The HDR gene involved in the TIA pathway from Rauvolfia verticillata: Cloning, characterization and functional identification

Journal of Medicinal Plants Research

Volumn 4, Issue 10, 2010, Pages 915-924

  Chen, Jinwu ^a   Liu, Wanhong ^b   Chen, Min ^a   Wang, Guijun ^a   Peng, Meifang ^a   Chen, Rong ^a   Yang, Chunxian ^a   Lan, Xiaozhong ^c   Ming, Xingjia ^d   Hsieh, Minghsiun ^e   Liao, Zhihua ^a  

^a SOUTHWEST UNIVERSITY   (China)

^b CHONGQING UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c Tibet University   (Austria)

^d CHONGQING ACADEMY OF CHINESE MATERIA MEDICA   (China)

^e INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Cloning; Expression profile; Functional complementation; HDR gene; Rauvolfia verticillata

Indexed keywords

1 HYDROXY 2 METHYL 2 BUTENYL 4 DIPHOSPHATE REDUCTASE; ABSCISIC ACID; ACETYLSALICYLIC ACID; AMINO ACID DERIVATIVE; COMPLEMENTARY DNA; INDOLE ALKALOID; JASMONIC ACID METHYL ESTER; OXIDOREDUCTASE; POLYPEPTIDE; TERPENOID DERIVATIVE; UNCLASSIFIED DRUG;

APOCYNACEAE; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; FLOWER; FRUIT; GENE EXPRESSION PROFILING; GENE FUNCTION; GENE LOCATION; GENETIC CONSERVATION; GENETIC TRANSFORMATION; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHENOTYPE; PHYLOGENY; PLANT GENE; PLANT LEAF; PLANT ROOT; PLANT STEM; PLASTID; PROTEIN FAMILY; RAUVOLFIA VERTICILLATA; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION; ULTRAVIOLET RADIATION; UPREGULATION;

ARA; RAUVOLFIA; VERTICILLATA;

EID: 77954066936     PISSN: 19960875     EISSN: 19960875     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Altincicek B, Duin EC, Reichenberg A, Hedderich R, Kollas AK, Hintz M, Wagner S, Wiesner J, Beck E, Jomaa H (2002). LytB protein catalyzes the terminal step of the 2-C-methyl-D-erythritol-4-phosphate pathway of isoprenoid biosynthesis. FEBS Lett. 532: 437-440.
2. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
3. Anitha S, Ranjitha Kumari BD (2006). Stimulation of reserpine biosynthesis in the callus of Rauvolfia tetraphylla L. by precursor feeding. Afr. J. Biotechnol. 5: 659-661.
4. Botella-Pavía P, Besumbes Ó, Phillips MA, Carretero-Paulet L, Boronat A, Rodríguez-Concepción M (2004). Regulation of carotenoid biosynthesis in plants: evidence for a key role of hydroxymethylbutenyl diphosphate reductase in controlling the supply of plastidial isoprenoid precursors. Plant J. 40: 188-199.
5. Cunningham FX, Jr Lafond TP, Gantt E (2000). Evidence of a role for LytB in the nonmevalonate pathway of isoprenoid biosynthesis. J. Bacteriol. 182: 5841-5848.
6. Emanuelsson O, Nielsen H, Brunak S, Von Heijne G (2000). Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J. Mol. Biol. 300: 1005-1016.
7. Farooqi AA, Sreeramu BS (2001). Cultivation of Medicinal and Aromatic Crops. (Pub.) University Press Ltd., India pp. 210-211.
8. Gong YF, Liao ZH, Guo BH, Sun XF, Tang KX (2005). Molecular cloning and expression profile analysis of Ginkgo biloba DXS gene encoding 1-deoxy-d-xylulose 5-phosphate Synthase, the first committed enzyme of the 2-C-methyl-d-erythritol 4-phosphate pathway. Planta Medica 68: 1-7.
9. Guevara-Garcia A, San Roman C, Arroyo A, Cortes ME, M de la Luz Gutierrez-Nava, Leon P(2005). Characterization of the Arabidopsis clb6 mutant illustrates the importance of posttranscriptional regulation of the methyl-D-erythritol 4 phosphate pathway. Plant Cell 17: 628-643.
10. Hsieh MH, Goodman HM (2005). The arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis. Plant Physiol. 138: 641-653.
11. Kim SM, Kuzuyama T, Kobayashi A, Sando T, Chang YJ, Kim SU (2008). 1-Hydroxy-2-methyl-2-(E)-butenyl 4 diphosphate reductase (IDS) is encoded by multicopy genes in gymnosperms Ginkgo biloba and Pinus taeda. Planta 227: 1432-2048.
12. Kumar S, Tamura K, Nei M (2004). MEGA3: Integrated Software for Molecular Evolutionary Genetics Analysis and Sequence Alignment. Brie Bioinform 5: 150-163.
13. Lichtenthaler HK, Rohmer M, Schwender J (1997). Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. Physiol. Plant. 1016: 43-52.
14. Li HY, Ting KS (1962). Haemodynamic studies on the total alkaloids of Rauvolfia verticillata. Scientia Sinica 11: 791-804.
15. McAteer S, Coulson A, McLennan N, Masters M (2001). The lytB gene of Escherichia coli is essential and specifies a product needed for isoprenoid biosynthesis. J. Bacteriol. 183: 7403-7407.
16. Ramos-Valdivia AC, Van Der Heijden R, Verpoorte R (1998). Isopentenyl diphosphate isomerase and prenyltransferase activities in rubiaceous and apocynaceous cultures. Phytochemistry 48: 961- 949.
17. Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406-425.
18. Seemann M, Bui BT, Wolff M, Tritsch D, Campos N, Boronat A, Marquet A, Rohmer M (2002). Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2- enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein. Angew. Chem. Int. Ed. Engl. 41: 433-339.
19. Sheludo YU, Gerasimenko I, Unger M, Kostenyuk I, Stockigt J (1998). Induction of alkaloid diversity in hybrid plant cell lines. Plant Cell Rep. 18: 911-918.
20. Thompson LD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997). The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24: 4876-4882.
21. Wang Q, Pi Y, Hou R, Jiang K, Huang Z, Hsieh MS, Sun X, Tang K (2008). Molecular cloning and characterization of 1 hydroxy-2-methyl- 2-(E)-butenyl 4-diphosphate reductase (CaHDR) from Camptotheca acuminata and its functional identification in Escherichia coli. BMB Rep. 41: 112-118.
22. Wolff M, Seemann M, Tse Sum Bui B, Frapart Y, Tritsch D, Estrabot AG, Rodriguez-Concepcion M, Boronat A, Marquet A, Rohmer M (2003). Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut 2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein. FEBS Lett. 541: 115-120.
23. Yamazaki Y, Urano A, Sudo H, Kitajima M, Takayama H, Yamazaki M, Aimi N, Saito K (2003). Metabolite profiling of alkaloids and strictosidine synthase activity in camptothecin producing plants. Phytochemistry 62: 461-470.