Pérez-Silva G, del Campo FF. 2005. Identification of potentially toxic environmental microcystis by individual and multiple PCR amplification of specific microcystin synthetase gene regions. Environ Toxicol 20:235-242. (Pubitemid 40827440)
27. Pan H, Song L, Liu Y, Börner T. 2002. Detection of hepatotoxic Microcystis strains by PCR with intact cells from both culture and environment samples. Arch Microbiol 178:421-427. (Pubitemid 35340972)
28. Preisendorfer RW. 1986. Secchi disk science: Visual optics of natural waters. Limnol Oceanogr 31:909-926.
29. Rantala A, Fewer DP, Hisbergues M, Rouhiainen L, Vaitomaa J, Börner T, Sivonen K. 2004. Phylogenetic evidence for the early evolution of microcystin synthesis. Proc Natl Acad Sci USA 101:568-573.
30. Rouhiainen L, Vakkilainen T, Siemer BL, Buikema W, Haselkorn R, Sivonen K. 2004. Genes coding for hepatotoxic heptapeptides (microcystins) in the cyanobacterium Anabaena strain 90. Appl Environ Microbiol 70:686-692. (Pubitemid 38568189)
31. Sivonen K, Jones GJ. 1999. Cyanobacterial toxins. In: Chorus I, Bartram J, editors. Toxic Cyanobacteria in Water. A Guide to their Public Health Consequences, Monitoring and Management. London: WHO. E & FN Spon. pp 41-111.
32. Skulberg R, Skulberg OM. 1990. Forskning Med Algekulturer NIVAs Kultursampling av Alger. Research with Algal Cultures. NIVA's Culture Collection of Algae. Oslo, Norway: Norsk Institutt for Vannforskning.
33. Tillett D, Dittmann E, Erhard M, von Döhren H, Börner T, Neilan BA. 2000. Structural organization of microcystin biosynthesis in Microcystis aeruginosa PCC 7806: An integrated peptidepolyketide synthetase system. Chem Biol 7:753-764.
34. Tillett D, Parker DL, Neilan BA. 2001. Detection of toxigenicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis: Comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Appl Environ Microbiol 67:2810-2818. (Pubitemid 33639761)
35. Vaitomaa J, Rantala A, Halinen K, Rouhiainen L, Tallberg P, Mokelke L, Sivonen K. 2003. Quantitative real-time PCR for determination of microcystin synthetase E copy numbers for Microcystis and Anabaena in Lakes. Appl Environ Microbiol 69:7289-7297.
36. Valério E, Pereira P, Saker ML, Franca S, Tenreiro R. 2005. Molecular characterization of Cylindrospermopsis raciborskii strains isolated from Portuguese freshwaters. Harmful Algae 4:1044-1052.
37. Valério E, Chambel L, Paulino S, Faria N, Pereira P, Tenreiro R. 2009. Molecular identification, typing and traceability of cyanobacteria from freshwater reservoirs. Microbiology 155:642-656.
38. WHO. 1998. Cyanobacterial Toxins: Microcystin-LR. Guideline for Drinking-Water Quality. Addendum to Volume 2. Geneva: World Health Organization. pp 95-110.
39. Yu S, Zhao N, Zi X. 2001. The relationship between cyanotoxin (microcystin. MC) in pond-ditch water and primary liver cancer in China. Zhonghua Zhong Liu Za Zhi 23:96-99.