Improved PCR flexibility with hot start dNTPs

BioTechniques

Volumn 47, Issue 4, 2009, Pages 881-882

  Le, Tony ^a   Paul, Natasha ^a  

^a TriLink BioTechnologies Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE TRIPHOSPHATE; DNA POLYMERASE; NUCLEOTIDE; TETRAHYDROFURAN; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; GENE AMPLIFICATION; MOLECULAR BIOLOGY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; SHORT SURVEY; TEMPERATURE;

EID: 77953984849     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113274     Document Type: Short Survey

References (4)

1. Chou, Q., Russell, M., Birch, D.E., Raymond, J. and Bloch, W. (1992) Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res, 20, 1717-1723.
2. Dang, C. and Jayasena, S.D. (1996) Oligonucleotide inhibitors of Taq DNA polymerase facilitate detection of low copy number targets by PCR. J Mol Biol, 264, 268-278. (Pubitemid 26402697)
3. Koukhareva, I. and Lebedev, A. (2009) 3′-protected 2′-deoxynucleoside 5′-triphosphates as a tool for heat-triggered activation of polymerase chain reaction. Anal Chem., 81, 4502-4509.
4. Ramakers, C., Ruijter, J.M., Deprez, R.H. and Moorman, A.F. (2003) Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data. Neurosci Lett, 339, 62-66. (Pubitemid 36263221)