Dilution of boar ejaculates with BTS containing HEPES in place of bicarbonate immediately after ejaculation can reduce the increased inducibility of the acrosome reaction by treatment with calcium and calcium ionophore A23187, which is potentially associated with boar subfertility

Journal of Reproduction and Development

Volumn 56, Issue 3, 2010, Pages 309-314

  Murase, Tetsuma ^a   Imaeda, Noriaki ^a,b   Yamada, Hiroto ^b   Takasu, Masaki ^a   Taguchi, Kazuo ^b   Katoh, Tsutomu ^b  

^a GIFU UNIVERSITY   (Japan)

^b Gifu Prefectural Livestock Research Institute   (Japan)

Author keywords

Acrosome reaction; Bicarbonate; Boar; Spermatozoa; Storage

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; BICARBONATE; CALCIMYCIN; CALCIUM; CULTURE MEDIUM;

ACROSOME REACTION; ANIMAL; ARTICLE; CELL SURVIVAL; CHEMISTRY; CULTURE MEDIUM; DRUG EFFECT; EJACULATION; INSTRUMENTATION; MALE; METABOLISM; METHODOLOGY; SPERM; SPERM PRESERVATION; SPERMATOZOON; SPERMATOZOON MOTILITY; SWINE; DEVICES; DRUG EFFECTS; PROCEDURES;

ACROSOME REACTION; ANIMALS; BICARBONATES; CALCIMYCIN; CALCIUM; CELL SURVIVAL; CULTURE MEDIA; EJACULATION; HEPES; MALE; SEMEN; SEMEN PRESERVATION; SPERM MOTILITY; SPERMATOZOA; SUS SCROFA;

SUIDAE;

EID: 77954270810     PISSN: 09168818     EISSN: 13484400     Source Type: Journal    
DOI: 10.1262/jrd.09-157N     Document Type: Article

References (27)

1. Love RJ. Seasonal infertility in pigs. Vet Rec 1981; 109: 407-409.
2. Stork MG. Seasonal reproductive inefficiency in large pig breeding units in Britain. Vet Rec 1979; 104: 49-52.
3. Murase T, Imaeda N, Yamada H, Miyazawa K. Seasonal changes in semen characteristics, composition of seminal plasma and frequency of acrosome reaction induced by calcium and calcium ionophore A23187 in large white boars. J Reprod Dev 2007; 53: 853-865.
4. Harrison RA, Gadella BM. Bicarbonate-induced membrane processing in sperm capacitation. Theriogenology 2005; 63: 342-351.
5. Ashworth PJ, Harrison RA, Miller NG, Plummer JM, Watson PF. Flow cytometric detection of bicarbonate-induced changes in lectin binding in boar and ram sperm populations. Mol Reprod Dev 1995; 40: 164-176.
6. Harrison RA, Mairet B, Miller NG. Flow cytometric studies of bicarbonate-mediated Ca2+ influx in boar sperm populations. Mol Reprod Dev 1993; 35: 197-208.
7. Maxwell WMC, Johnson LA. Physiology of spermatozoa at high dilution rates: the influence of seminal plasma. Theriogenology 1999; 52: 1353-1362.
8. Holt C, Holt WV, Moore HD, Reed HC, Curnock RM. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J Androl 1997; 18: 312-323.
9. Januskauskas A, Johannisson A, Soderquist L, Rodriguez-Martinez H. Assessment of sperm characteristics post-thaw and response to calcium ionophore in relation to fertility in Swedish dairy AI bulls. Theriogenology 2000; 53: 859-875.
10. Johnson LA, Weitze KF, Fiser P, Maxwell WMC. Storage of boar semen. Anim Reprod Sci 2000; 62: 143-172.
11. Johnson LA, Aalbers JG, Grooten HJG. Artificial insemination of swine: fecundity of boar semen stored in Beltsville TS (BTS), modified Modena (MM), or MR-A and inseminated on one, three and four days after collection. Zuchthyg 1988; 23: 49-55.
12. Roldan ERS, Murase T. Polyphosphoinositide-derived diacylglycerol stimulates the hydrolysis of phosphatidylcholine by phospholipase C during exocytosis of the ram sperm acrosome. Effect is not mediated by protein kinase C. J Biol Chem 1994; 269: 23583-23589.
13. Murase T, Imaeda N, Kondoh N, Tsubota T. Ceramide enhances acrosomal exocytosis triggered by calcium and the calcium ionophore A23187 in boar spermatozoa. J Reprod Dev 2004; 50: 667-674.
14. Shams-Borhan G, Harrison RAP. Production, characterization, and use of ionophore-induced, calcium-dependent acrosome reaction in ram spermatozoa. Gamete Res 1981; 4: 407-432.
15. Harrison RA, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil 1990; 88: 343-352.
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275.
17. Rodriguez-Martinez H, Ekstedt E, Einarsson S. Acidification of epididymal fluid in the boar. Int J Androl 1990; 13: 238-243.
18. Boursnell JC, Baronos S, Briggs PA, Butler EJ. The concentrations of zinc in boar seminal plasma and vesicular secretion in relation to those of nitrogenous substances, citrate, galactose and fructose. J Reprod Fertil 1972; 29: 215-227.
19. Arver S, Eliasson R. Zinc and magnesium in bull and boar spermatozoa. J Reprod Fertil 1980; 60: 481-484.
20. Okamura N, Tajima Y, Soejima A, Masuda H, Sugita Y. Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through direct activation of adenylate cyclase. J Biol Chem 1985; 260: 9699-9705.
21. Witte TS, Schafer-Somi S. Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Anim Reprod Sci 2007; 102: 181-193.
22. Dostalova Z, Calvete JJ, Sanz L, Töpfer-Petersen E. Quantitation of boar spermadhesins in accessory sex gland fluids and on the surface of epididymal, ejaculated and capacitated spermatozoa. Biochim Biophys Acta 1994; 1200: 48-54.
23. Garcia EM, Vazquez JM, Calvete JJ, Sanz L, Caballero I, Parrilla I, Gil MA, Roca J, Martinez EA. Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality. J Androl 2006; 27: 434-443.
24. Johnson LA, Weitze KF, Fiser P, Maxwell WM. Storage of boar semen. Anim Reprod Sci 2000; 62: 143-172.
25. Harrison RAP. Sperm plasma membrane characteristics and boar semen fertility. J Reprod Fertil Suppl 1997; 52: 195-211.
26. Garde J, Roldan ER. Stimulation of Ca2+-dependent exocytosis of the sperm acrosome by cAMP acting downstream of phospholipase A2. J Reprod Fertil 2000; 118: 57-68.
27. Garde J, Roldan ER. rab 3-peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A2 metabolites. FEBS Lett 1996; 391: 263-268.