Adaptation and validation of DNA synthesis detection by fluorescent dye derivatization for high-throughput screening

BioTechniques

Volumn 48, Issue 5, 2010, Pages 379-386

  Ranall, Max V ^a   Gabrielli, Brian G ^a   Gonda, Thomas J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

Author keywords

Cell based assay; Click; CuAAC; DNA synthesis; Ethynyl deoxyuridine; High content; High throughput; Proliferation; S phase

Indexed keywords

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; 2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 2 MORPHOLINO 8 PHENYLCHROMONE; 4 (3 CHLOROANILINO) 6,7 DIMETHOXYQUINAZOLINE; 5 ETHYNYLDEOXYURIDINE; CELL DNA; DEOXYURIDINE DERIVATIVE; FLUORESCENT DYE; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE B; UNCLASSIFIED DRUG; 4',6 DIAMIDINO 2 PHENYLINDOLE; 5-ETHYNYL-2'-DEOXYURIDINE; DEOXYURIDINE; DNA; EPIDERMAL GROWTH FACTOR; INDOLE DERIVATIVE; PROTEIN KINASE INHIBITOR;

ARTICLE; CELL PROLIFERATION; DERIVATIZATION; DNA CONTENT; DNA SYNTHESIS; HIGH THROUGHPUT SCREENING; IC 50; IMAGE ANALYSIS; NONHUMAN; SIGNAL TRANSDUCTION; ANALOGS AND DERIVATIVES; BIOSYNTHESIS; CELL LINE; CELL NUCLEUS; CHEMISTRY; DRUG EFFECTS; FLUORESCENCE MICROSCOPY; HUMAN; METABOLISM; PROCEDURES; REPRODUCIBILITY; VALIDATION STUDY;

CELL LINE; CELL NUCLEUS; DEOXYURIDINE; DNA; EPIDERMAL GROWTH FACTOR; FLUORESCENT DYES; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INDOLES; MICROSCOPY, FLUORESCENCE; PROTEIN KINASE INHIBITORS; REPRODUCIBILITY OF RESULTS;

EID: 77954077170     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113410     Document Type: Article

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