High-level soluble expression of hIGF-1 fusion protein in recombinant Escherichia coli

Process Biochemistry

Volumn 45, Issue 8, 2010, Pages 1401-1405

  Zhang, Danping ^a   Wei, Peilian ^b   Fan, Limei ^c   Lian, Jiazhang ^a   Huang, Lei ^a   Cai, Jin ^a   Xu, Zhinan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c ZHEJIANG UNIVERSITY CITY COLLEGE   (China)

Author keywords

E. coli Rosetta gami (DE3); HIGF 1; Insulin like growth factor; Soluble fusion expression; TrxA

Indexed keywords

CELL DISRUPTION; CONCENTRATION OF; DISULFIDE BONDS; E. COLI; EXPRESSION LEVELS; FUSION EXPRESSION; FUSION PROTEINS; GROWTH FACTOR; INSULIN-LIKE GROWTH FACTOR; OPTIMIZED CONDITIONS; RECOMBINANT ESCHERICHIA COLI; ROSETTA; SOLUBLE EXPRESSION; SOLUBLE PROTEINS; VOLUMETRIC PRODUCTION;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; INSULIN; PEPTIDES;

ESCHERICHIA COLI;

ESCHERICHIA COLI;

EID: 77954089776     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.05.014     Document Type: Article

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