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Volumn 20, Issue 14, 2010, Pages 4259-4262

Screening and characterization of an inhibitory chemical specific to Arabidopsis gibberellin 2-oxidases

Author keywords

2 Oxoglutarate dependent dioxygenase (2ODD); Gibberellin; Gibberellin 2 oxidase; Inhibitor

Indexed keywords

2 OXOGLUTARIC ACID; 6 CHLORO 3H 1,2,3 BENZODITHIAZOLE 4 CARBOXYLATE 2 OXIDE; ENZYME INHIBITOR; GIBBERELLIN; GIBBERELLIN 2 OXIDASE; UNCLASSIFIED DRUG;

EID: 77953870026     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2010.05.015     Document Type: Article
Times cited : (8)

References (28)
  • 22
    • 77953873053 scopus 로고    scopus 로고
    • note
    • A coding region for a full-length GA2ox2 gene (Clone Name: U20502) was purchased from ABRC, and amplified by polymerase chain reaction with specific primers to its full-length cDNA sequences and suitable restriction enzyme sites (SacI and XhoI) at both ends of each primer. After a confirmation by sequence analyses, the cDNA fragment of GA2ox2 gene was ligated to pET32a vector (Novagen/Merck Biosciences, Madison, WI, USA). The preparation of recombinant GA2ox2 was confirmed on a SDS-PAGE profile, compared with a profile from negative control fraction (vector only). According to the manufacturer's procedure, affinity purification of GA2ox2 was performed by using its His-tag (Clontech TARON metal affinity resin, Takara Bio, Shiga, Japan).
  • 25
    • 77953872897 scopus 로고    scopus 로고
    • note
    • -1, He flow: 1.0 ml/min, ion source temperature: 280 °C, ionization: EI (70 eV)]. Each metabolite was identified by comparing its MS fragment pattern and Kovats' retention index (KRI) to those of authentic specimen.
  • 26
    • 77953872140 scopus 로고    scopus 로고
    • note
    • 3/EtOAc/AcOH (20:20:1, v/v/v). Radioactivity was visualized with an imaging plate (BAS-TR2040, Fuji Photo Film, Tokyo, Japan) and analyzer (FLA-5000, Fuji Photo Film). Quantification of metabolites and substrate that remained were estimated as pixels by scanning the imaging spots with CS Analyzer (Version 3.00.1010, ATTO, Tokyo).
  • 27
    • 77953873306 scopus 로고    scopus 로고
    • note
    • -1).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.