Mass spectrometry-based thermal shift assay for protein-ligand binding analysis

Analytical Chemistry

Volumn 82, Issue 13, 2010, Pages 5573-5581

  West, Graham M ^a   Thompson, J Will ^a   Soderblom, Erik J ^a   Dubois, Laura G ^a   Dearmond, Patrick D ^a   Moseley, M Arthur ^a   Fitzgerald, Michael C ^a  

^a DUKE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING INTERACTION; BOVINE CARBONIC ANHYDRASE; CYCLOPHILIN A; CYCLOSPORIN A; INTACT PROTEINS; LIGAND BINDING; MASS SPECTRAL DATA; METHIONINE RESIDUES; MULTICOMPONENTS; NEW PROTOCOL; OXIDATION REACTIONS; PROOF OF PRINCIPLES; PROTEIN MIXTURES; PROTEIN MODELS; REACTION TIME; RIBONUCLEASE A; SCREENING ASSAYS; SOLVENT ACCESSIBILITY; THERMAL DENATURATIONS; THERMAL SHIFT; TRYPTIC PEPTIDES; UBIQUITIN;

BINDING ENERGY; CARBONIC ANHYDRASE; HYDROGEN PEROXIDE; LIGANDS; MASS SPECTROMETRY; OXIDATION; PYROLYSIS;

PROTEINS;

CARBONATE DEHYDRATASE II; CYCLOPHILIN A; HYDROGEN PEROXIDE; LIGAND; METHIONINE; PANCREATIC RIBONUCLEASE; PROTEIN; TRYPSIN; UBIQUITIN; PROTEIN BINDING;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PROTEIN BINDING; TEMPERATURE; BOVINAE; PROCEDURES;

AMINO ACID SEQUENCE; ANIMALS; CARBONIC ANHYDRASE II; CATTLE; CHROMATOGRAPHY, LIQUID; CYCLOPHILIN A; HYDROGEN PEROXIDE; LIGANDS; METHIONINE; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PROTEIN BINDING; PROTEINS; RIBONUCLEASE, PANCREATIC; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; TRYPSIN; UBIQUITIN;

EID: 77954190803     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac100465a     Document Type: Article

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