Serum regulates adipogenesis of mesenchymal stem cells via MEK/ERK-dependent PPARγ expression and phosphorylation

Journal of Cellular and Molecular Medicine

Volumn 14, Issue 4, 2010, Pages 922-932

  Wu, Ling ^a,b   Cai, Xiaoxiao ^a   Dong, Hai ^c   Jing, Wei ^a   Huang, Yuanding ^a   Yang, Xingmei ^a   Wu, Yao ^a   Lin, Yunfeng ^a  

^a SICHUAN UNIVERSITY   (China)

^b UNIVERSITY OF TWENTE   (Netherlands)

^c TIBET UNIVERSITY   (China)

Author keywords

Adipogenesis; Extracellular signal regulated kinase; Mesenchymal stem cells; Mitogen activated protein kinase; Peroxisome proliferator activated receptor

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA; PROTEIN KINASE INHIBITOR;

ADIPOGENESIS; ANIMAL; ARTICLE; BAGG ALBINO MOUSE; CELL CULTURE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; MESENCHYMAL STEM CELL; METABOLISM; MOUSE; PHOSPHORYLATION; SERUM; SIGNAL TRANSDUCTION;

ADIPOGENESIS; ANIMALS; CELLS, CULTURED; ENZYME ACTIVATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GENE EXPRESSION REGULATION; MAP KINASE SIGNALING SYSTEM; MESENCHYMAL STEM CELLS; MICE; MICE, INBRED BALB C; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; PHOSPHORYLATION; PPAR GAMMA; PROTEIN KINASE INHIBITORS; SERUM;

EID: 77953935060     PISSN: 15821838     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1582-4934.2009.00709.x     Document Type: Article

References (50)

1. Turksen K. Adult stem cells 2004, 1st ed, Totowa
2. Le Blanc K, Pittenger M. Mesenchymal stem cells: progress toward promise. Cytotherapy 2005, 7:36-45.
3. Gronthos S, Zannettino AC, Hay SJ. Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci 2003, 116:1827-35.
4. Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med 2001, 226:507-20.
5. Cortesini R. Stem cells, tissue engineering and organogenesis in transplantation. Transpl Immunol 2005, 15:81-9.
6. Conrad C, Huss R. Adult stem cell lines in regenerative medicine and reconstructive surgery. J Surg Res 2005, 124:201-8.
7. Biddinger SB, Kahn CR. From mice to men: insights into the insulin resistance syndromes. Annu Rev Physiol 2006, 68:123-58.
8. Lehrke M, Lazar MA. The many faces of PPARgamma. Cell 2005, 123:993-9.
9. Kota BP, Huang TH, Roufogalis BD. An overview on biological mechanisms of PPARs. Pharmacol Res 2005, 51:85-94.
10. Fernyhough ME, Okine E, Hausman G. PPARgamma and GLUT-4 expression as developmental regulators/markers for preadipocyte differentiation into an adipocyte. Domest Anim Endocrinol 2007, 33:367-78.
11. Gurnell M. Peroxisome proliferator-activated receptor gamma and the regulation of adipocyte function: lessons from human genetic studies. Best Pract Res Clin Endocrinol Metab 2005, 19:501-23.
12. Kiec-Wilk B, Dembinska-Kiec A, Olszanecka A. The selected pathophysiological aspects of PPARs activation. J Physiol Pharmacol 2005, 56:149-62.
13. Chinetti G, Fruchart JC, Staels B. Peroxisome proliferator-activated receptors (PPARs): nuclear receptors at the crossroads between lipid metabolism and inflammation. Inflamm Res 2000, 49:497-505.
14. Fajas L, Auboeuf D, Raspe E. The organization, promoter analysis, and expression of the human PPARgamma gene. J Biol Chem 1997, 272:18779-89.
15. Rosen ED, Spiegelman BM. PPARgamma: a nuclear regulator of metabolism, differentiation, and cell growth. J Biol Chem 2001, 276:37731-4.
16. Knouff C, Auwerx J. Peroxisome proliferator-activated receptor-gamma calls for activation in moderation: lessons from genetics and pharmacology. Endocr Rev 2004, 25:899-918.
17. Kubota N, Terauchi Y, Miki H. PPAR gamma mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance. Mol Cell 1999, 4:597-609.
18. Rosen ED, Sarraf P, Troy AE. PPAR gamma is required for the differentiation of adipose tissue in vivo and in vitro. Mol Cell 1999, 4:611-7.
19. Barak Y, Nelson MC, Ong ES. PPAR gamma is required for placental, cardiac, and adipose tissue development. Mol Cell 1999, 4:585-95.
20. Hong JH, Hwang ES, McManus MT. TAZ, a transcriptional modulator of mesenchymal stem cell differentiation. Science 2005, 309:1074-8.
21. Camp HS, Tafuri SR, Leff T. c-Jun N-terminal kinase phosphorylates peroxisome proliferator-activated receptor-gamma1 and negatively regulates its transcriptional activity. Endocrinology 1999, 140:392-7.
22. Shao D, Rangwala SM, Bailey ST. Interdomain communication regulating ligand binding by PPAR-gamma. Nature 1998, 396:377-80.
23. Hu E, Kim JB, Sarraf P. Inhibition of adipogenesis through MAP kinase-mediated phosphorylation of PPARgamma. Science 1996, 274:2100-3.
24. Aouadi M, Laurent K, Prot M. Inhibition of p38MAPK increases adipogenesis from embryonic to adult stages. Diabetes 2006, 55:281-9.
25. Rochette-Egly C. Nuclear receptors: integration of multiple signalling pathways through phosphorylation. Cell Signal 2003, 15:355-66.
26. Zhang B, Berger J, Zhou G. Insulin- and mitogen-activated protein kinase-mediated phosphorylation and activation of peroxisome proliferator-activated receptor gamma. J Biol Chem 1996, 271:31771-4.
27. Bost F, Caron L, Marchetti I. Retinoic acid activation of the ERK pathway is required for embryonic stem cell commitment into the adipocyte lineage. Biochem J 2002, 361:621-7.
28. Machinal-Quelin F, Dieudonne MN, Leneveu MC. Proadipogenic effect of leptin on rat preadipocytes in vitro: activation of MAPK and STAT3 signaling pathways. Am J Physiol Cell Physiol 2002, 282:C853-63.
29. Prusty D, Park BH, Davis KE. Activation of MEK/ERK signaling promotes adipogenesis by enhancing peroxisome proliferator-activated receptor gamma (PPARgamma) and C/EBPalpha gene expression during the differentiation of 3T3-L1 preadipocytes. J Biol Chem 2002, 277:46226-32.
30. Lin YF, Jing W, Wu L. Identification of osteo-adipo progenitor cells in fat tissue. Cell Prolif 2008, 41:803-12.
31. Li ZY, Chen L, Liu L. Odontogenic potential of bone marrow mesenchymal stem cells. J Oral Maxillofac Surg 2007, 65:494-500.
32. Sen A, Lea-Currie YR, Sujkowska D. Adipogenic potential of human adipose derived stromal cells from multiple donors is heterogeneous. J Cell Biochem 2001, 81:312-9.
33. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25:402-8.
34. Prawitt J, Niemeier A, Kassem M. Characterization of lipid metabolism in insulin-sensitive adipocytes differentiated from immortalized human mesenchymal stem cells. Exp Cell Res 2008, 314:814-24.
35. Adams M, Reginato MJ, Shao DL. Transcriptional activation by peroxisome proliferator-activated receptor gamma is inhibited by phosphorylation at a consensus mitogen-activated protein kinase site. J Biol Chem 1997, 272:5128-32.
36. Bost F, Aouadi M, Caron L. The role of MAPKs in adipocyte differentiation and obesity. Biochimie 2005, 87:51-6.
37. Choi YS, Park SN, Suh H. Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres. Biomaterials 2005, 26:5855-63.
38. Guilak F, Lott KE, Award HA. Clonal analysis of the differentiation potential of human adipose-derived adult stem cells. J Cell Physiol 2006, 206:229-37.
39. Thuillier P, Baillie R, Sha X. Cytosolic and nuclear distribution of PPARgamma2 in differentiating 3T3-L1 preadipocytes. J Lipid Res 1998, 39:2329-38.
40. Moerman EJ, Teng K, Lipschitz DA. Aging activates adipogenic and suppresses osteogenic programs in mesenchymal marrow stroma/stem cells: the role of PPAR-gamma2 transcription factor and TGF-beta/BMP signaling pathways. Aging Cell 2004, 3:379-89.
41. Kersten S, Desvergne B, Wahli W. Roles of PPARs in health and disease. Nature 2000, 405:421-4.
42. Berger J, Patel HV, Woods J. A PPARgamma mutant serves as a dominant negative inhibitor of PPAR signaling and is localized in the nucleus. Mol Cell Endocrinol 2000, 162:57-67.
43. Kabashima T, Kawaguchi T, Wadzinski BE. Xylulose 5-phosphate mediates glucose-induced lipogenesis by xylulose 5-phosphate-activated protein phosphatase in rat liver. Proc Natl Acad Sci USA 2003, 100:5107-12.
44. Burgermeister E, Chuderland D, Hanoch T. Interaction with MEK causes nuclear export and downregulation of peroxisome proliferator-activated receptor gamma. Mol Cell Biol 2007, 27:803-17.
45. Janderova L, McNeil M, Murrell AN. Human mesenchymal stem cells as an in vitro model for human adipogenesis. Obes Res 2003, 11:65-74.
46. Hauner H, Loffler G. Adipogenic factors in human serum promote the adipose conversion of 3T3-L1 fibroblasts. Int J Obes 1986, 10:323-30.
47. Nixon T, Green H. Contribution of growth hormone to the adipogenic activity of serum. Endocrinology 1984, 114:527-32.
48. Richardson RL, Campion DR, Hausman GJ. Adhesion, proliferation, and adipogenesis in primary rat cell cultures: effects of collagenous substrata, fibronectin, and serum. Cell Tissue Res 1988, 251:123-8.
49. Oreffo RO, Virdi AS, Triffitt JT. Modulation of osteogenesis and adipogenesis by human serum in human bone marrow cultures. Eur J Cell Biol 1997, 74:251-61.
50. Ishizeki K, Takahashi N, Nawa T. Induction of adipogenesis by the intrasplenic transplantation of chick serum clots. Arch Histol Cytol 2004, 67:21-30.