Modified paraffin-embedding method for the human cochlea that reveals a fine morphology and excellent immunostaining results

Acta Oto-Laryngologica

Volumn 130, Issue 7, 2010, Pages 788-792

  Takahashi, Masatoki ^a   Kimura, Yurika ^b   Sawabe, Motoji ^c   Kitamura, Ken ^a  

^a TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^b Department of Otolaryngology ^*  (United States)

^c Tokyo Metropolitan Institute of Gerontology   (Japan)

Author keywords

Cochlea; Human temporal bone

Indexed keywords

ANTIBODY; EDETIC ACID; EOSIN; FORMALDEHYDE; HEMATOXYLIN; NEUROFIBRIL ANTIBODY; PARAFFIN; PRESTIN ANTIBODY; PYROXYLIN; UNCLASSIFIED DRUG;

ARTICLE; AUTOPSY; COCHLEA; CORTI ORGAN; EMBEDDING; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INNER EAR; MORPHOLOGY; PRIORITY JOURNAL; TEMPORAL BONE; TISSUE FIXATION; TISSUE SECTION;

COCHLEA; HUMANS; IMMUNOHISTOCHEMISTRY; PARAFFIN EMBEDDING; STAINING AND LABELING; TEMPORAL BONE;

EID: 77953945795     PISSN: 00016489     EISSN: 16512553     Source Type: Journal    
DOI: 10.3109/00016480903426626     Document Type: Article

References (13)

1. Schuknecht H. Temporal bone collections in Europe and the United States. Observations on a productive laboratory, pathologic findings of clinical relevance, and recommendations. Ann Otol Rhinol Laryngol Suppl 1987;130: 1-19.
2. Schuknecht H. Pathology of the ear, 2nd edn. Philadelphia: Lea and Febiger, 1993.
3. Merchant S, Burgess B, O'Malley J, Jones D, Adams J. Polyester wax: a new embedding medium for the histopathologic study of human temporal bones. Laryngoscope 2006;116:245-249
4. Merchant S, McKenna M, Adams J, Nadol JJ, Fayad J, Gellibolian R, et al. Human temporal bone consortium for research resource enhancement. Otol Neurotol 2008; 29:271-274
5. Kimura R. Animal models of inner ear vascular disturbances. Am J Otolaryngol 1982;7:130-139
6. Takahashi K, Merchant S, Miyazawa T, Yamaguchi T, McKenna M, Kouda H, et al. Temporal bone histopathological and quantitative analysis of mitochondrial DNA in MELAS. Laryngoscope 2003;113:1362-1368
7. O'Malley J, Merchant S, Burgess B, Jones D, Adams J. Effects of fixative and embedding medium on morphology and immunostaining of the cochlea. Audiol Neurootol 2009;14:78-87.
8. Keithley E, Horowitz S, Ruckenstein M. Na,K-ATPase in the cochlear lateral wall of human temporal bones with endolymphatic hydrops. Ann Otol Rhinol Laryngol 1995;104:858-863
9. Tian Q, Linthicum FJ, Keithley E. Application of labeling techniques to archival temporal bone sections. Ann Otol Rhinol Laryngol 1999;108:47-53.
10. Robertson N, Cremers C, Huygen P, Ikezono T, Krastins B, Kremer H, et al. Cochlin immunostaining of inner ear pathologic deposits and proteomic analysis in DFNA9 deafness and vestibular dysfunction. Hum Mol Genet 2006;15:1071-1085
11. O'Malley J, Burgess B, Jones D, Adams J, Merchant S. Techniques of celloidin removal from temporal bone sections. Ann Otol Rhinol Laryngol 2009;118:435-441
12. Kimura Y, Kouda H, Eishi Y, Kobayashi D, Suzuki Y, Ishige I, et al. Detection of mitochondrial DNA from human inner ear using real-time polymerase chain reaction and laser microdissection. Acta Otolaryngol 2005;125:697-701.
13. Kimura Y, Kubo S, Koda H, Noguchi Y, Sawabe M, Maruyama N, et al. Quantitative analysis of mRNA in human temporal bones. Acta Otolaryngol 2007;127: 1024-1030