Visualization of skeletons and intervertebral disks in live fish larvae by fluorescent calcein staining and disk specific GFP expression

Journal of Applied Ichthyology

Volumn 26, Issue 2, 2010, Pages 268-273

  Haga, Y ^a   Du, S J ^b   Masui, S ^a   Fujinami, Y ^c   Aritaki, M ^c   Satoh, S ^a  

^a TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

^b UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^c JAPAN FISHERIES RESEARCH AND EDUCATION AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMALIA; ARCHOSARGUS RHOMBOIDALIS; DANIO RERIO; ORYZIAS; ORYZIINAE;

EID: 77954181435     PISSN: 01758659     EISSN: 14390426     Source Type: Journal    
DOI: 10.1111/j.1439-0426.2010.01418.x     Document Type: Article

References (29)

1. Cahu, C.; Zambonino Infante, J.; Takeuchi, T., 2003: Nutritional components affecting skeletal development in fish larvae. Aquaculture 227, 245-258.
2. Choi, K.-S.; Cohn, M. J.; Harfe, B. D., 2008: Identification of nucleus pulposus precursorcells and notochordal remnants in the mouse: implications for disk degeneration and chordma formation. Dev. Dyn. 237, 3953-3958.
3. Du, S. J.; Dienhart, M., 2001: Zebrafish tiggy winkle hedgehog promoter directs notochord and floor plate green fluorescent protein expression in transgenic zebrafish embryos. Dev. Dyn. 222, 655-666.
4. Du, S. J.; Haga, Y., 2004: The zebrafish as a model for studying skeletal development. In: Biomineralization: progress in biology, molecular biology and application, 2nd edn. E. Baeuerlein (Ed.). Wiley-VCH, Weinheim, pp. 283-304.
5. Du, S. J.; Devoto, S. H.; Westerfield, M.; Moon, R. T., 1997: Positive and negative regulation of muscle cell identity by members of the Hedgehog and TGF-β gene families. J. Cell Biol. 139, 145-156.
6. Du, S. J.; Frenkel, V.; Kindschi, G.; Zohar, Y., 2001: Visualizing normal and defective bone development in zebrafish embryos using the fluorescent chromophore calcein. Dev. Biol. 238, 239-246.
7. Ertzer, R.; Muller, F.; Hadzhiev, Y.; Rathnam, S.; Fischer, N.; Rastegar, S.; Strahle, U., 2007: Cooperation of sonic hedgehog enhancers in midline expression. Dev. Biol. 3011, 578-589.
8. Gavaia, P. J.; Sarasquete, C.; Cancela, M. L., 2000: Detection of mineralized structures in early stages of development of marine teleostei using a modified alcian blue-alizarin red double staining technique for bone and cartilage. Biotech. Histochem. 75, 79-84.
9. Grotmol, S.; Nordvik, K.; Kryvi, H.; Totland, G. K., 2005: A segmental pattern of alkaline phosphatase activity within the notochord coincides with the initial formation of the vertebral bodies. J. Anat. 206, 427-436.
10. Haga, Y.; Takeuchi, T.; Seikai, T., 2001: Influence of all-trans retinoic acid on pigmentation and skeletal formation in larval Japanese flounder. Fish. Sci. 68, 560-570.
11. Haga, Y.; Suzuki, T.; Kagechika, H.; Takeuchi, T., 2003: A retinoic acid receptor-selective agonist causes jaw deformities in Japanese flounder, Paralichthys olivaceus. Aquaculture 221, 381-392.
12. Haga, Y.; Dominique, V.; Du, S. J., 2009: Analyzing notochord segmentation and intervertebral disk formation using the twhh:gfp transgenic zebrafish model. Transgenic Res. 18, 669-683.
13. Hattori, M.; Sawada, Y.; Takagi, Y.; Suzuki, R.; Okada, T.; Kumai, H., 2003: Vertebral deformities in cultured red sea bream, Pagrus major, Temminck and Schlegel. Aquac. Res. 34, 1129-1137.
14. Inohaya, K.; Takano, Y.; Kudo, H., 2007: The teleost intervertebral region acts as a growth centre of the centrum: in vivo visualization of osteoblasts and their progenitors in transgenic fish. Dev. Dyn. 236, 3031-3046.
15. Ishioka, H.; Ueda, K.; Okamoto, R.; Fukuhara, O., 1970: The basic study on the production of marine fish seedling. Some characteristics of foreign bodies in the urinary bladder observed on marine fish, Pagrus major (Temminck et Schlegel). Bull. Nansei Reg. Fish. Res. Lab. 3, 11-17. [In Japanese with English Abstract].
16. Kawamura, K.; Hosoya, K., 1991: A modified double staining technique for making a transparent fish-skeletal specimen. Bull. Natl. Res. Inst. Aquac. 20, 11-18. [In Japanese with English Abstract].
17. Lall, S. P.; Lewis-McCrea, L. M., 2007: Role of nutrients in skeletal metabolism and pathology in fish - An overview. Aquaculture 267, 3-19.
18. Moretti, A.; Pedini Fernandez-Criado, M.; Cittolin, G.; Guidastri, R., 1999: Manual on hatchery production of seabass and gilthead sea bream, Vol. 1. FAO, Rome, Italy, 194 pp.
19. Moteki, M., 2002: Morphological aspects of feeding and improvement in feeding ability in the early larval stages of red sea bream Pagrus major. Fish. Sci. 68, 996-1003.
20. Nagano, N.; Hozawa, A.; Fujiki, W.; Yamada, T.; Miyaki, K.; Sakakura, Y.; Hagiwara, A., 2007: Skeletal development and deformities in cultured larval and juvenile seven-band grouper, Epinephelus septemfasciantus (Thunberg). Aquac. Res. 38, 121-130.
21. Nayler, R. L.; Hardy, R. W.; Bureau, D. P.; Chiu, A.; Elliot, M.; Farrell, A. P.; Foster, I.; Gatlin, D. M.; Goldburg, R. J.; Hua, K.; Nichols, P. D., 2009: Feeding aquaculture in an era of finite resources. Proc. Natl Acad. Sci. USA 106, 15103-15110.
22. Renn, J.; Winkler, C.; Schartl, M.; Fischer, R.; Goerlich, R., 2006: Zebrafish and medaka as models for bone research including implications regarding space-related issues. Protoplasma 229, 209-214.
23. Rufai, A.; Benjamin, M.; Ralphs, J. R., 1995: The development of fibrocartilage in the rat intervertebral disc. Anat. Embryol. 192, 53-62.
24. Sakai, Y.; Saito, S.; Shimizu, M.; Yamada, J.; Minato, I., 1996: Occurrence and properties of urinary calculi found in laboratoryraised larvae of Japanese flounder Paralichthys olivaceus. Nippon Suisan Gakkai Shi 62, 754-760.
25. Semba, K.; Araki, K.; Li, Z.; Matsumoto, K.; Suzuki, M.; Nakagata, N.; Takagi, K.; Takeya, M.; Yoshinobu, K.; Araki, M.; Imai, K.; Abe, K.; Yamamura, K., 2006: A novel murine gene, Sickle tail, linked to the Danforth's short tail locus, is required for normal development of the intervertebral disc. Genetics 172, 445-456.
26. Suzuki, T.; Haga, Y.; Takeuchi, T.; Uji, S.; Hashimoto, H.; Kurokawa, T., 2003: Differentiation of chondrocytes and scleroblasts during dorsal fin skeletogenesis in flounder larvae. Dev. Growth Differ. 45, 435-448.
27. Walker, M. B.; Kimmel, C. B., 2007: A two-color acid-free cartilage and bone stain for zebrafish larvae. Biotech. Histochem. 82, 23-28.
28. Yamamoto, T.; Teruya, K.; Hara, T.; Hokazono, H.; Kai, I.; Hashimoto, H.; Furuita, H.; Matsunari, H.; Mushiake, K., 2009: Nutritional evaluation of rotifers in rearing tanks without water exchange during seed production of amberjack Seriola dumerili. Fish. Sci. 75, 697-705.
29. Yamashita, K., 1966: Fish pediatrics. Bull. Kanagawa Pref. Mus. 17, 15-18. [In Japanese].