A new technique for obtaining whole pathogen transcriptomes from infected host tissues

BioTechniques

Volumn 48, Issue 2, 2010, Pages 139-144

  Azhikina, Tatyana L ^a   Skvortsov, Timofey A ^a   Radaeva, Tatyana V ^b   Mardanov, Andrey V ^c   Ravin, Nikolay V ^c   Apt, Alexander S ^b   Sverdlov, Eugene D ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b CENTRAL TUBERCULOSIS RESEARCH INSTITUTE   (Russian Federation)

^c INSTITUTE OF BIOENGINEERING   (Russian Federation)

Author keywords

Coincidence cloning; Intracellular pathogen transcriptome; Mycobacterium tuberculosis

Indexed keywords

BACTERIAL DNA; COMPLEMENTARY DNA; GENOMIC DNA; RNA; TRANSCRIPTOME; BACTERIAL RNA;

AEROBIC METABOLISM; ANAEROBIC METABOLISM; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL GENOME; CELL RESPIRATION; CONTROLLED STUDY; DNA DENATURATION; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSCRIPTION; HIGH THROUGHPUT SCREENING; HOST; HOST RESISTANCE; IN VIVO STUDY; INTRACELLULAR BACTERIUM; LIPID METABOLISM; LUNG INFECTION; MOLECULAR CLONING; MOUSE; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; NUCLEIC ACID RENATURATION; ANIMAL; DNA SEQUENCE; GENE EXPRESSION PROFILING; GENETICS; HOST PATHOGEN INTERACTION; LUNG; METABOLISM; METHODOLOGY; MICROBIOLOGY; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; TUBERCULOSIS;

BACTERIA (MICROORGANISMS); MYCOBACTERIUM TUBERCULOSIS; PROTISTA;

ANIMALS; CLONING, MOLECULAR; DNA, BACTERIAL; DNA, COMPLEMENTARY; GENE EXPRESSION PROFILING; HOST-PATHOGEN INTERACTIONS; LUNG; MICE; MYCOBACTERIUM TUBERCULOSIS; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; SEQUENCE ANALYSIS, DNA; TUBERCULOSIS;

EID: 77953988966     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113350     Document Type: Article

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