Nuclear DNA content of Hydrastis canadensis L. and genome size stability of in vitro regenerated plantlets

Plant Cell, Tissue and Organ Culture

Volumn 102, Issue 2, 2010, Pages 259-263

  Obae, Samuel G ^a   West, Todd P ^a  

^a WEST VIRGINIA UNIVERSITY   (United States)

Author keywords

Flow cytometry; Germplasm conservation; Goldenseal; Medicinal plant; Tissue culture; Yellow root

Indexed keywords

GENOME SEQUENCING; GENOME SIZES; GERMPLASM CONSERVATION; GERMPLASMS; IN-VITRO; LONG-TERM CULTURE; MEDICINAL PLANT; MEDICINAL PLANTS; MICROPROPAGATION; NUCLEAR DNA CONTENT; PLANTING STOCKS; PLANTLETS;

CULTIVATION; DNA; FLOW CYTOMETRY; FORESTRY; GENE ENCODING; TISSUE CULTURE;

PLANTS (BOTANY);

HYDRASTIS; HYDRASTIS CANADENSIS;

EID: 77954034005     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-010-9719-3     Document Type: Article

References (22)

1. AHPA (2007) Tonnage survey of select North American wild-harvested plants, 2004-2005. American Herbal Products Association, Silver Spring, Maryland. Available via DIALOG. http://www. ahpa. org/Default. aspx?tabid=68#2004tonnagesurvey. Accessed on 18 Nov 2009.
2. Arumuganathan K, Earle ED (1991) Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Biol Rep 9: 229-241.
3. Bannerman J (1997) Goldenseal in world trade: pressures and potentials. HerbalGram 41: 51-52.
4. Bedir E, Lata H, Schaneberg B, Moraes RM (2004) In vitro plant regeneration from leaf-derived callus in H. canadensis (Hydrastis candensis). Acta Hort 629: 277-281.
5. Bennett MD, Leitch IJ (2005) Plant DNA C-values database release 4. 0. Royal Botanic Gardens, Kew. Availabbe via DIALOG. http://data. kew. org/cvalues. Accessed on 21 Oct 2009.
6. Bennett MD, Smith JB (1991) Nuclear DNA amounts in angiosperms. Philos Trans R Soc Lond B Biol Sci 334: 309-345.
7. Bennett MD, Leitch IJ, Price HJ, Johnston JS (2003) Comparisons with Caenorhabditis (~100 Mb) and Drosophila (~175 Mb) using flow cytometry showgenome size in Arabidopsis to be ~157 Mb and thus ~25% larger than the Arabidopsis genome initiative estimate of ~125 MB. Ann Bot 91: 547-557.
8. Blumenthal M (1999) Herb market levels after five years of bloom: 1999 sales in mainstream market up only 11% in first half of 1999 after 55% increase in 1998. HerbalGram 47: 64-65.
9. CITES (1997) Proposal for the inclusion of Hydrastis Canadensis. Appendix II, CITES.
10. Cole IB, Saxena PK, Murch SJ (2007) Medical biotechnology in the genus scutellaria. In Vitro Cell Dev Biol-Plant 43: 318-327.
11. Doležel J, Greilhuber J, Lucretti S, Meuster A, Lysák MA, Obermayer R (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparisons. Ann Bot 82(Suppl A): 17-26.
12. Doležel J, Bartoš J, Voglmayr H, Greilhuber J (2003) Nuclear DNA content and genome size of trout and human. Cytometry A 51: 127-128.
13. Doležel J, Greilhuber J, Suda J (2007) Estimation of nuclear DNA content in plants using flow cytometry. Nature Prot 2(9): 2233-2244.
14. Foster S, Duke J (2000) A field guide to medicinal plants and herbs of eastern and central North America, 2nd edn. Houghton Mifflin Company, New York, pp 57-58.
15. Gregory TR (2005) The C-value enigma in plants and animals: a review of parallels and an appeal for partnership. Ann Bot 95: 133-146.
16. Hall KC, Camper ND (2002) Tissue culture of goldenseal (Hydrastis canadensis L.). In Vitro Cell Dev Biol-Plant 38: 293-295.
17. He SS, Liu CZ, Saxena PK (2007) Plant regeneration of an endangered medicinal plant Hydrasis canadensis L. Scientia Hort 113: 82-86.
18. Liu CZ, Murch SJ, Jain JC, Saxena PK (2004) Goldenseal (Hydrastis canadensis L.): In vitro regeneration for germplasm and elimination of heavy metal contamination. In Vitro Cell Dev Biol-Plant 40: 75-79.
19. Price HJ, Hodnett G, Johnston JS (2000) Sunflower (Helianthus annuus) leaves contain compounds that reduce nuclear propidium iodide fluorescence. Ann Bot 86: 929-934.
20. SAS Institute, Inc (2003) SAS proprietary software version 9. 1. SAS Inst, Cary, NC.
21. Seazzocchio F, Cometa MF, Tomassini L, Palmery M (2001) Antibacterial activity of Hydrastis canadensis extract and its major isolated alkaloids. Planta Med 67: 561-564.
22. Sliwinska E, Thiem B (2007) Genome size stability in six medicinal plant species propagated in vitro. Biol Plant 51(3): 556-558.