Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis;Avaliação da sensibilidade da PCR em uma etapa com base no gene p28 de Ehrlichia canis e sua aplicação no diagnóstico da erliquiose canina

Revista Brasileira de Parasitologia Veterinaria

Volumn 19, Issue 2, 2010, Pages 1-5

  Nakaghi, Andrea Cristina Higa ^a   Machado, Rosangela Zacarias ^a   Ferro, Jesus Aparecido ^a   Labruna, Marcelo Bahia ^b   Chryssafidis, Andreas Lazaros ^b   André, Marcos Rogério ^a   Baldani, Cristiane Divan ^c  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSIDADE FEDERAL DO TOCANTINS   (Brazil)

Author keywords

16s rRNA gene; dsb gene; Ehrlichia canis; p28; PCR

Indexed keywords

CANIS FAMILIARIS; EHRLICHIA; EHRLICHIA CANIS;

EID: 77954287956     PISSN: 0103846X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. AGUIAR, D. M. et al. Prevalence of Ehrlichia canis (Rickettsiales: Anaplasmataceae) in dogs and Rhipicephalus sanguineus (Acari: Ixodidae) ticks from Brazil. Journal of medical entomology, v. 44, n. 1, p. 126-132, 2007.
2. BREITSCHWERDT, E. B.; HEGARTY, B. C.; HANCOCK, S. I. Doxycycline hyclate treatment of experimental canine ehrlichiosis followed by challenge inoculation with two Ehrlichia canis strains. Antimicrobial Agents Chemother, v. 42, n. 2, p. 362-368, 1998.
3. BULLA, C. et al. The relationship between the degree of thrombocytopenia and infection with Ehrlichia canis in an endemic area. Veterinary Research, v.35, n. 1, p. 141-146, 2004.
4. CASTRO, M. B. et al. Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings. Veterinary Parasitology, v. 119, n. 1, p. 73-86, 2004.
5. CHANG, W. L; PAN, M. J. Specific amplification of Ehrlichia platys dna from blood specimens by two-step PCR. Journal Clinical Microbiology, v. 34, n. 12, p. 3142-3146, 1996.
6. COHN, L. A. Ehrlichiosis and related infections. Veterinary Clinics Small Animals, v. 33, n. 4, p. 863-884, 2003.
7. COSTA, J. O. et al. Ehrlichia canis infection in dog in Belo Horizonte: Brazil. Arquivos da Escola de Veterinária da Universidade Federal de Minas Gerais, v. 25, n. 2, p. 199-200, 1973.
8. DAGNONE, A. S. et al. Ehrlichiosis in anemic, thrombocytopenic, or tick-infested dogs from a hospital population in South Brazil. Veterinary Parasitology, v. 117, n. 4, p. 285-290, 2003.
9. DUMLER, J. S. Molecular methods for ehrlichiosis and Lyme disease. Clinics in Laboratoty Medicine, v. 23, n. 4, p. 867-884, 2003.
10. ENGVALL, E. O. et al. A 16S rRNA-based PCR assay for detection and identification of granulocytic Ehrlichia species in dogs, horses, and cattle. Journal Clinical Microbiology, v. 34, n. 9, p. 2170-2174, 1996.
11. HARRUS, S. et al. Amplification of ehrlichial DNA from dogs 34 months after infection with Ehrlichia canis. Journal Clinical Microbiology, v. 36, n. 1, p. 73-76, 1998.
12. IQBAL, Z.; CHAICHANASIRIWITHAYA, W.; RIKIHISA, Y. Comparison of PCR with other tests for early diagnosis of canine ehrlichiosis. Journal Clinical Microbiology, v. 32, n. 7, p. 1658-1662, 1994.
13. IQBAL, Z.; RIKIHISA, Y. Application of the polymerase chain reaction for the detection of Ehrlichia canis in tissues of dogs. Veterinary Microbiology, v. 42, n. 4, p. 281-287, 1994.
14. KWOK, S.; HIGUCHI, R. Avoiding false positives with PCR. Nature, v. 339, n. 6221 p. 237-238, 1989.
15. LABRUNA, M. B. et al. A preliminary investigation of Ehrlichia species in ticks, humans, dogs, and capybaras from Brazil. Veterinary Parasitology, v. 143, n. 2, p. 189-195, 2007.
16. MACHADO, R. Z. Erliquiose canina. Revista Brasileira de Parasitologia Veterinária, v. 13, supl. 1, p. 53-57, 2004.
17. MACIEIRA, D. B. et al. Prevalence of Ehrlichia canis infection in thrombocytopenic dogs from Rio de Janeiro, Brazil. Veterinary Clinical Pathology, v. 34, n. 1, p. 44-48, 2005.
18. MCBRIDE, J. W.; YU, X. J.; WALKER, D. H. A conserved, transcriptionally active p28 multigene locus of Ehrlichia canis. Gene, v. 254, n. 1-2, p. 254-252, 2000.
19. MCBRIDE, J. W.; YU, X. J.; WALKER, D. H. Molecular cloning of the gene for a conserved major immunoreactive 28 kilodalton of Ehrlichia canis: a potential serodiagnostic antigen. Clinical and Diagnostic Laboratory Immunology, v. 6, n. 3, p. 392-399, 1999.
20. MURPHY, G. L. et al. A molecular and serologic survey of Ehrlichia canis, E. chaffeensis and E. ewingii in dogs and ticks from Oklahoma. Veterinary Parasitology, v. 79, n. 4, p. 325-339, 1998.
21. NAKAGHI, A. C. H. et al. Canine ehrlichiosis: clinical, hematological, serological and molecular aspects. Ciência Rural, v. 38, n. 3, p. 766-770, 2008.
22. SANTOS, F. et al. Molecular evaluation of the incidence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil. Veterinary Journal, v. 179, n. 1, p. 145-148, 2009.
23. SEAMAN, R. L. et al. Comparison of results for serologic testing a polymerase chain reaction assay to determine the prevalence of stray dogs in eastern Tennessee seropositive to Ehrlichia canis. American Journal Veterinary Research, v. 65, n. 9, p. 1200-1203, 2004.
24. STICH, R. W. et al. Detection of Ehrlichia canis in canine carrier blood and in individual experimentally infected ticks with a p30-based PCR assay. Journal Clinical Microbiology, v. 40, n. 2, p. 540-546, 2002.
25. SUMNER, J. W.; NICHOLSON, W. L.; MASSUNG, R. F. PCR amplification and comparison of nucleotide sequences from groESL shock operon of Ehrlichia species. Journal Clinical Microbiology, v. 35, n. 8, p. 2087-2092, 1997.
26. WAGNER, E. T. Development of a p28-based PCR assay for Ehrlichia chaffeensis. Molecular and Cellular Probes, v. 18, n. 2, p. 111-116, 2004.
27. WEN, B. et al. Comparison of nested PCR with immunofluorescent-antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline. Journal Clinical Microbiology, v. 35, n. 7, p. 1852-1855, 1997.
28. WHITLOCK, J. E. Prevalence of Ehrlichia chaffeensis (Rickettsiales: Rickettsiaceae) in Amblyomma americanum (Acari: Ixodidae) from the Georgia coast and barrier Islands. Journal Medicine Entomology, v. 37, n. 2, p. 276-280, 2000.