Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples;Detecção de mórulas e DNA de Ehrlichia canis em sangue e em aspirado de baço em cães naturalmente infectados

Revista Brasileira de Parasitologia Veterinaria

Volumn 19, Issue 2, 2010, Pages 24-28

  Faria, Joice Lara Maia ^a,c   Dagnone, Ana Sílvia ^b   Munhoz, Thiago Demarchi ^c   João, Carolina Franchi ^c   Pereira, Wanderson Adriano Biscola ^a   Machado, Rosângela Zacarias ^c   Tinucci Costa, Mirela ^c  

^a UNIVERSITY OF UBERABA   (Brazil)

^b Hospital Universitário   (Brazil)

^c SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Dogs; Ehrlichia canis; Morulae; nPCR; Spleen

Indexed keywords

CANIS FAMILIARIS; EHRLICHIA CANIS;

EID: 77954288539     PISSN: 0103846X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. BOWDLER, A. J. The Clinical significance of the spleen. In: BOWDLER, A. J. The Complete Spleen: structure, function, and clinical disorders. Second Edition, New Jersey: Humana Press Inc, 2002. p 139-156.
2. CADMAN, H. F. et al. Comparison of the dot-blot enzyme linked immunoassay with immunofluorescence for detecting antibodies to Ehrlichia canis. Veterinary Record, v. 135, n. 15, p. 362, 2004.
3. CASTRO, M. B. et al. Experimental acute canine monocytic ehrlichiosis: clinicopathologic and immunopathological findings. Veterinary Parasitology, v. 119, n. 1, p. 73-86, 2004.
4. CODNER, E. C.; FARRIS-SMITH, L. L. Characterization of the subclinical phase of ehrlichiosis in dogs. Journal American Veterinary Medical Association, v. 189, n. 1, p. 47-50, 1986.
5. DAGNONE, A. S.; MORAIS, H. S. A.; VIDOTTO, O. Erliquiose nos animais e no homem. Semina: Ciências Agrárias, v. 22, n. 2, p. 115-248, 2001.
6. DAVIDSON, R. N.; WALL, R. A. Prevention and management of infections in patients without a spleen. Clinical Microbiology Infection, v. 7, n. 12, p. 657-660, 2001.
7. ELIAS, E. Diagnosis of ehrlichiosis from the presence of inclusion bodies or morulae of E. canis. Journal of Small Animal Practice, v. 33, n. 11, p. 540-543, 1991.
8. FOLEY, J. E. et al. A simian model of human granulocytic ehrlichiosis. American Journal of Tropical Medicine and Hygiene, v. 60, n. 6, p. 987-993, 1999.
9. FURUTA, P. I. et al. Comparison between a soluble antigen-based ELISA and IFAT in detecting antibodies against Babesia canis in dogs. Revista Brasileira de Parasitologia Veterinária, v. 18, n. 3, p. 41-45, 2009.
10. FRANK, J. R.; BREITSCHWERDT, E. B. A retrospective study of ehrlichiosis in 62 dogs from North Carolina and Virginia. Journal of Veterinary Internal Medicine, v. 13, n. 3, p. 194-201, 1999.
11. HARRUS, S.; BARK, H.; WANER, T. Canine monocytic ehrlichiosis: an update. Compendium on Continuing Education for the Practicing Veterinarian, v. 19, n. 4, p. 431-447, 1997.
12. HARRUS, S. et al. Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. Journal of Clinical Microbiology, v. 36, n. 1, p. 73-76, 1998a.
13. HARRUS, S. et al. Investigation of splenic functions in canine monocytic ehrlichiosis. Veterinary Immunology Immunopathology, v. 62, n. 1, p. 15-27, 1998b.
14. HARRUS, S. et al. Recent advances in determining the pathogenesis of canine monocytic ehrlichiosis. Journal of Clinical Microbiology, v. 37, n. 9, p. 2745-2749, 1999.
15. HARRUS, S. et al. Comparison of simultaneous splenic sample PCR with blood sample PCR for diagnosis and treatment of experimental Ehrlichia canis infection. Antimicrobial Agents and Chemotherapy, v. 48, n. 11, p. 4488-4490, 2004.
16. LEWIS, G. E. et al. The brown dog tick Rhipicephalus sanguineus and the dog as experimental hosts of Ehrlichia canis. American Journal Veterinary Research, v. 38, n. 12, p. 1953-1955, 1977.
17. MYLONAKIS, M. E. et al. Evaluation of cytology in the diagnosis of acute canine monocytic ehrlichiosis (Ehrlichia canis): a comparison between five methods. Veterinary Microbiology, v. 91, n. 2-3, p. 197-204, 2003.
18. MURPHY, G. L. et al. A molecular and serologic survey of Ehrlichia canis, E. chaffeensis, and E. ewingii in dogs and ticks from Oklahoma. Veterinary Parasitology, v. 79, n. 4, p. 325-329, 1998.
19. NAKAGHI, A. C. H. et al. Canine ehrlichiosis: clinical, hematological, serological and molecular aspects. Ciência Rural, v. 38, n. 3, p. 766-770, 2008.
20. NEER, M. T.; HARRUS, S. Ehrlichiosis, Neorickettsiosis, Anaplasmosis and Wolbachia infection. In: GREENE, C. E. Infectious diseases of the dog and cat. Philadelphia: Elsevier, 2006. p. 203-216.
21. OLIVEIRA, D. et al. Anti-Ehrlichia canis antibodies detection by "Dot-ELISA" in naturally infected dogs. Revista Brasileira de Parasitologia Veterinária, v. 9, n. 1, p. 1-5, 2000.
22. RABINSTEIN, A. et al. Recurrent and prolonged fever in asplenic patients with human granulocytic ehrlichiosis. QJ Medicine, v. 93, n. 3, p. 198-201, 2000.
23. WANER, T. et al. Demonstration of serum antiplatelet antibodies in experimental acute canine ehrlichiosis. Veterinary Immunology Immmunopathology, v. 48, n. 1-2, p. 177-182, 1995.
24. WANER, T. et al. Characterization of subclinical phase of canine ehrlichiosis in experimentally infected beagles dogs. Veterinary Parasitology, v. 69, n. 3-4, p. 307-317, 1997.
25. WANER, T. et al. Canine Monocytic Ehrlichiosis - an overview. Israel Journal of Veterinary Medicine, v. 54, n. 4, p. 103-107, 1999.
26. WEN, B. et al. Comparison of nested PCR with Immunofluorescent - antibody Assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. Journal of Clinical Microbiology, v. 35, n. 7, p. 1852-1855, 1997.