In vitro and in vivo hepatoprotective effects of the total alkaloid fraction of Hygrophila auriculata leaves

Indian Journal of Pharmacology

Volumn 42, Issue 2, 2010, Pages 99-104

  Raj, Vasanth P ^a   Chandrasekhar, Raghu H ^a   Vijayan, P ^b   Dhanaraj, S A ^b   Rao, Mallikarjuna C ^a   Rao, Venkata J ^a   Nitesh, K ^a  

^a MANIPAL COLLEGE OF PHARMACEUTICAL SCIENCES   (India)

^b MANIPAL UNIVERSITY   (India)

Author keywords

hepatocytes; hepatoprotective; HepG2; Hygrophila auriculata; total alkaloidsIntroduction

Indexed keywords

ALKALOID; CARBON TETRACHLORIDE; HYGROPHILA AURICULATA EXTRACT; LIVER PROTECTIVE AGENT; METHANOL; PLANT EXTRACT; SILYMARIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; DOSE RESPONSE; DRUG EFFECT; DRUG EXPOSURE; DRUG ISOLATION; FEMALE; FRACTIONATION; HISTOPATHOLOGY; HYGROPHILA AURICULATA; IN VITRO STUDY; IN VIVO STUDY; LIVER CELL; LIVER INJURY; LIVER PROTECTION; LIVER TOXICITY; MALE; MEDICINAL PLANT; NONHUMAN; PLANT LEAF; RAT; THIN LAYER CHROMATOGRAPHY; WISTAR ALBINO GLAXO RAT;

EID: 77954222301     PISSN: 02537613     EISSN: 19983751     Source Type: Journal    
DOI: 10.4103/0253-7613.64500     Document Type: Article

References (27)

1. Warrier PK, Nambiar VP, Ramankutty C, Vaidyarathnam P.S. Varrier's Indian Medicinal Plants, A Compendium of 500 Species.Vol. 1. India: Orient Longman; 1995. p. 191-196
2. Vijayakumar M, Govindarajan R, Rao GM, Rao CV, Shirwaikar A, Mehrotra S, et al. Action of Hygrophila auriculata against streptozotocin-induced oxidative stress. J Ethnopharmacol 2006;104:356-361
3. Prajapat ND, Kumar U. Medicinal Plants: An overview. Agro's dictionary of medical plants. 1st ed. India: Agrobios; 2005. p. 164-165
4. Heftmann E. Biochemistry of plant steroids. Annu Rev Plant Physiol 1963;14:225-248.
5. Trease GW, Evans WC. Alkaloids. Pharmacognosy. 15th ed. India: Elsevier Saunders; 2005. p. 333-338
6. Seglen PO. Hepatocyte suspensions and cultures as tools in experimental carcinogenesis. J Toxicol Environ Health 1979;5:551-560
7. Freshney I. Culture of animal cells: A manual of basic technique. Quantitation. 4th ed. New York. Wiley-Liss; 2000. p. 331-332
8. Kiso Y, Tohkin M, Hikino H. Assay method for antihepatotoxic activity using carbon tetrachloride induced cytotoxicity in primary cultured hepatocytes Planta Med 1983;49:222-225 (Pubitemid 14220883)
9. Thabrew MI, Hughes RD, McFarlane IG. Screening of hepatoprotective plant components using a HepG2 cell cytotoxicity assay. J Pharm Pharmacol 1997;49:1132-1135
10. Hu K, Kobayashi H, Dong A, Jing Y, Iwasaki S, Yao X. Antineoplastic agents, III: Steroidal glycosides from Solanum nigrum. Planta Med 1999;65:35-38
11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-275
12. Lin SC, Lin CH, Lin CC, Lin YH, Chen CF, Chen IC, et al. Hepatoprotective effects of Arctium lappa Linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. J Biomed Sci 2002;9:401-409
13. Clawson GA. Mechanisms of carbon tetrachloride hepatotoxicity. Pathol Immunopathol Res 1989;8:104-112
14. Das K, Chowdhury A. Hepatic fibrosis: Can we treat it clinically? Trop Gastroenterol 2008;29:76-83.
15. Rubinstein D. Epinephrine release and liver glycogen levels after carbon tetrachloride administration. Am J Physiol 1962;203:1033-1037
16. Manjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A, et al. Effect of Phyllanthus niruri Linn. treatment on liver, kidney and testes in CCl4 induced hepatotoxic rats. Indian J Exp Biol 2008;46:514-520
17. Lee HS, Jung KH, Hong SW, Park IS, Lee C, Han HK, et al. Morin protects acute liver damage by carbon tetrachloride (CCl(4)) in rat. Arch Pharm Res 2008;31:1160-1165
18. Bishayee A, Sarkar A, Chatterjee M. Hepatoprotective activity of carrot (Daucus carota L.) against carbon tetrachloride intoxication in mouse liver. J Ethnopharmacol 1995;47:69-74.
19. Castro JA, De Ferreyra EC, De Castro CR, De Fenos OM, Sasame H, Gillette JR. Prevention of carbon tetrachloride-induced necrosis by inhibitors of drug metabolism-further studies on their mechanism of action. Biochem Pharmacol 1974;23:295-302.
20. Ramellini G, Meldolesi J. Liver protection by silymarin: In vitro effect on dissociated rat hepatocytes. Arzneimittelforschung 1976;26:69-73.
21. Gomes A, Das M, Dasgupta SC. Haematinic effect of Hygrophila spinosa T: Anderson on experimental rodents. Indian J Exp Biol 2001;39:381-382
22. Mazumdar UK, Gupta M, Maiti S. Effect of petroleum ether extract from Hygrophila spinosa on hematological parameters and hepatorenal functions in mice. Indian J Exp Biol 1996;34:1201-1203
23. Mazumdar UK, Gupta M, Maiti S, Mukherjee D. Antitumor activity of Hygrophila spinosa on Ehrlich ascites carcinoma and sarcoma-180 induced mice. Indian J Exp Biol 1997;35:473-477
24. Dewanji A, Chanda S, Si L, Barik S, Matai S. Extractability and nutritional value of leaf protein from tropical aquatic plants. Plant Foods Hum Nutr 1997;50:349-357
25. Usha K, Mary Kasturi G, Hemalatha P. Hepatoprotective effect of Hygrophila spinosa and Cassia occidentalis on carbon tetrachloride induced liver damage in experimental rats. Indian J Clin Biochem 2007;22:132-135
26. Singh A, Handa SS. Hepatoprotective activity of Apium graveolens and Hygrophila auriculata against paracetamol and thioacetamide intoxication in rats. J Ethnopharmacol 1995;49:119-126
27. Shanmugasundaram P, Venkataraman S. Hepatoprotective and antioxidant effects of Hygrophila auriculata (K. Schum) Heine Acanthaceae root extract. J Ethnopharmacol 2006;104:124-128