Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

BMC Genomics

Volumn 11, Issue 1, 2010, Pages

  Kapteyn, Jeremy ^a   He, Ruifeng ^a,b   McDowell, Eric T ^a   Gang, David R ^a,b  

^a UNIVERSITY OF ARIZONA   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; NUCLEOTIDE; OLIGONUCLEOTIDE; PLANT RNA; RNA; RNA DIRECTED DNA POLYMERASE;

5' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; DNA MODIFICATION; DNA SYNTHESIS; ISOMER; NONHUMAN; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; TEMPLATE SWITCHING POLYMERASE CHAIN REACTION; BIOSYNTHESIS; CHEMISTRY; CYTOLOGY; DNA SEQUENCE; ENZYMOLOGY; GENE LIBRARY; GENETICS; ISOMERISM; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLONEY LEUKEMIA ONCOVIRUS; NUCLEOTIDE SEQUENCE; PLANT;

BASE SEQUENCE; DNA, COMPLEMENTARY; GENE LIBRARY; ISOMERISM; MOLECULAR SEQUENCE DATA; MOLONEY MURINE LEUKEMIA VIRUS; OLIGONUCLEOTIDES; PLANTS; POLYMERASE CHAIN REACTION; RNA; RNA-DIRECTED DNA POLYMERASE; SEQUENCE ANALYSIS, DNA;

EID: 77954070555     PISSN: None     EISSN: 14712164     Source Type: Journal    
DOI: 10.1186/1471-2164-11-413     Document Type: Article

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