A protocol for immunofluorescence staining of floating neurospheres

Neuroscience Letters

Volumn 479, Issue 2, 2010, Pages 126-127

  Sasaki, Ryo ^a   Aoki, Shunsuke ^b   Yamato, Masayuki ^a   Uchiyama, Hiroto ^a   Wada, Keiji ^c   Ogiuchi, Hideki ^a   Okano, Teruo ^a   Ando, Tomohiro ^a  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^b KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^c NATIONAL INSTITUTE OF NEUROSCIENCE   (Japan)

Author keywords

CD81 (TAPA1); Dental pulp cell; Immunofluorescence staining; Nestin; Neural stem cell; Neurosphere

Indexed keywords

CD81 ANTIGEN; NESTIN; BENZIMIDAZOLE DERIVATIVE; FLUORESCENT DYE; HOE 33342; INTERMEDIATE FILAMENT PROTEIN; LEUKOCYTE ANTIGEN; NERVE PROTEIN; NES PROTEIN, RAT;

ARTICLE; CENTRIFUGATION; CULTURE MEDIUM; FLUORESCENCE MICROSCOPY; FROZEN SECTION; IMMUNOFLUORESCENCE; NEURAL STEM CELL; NEUROSPHERE; PRECIPITATION; PRIORITY JOURNAL; TOOTH PULP; ANIMAL; CELL CULTURE; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; INNERVATION; METABOLISM; PROCEDURES; RAT; STAINING; STEM CELL;

ANIMALS; ANTIGENS, CD; BENZIMIDAZOLES; CELLS, CULTURED; DENTAL PULP; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; INTERMEDIATE FILAMENT PROTEINS; NERVE TISSUE PROTEINS; RATS; STAINING AND LABELING; STEM CELLS; ANTIGENS, CD81; NESTIN;

EID: 77953916251     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neulet.2010.05.042     Document Type: Article

References (11)

1. Campos L.S., Leone D.P., Relvas J.B., Brakebusch C., Fassler R., Suter U., Ffrench-Constant C. Beta1 integrins activate a MAPK signalling pathway in neural stem cells that contributes to their maintenance. Development 2004, 131:3433-3444.
2. Hall P.E., Lathia J.D., Miller N.G.A., Caldwell M.A., Ffrench-Constant C. Integrins are markers of human neural stem cells. Stem Cells 2006, 24:2078-2084.
3. Klassen H., Schwartz M.R., Bailey A.H., Young M.J. Surface markers expressed by multipotent human and mouse neural progenitor cells include tetraspanins and non-protein epitopes. Neurosci. Lett. 2001, 312:180-182.
4. Kopen G.C., Prockop D.J., Phinney D.G. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:10711-10726.
5. Lendahl U., Zimmerman L.B., McKay R.D. CNS stem cells express a new class of intermediate filament protein. Cell 1990, 60:585-595.
6. Moors M., Rockel T.D., Abel J., Cline J.E., Gassmann K., Schreiber T., Schuwald J., Weinmann N., Fritsche E. Human neurospheres as three-dimensional cellular systems for developmental neurotoxicity testing. Environ. Health Perspect. 2009, 117:1131-1138.
7. Reynolds B.A., Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 1992, 255:1707-1710.
8. Safford K.M., Hicok K.C., Safford S.D., Halvorsen Y.C., Wilkison W.O., Gimble J.M., Ricea H.E. Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem. Biophys. Res. Commun. 2002, 294:371-379.
9. Sasaki R., Aoki S., Yamato M., Uchiyama H., Wada K., Okano T., Ogiuchi H. Neurosphere generation from dental pulp of adult rat incisor. Eur. J. Neurosci. 2008, 27:538-548.
10. Toma J.G., Akhavan M., Fernandes K.J., Barnabe-Heider F., Sadikot A., Kaplan D.R., Miller F.D. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat. Cell Biol. 2001, 3:778-784.
11. Tropepe V., Coles B.L., Chiasson B.J., Horsford D.J., Elia A.J., McInnes R.R., van der Kooy D. Retinal stem cells in the adult mammalian eye. Science 2000, 287:2032-2036.