Site-specific fluorescent probing of RNA molecules by unnatural base-pair transcription for local structural conformation analysis

Nature Protocols

Volumn 5, Issue 7, 2010, Pages 1312-1323

  Hikida, Yasushi ^a,b,c   Kimoto, Michiko ^a,b   Yokoyama, Shigeyuki ^b,c   Hirao, Ichiro ^a,b  

^a TagCyx Biotechnologies   (Japan)

^b RIKEN   (Japan)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINO 6 (2 THIENYL)PURINE; 2 PYRROLECARBOXALDEHYDE; FLUORESCENT DYE; NUCLEOSIDE TRIPHOSPHATE; TRANSFER RNA; UNCLASSIFIED DRUG; 2-AMINO-6-(2-THIENYL)PURINE; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; PURINE DERIVATIVE; PYRROLE DERIVATIVE; PYRROLE-2-CARBOXALDEHYDE; RNA; VIRUS PROTEIN;

ANALYTIC METHOD; ARTICLE; BASE PAIRING; CONFORMATIONAL TRANSITION; DNA TEMPLATE; FLUORESCENCE ANALYSIS; GENETIC ANALYSIS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; RNA PROBE; RNA PURIFICATION; RNA TRANSCRIPTION; STRUCTURE ANALYSIS; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; ULTRASTRUCTURE;

BASE PAIRING; DNA-DIRECTED RNA POLYMERASES; FLUORESCENT DYES; PURINES; PYRROLES; RNA; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 77954306363     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.77     Document Type: Article

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