A high-throughput microplate assay for simultaneous colorimetric quantification of multiple enzyme activities in soil

Applied Soil Ecology

Volumn 45, Issue 3, 2010, Pages 315-318

  Popova, Inna E ^a   Deng, Shiping ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

High throughput; Microplate assay; Soil enzyme

Indexed keywords

EID: 77954142974     PISSN: 09291393     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.apsoil.2010.04.004     Document Type: Article

References (25)

1. Bonin H.L., Griffiths R.P., Caldwell B.A. Effects of storage on measurements of potential microbial activities in stream fine benthic organic matter. J. Microbiol. Methods 1999, 38:91-99.
2. Browman M.G., Tabatabai M.A. Phosphodiesterase activity of soils. Soil Sci. Soc. Am. J. 1978, 42:284-290.
3. DeForest J.L. The influence of time, storage temperature, and substrate age on potential soil enzyme activity in acidic forest soils using MUB-linked substrates and l-DOPA. Soil Biol. Biochem. 2009, 41:1180-1186.
4. Eivazi F., Tabatabai M.A. Phosphatases in soils. Soil Biol. Biochem. 1977, 9:167-172.
5. Eivazi F., Tabatabai M.A. Glucosidases and galactosidases in soils. Soil Biol. Biochem. 1988, 20:601-606.
6. Finzi A.C., Sinsabaugh R.L., Long T.M., Osgood M.P. Microbial community responses to atmospheric carbon dioxide enrichment in a warm-temperate forest. Ecosystems 2006, 9:215-226.
7. Freeman C., Liska G., Ostle N.J., Jones S.E., Lock M.A. The use of fluorogenic substrates for measuring enzyme-activity in peatlands. Plant Soil 1995, 175:147-152.
8. Gee G.W., Bauder J.W. Particle-size analysis by hydrometer-simplified method for routine textural analysis and a sensitivity test of measurement parameters. Soil Sci. Soc. Am. J. 1979, 43:1004-1007.
9. Hood M.A. Comparison of four methods for measuring chitinase activity and the application of the 4-MUF assay in aquatic environments. J. Microbiol. Methods 1991, 13:151-160.
10. Kremer R.J. Determination of soil phosphatase activity using a microplate method. Commun. Soil Sci. Plant Anal. 1994, 25:319-325.
11. Marx M.C., Wood M., Jarvis S.C. A microplate fluorimetric assay for the study of enzyme diversity in soils. Soil Biol. Biochem. 2001, 33:1633-1640.
12. Miller M., Palojarvi A., Rangger A., Reeslev M., Kjoller A. The use of fluorogenic substrates to measure fungal presence and activity in soil. Appl. Environ. Microbiol. 1998, 64:613-617.
13. Nakamura T., Mochida K., Ozoe Y., Ukawa S., Sakai M., Mitsugi S. Enzymological properties of three soil hydrolases and effects of several pesticides on their activities. J. Pestic. Sci. 1990, 15:593-598.
14. Naseby D.C., Lynch J.M. Enzymes and microorganisms in the rhizosphere. Enzymes in the Environment: Activity, Ecology, and Applications 2002, 109-123. CRC Press, London, UK. R.G. Burns, R.P. Dick (Eds.).
15. Nelson D.W., Sommers L.E. Total carbon, organic carbon, and organic matter. Part 3. Methods of Soil Analysis 1996, 961-1010. Agronomy Society of America and Soil Science Society of America, Madison, WI, USA. D.L. Sparks (Ed.).
16. Parham J.A., Deng S.P. Detection, quantification and characterization of beta-glucosaminidase activity in soil. Soil Biol. Biochem. 2000, 32:1183-1190.
17. Powlson D.S., Brookes P.C., Christensen B.T. Measurement of soil microbial biomass provides an early indication of changes in total soil organic-matter due to straw incorporation. Soil Biol. Biochem. 1987, 19:159-164.
18. Pritsch K., Raidl S., Marksteiner E., Blaschke H., Agerer R., Schloter M., Hartmann A. A rapid and highly sensitive method for measuring enzyme activities in single mycorrhizal tips using 4-methylumbelliferone-labelled fluorogenic substrates in a microplate system. J. Microbiol. Methods 2004, 58:233-241.
19. Saiya-Cork K.R., Sinsabaugh R.L., Zak D.R. The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol. Biochem. 2002, 34:1309-1315.
20. Sinsabaugh R.L., Reynolds H., Long T.M. Rapid assay for amidohydrolase (urease) activity in environmental samples. Soil Biol. Biochem. 2000, 32:2095-2097.
21. Tabatabai M.A. Soil enzymes. Methods of Soil Analysis. Part 2. Microbiological and Biochemical Properties. 1994, 775-833. Soil Science Society of America, Madison, WI, USA. R.W. Weaver, S. Angle, P. Bottomley, D. Bezdicek, S. Smith, A. Tabatabai, A. Wollum (Eds.).
22. Tabatabai M.A., Bremner J.M. Use of p-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1969, 1:301-307.
23. Tabatabai M.A., Bremner J.M. Arylsulfatase activity of soils. Soil Sci. Soc. Am. Proc. 1970, 34:225-229.
24. Wirth S.J., Wolf G.A. Micro-plate colourimetric assay for endo-acting cellulase, xylanase, chitinase, 1,3-[beta]-glucanase and amylase extracted from forest soil horizons. Soil Biol. Biochem 1992, 24:511-519.
25. Zornoza R., Landi L., Nannipieri P., Renella G. A protocol for the assay of arylesterase activity in soil. Soil Biol. Biochem. 2009, 41:659-662.