Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 23, 2010, Pages 10460-10465

  Brunnbauer, Melanie ^a,b   Mueller Planitz, Felix ^b,c   Kösem, Süleyman ^b,c   Ho, Thi Hieu ^b,c   Dombi, Renate ^b,c   Gebhardt, J Christof M ^a,b   Rief, Matthias ^a,b   Ökten, Zeynep ^b,c  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b Munich Center for Integrated Protein Science   (Germany)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

Molecular motors; Optical tweezers; Single molecule

Indexed keywords

HELMINTH PROTEIN; HETERODIMER; HOMODIMER; KINESIN 2; PROTEIN KLP11; PROTEIN KLP20; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; DIMERIZATION; ENZYME REGULATION; ENZYME SUBUNIT; EUKARYOTIC FLAGELLUM; MOTOR ACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROCESSING;

CAENORHABDITIS ELEGANS; EUKARYOTA;

EID: 77953762901     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1005177107     Document Type: Article

References (41)

1. Mehta AD, et al. (1999) Myosin-V is a processive actin-based motor. Nature 400:590-593.
2. Block SM, Goldstein LS, Schnapp BJ (1990) Bead movement by single kinesin molecules studied with optical tweezers. Nature 348:348-352.
3. Cross RA (2004) The kinetic mechanism of kinesin. Trends Biochem Sci 29:301-309.
4. Rock Ronald S, Purcell Thomas J, Spudich James A (2003) Mechanics of unconventional myosins. The Enzymes 23:55-86.
5. Cole DG (1999) Kinesin-II, the heteromeric kinesin. Cell Mol Life Sci 56:217-226.
6. Kozminski KG, Johnson KA, Forscher P, Rosenbaum JL (1993) A motility in the eukaryotic flagellum unrelated to flagellar beating. Proc Natl Acad Sci USA 90:5519-5523. (Pubitemid 23176192)
7. Scholey JM (2003) Intraflagellar transport. Annu Rev Cell Dev Biol 19:423-443.
8. Rosenbaum JL, Witman GB (2002) Intraflagellar transport. Nat Rev Mol Cell Biol 3:813-825.
9. Pan X, et al. (2006) Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. J Cell Biol 174:1035-1045. (Pubitemid 44455191)
10. Scholey JM (2008) Intraflagellar transport motors in cilia: Moving along the cell's antenna. J Cell Biol 180:23-29.
11. Snow JJ, et al. (2004) Two anterograde intraflagellar transport motors cooperate to build sensory cilia on C. elegans neurons. Nat Cell Biol 6:1109-1113.
12. Ou G, Blacque OE, Snow JJ, Leroux MR, Scholey JM (2005) Functional coordination of intraflagellar transport motors. Nature 436:583-587. (Pubitemid 41112934)
13. Yamazaki H, Nakata T, Okada Y, Hirokawa N (1996) Cloning and characterization of KAP3: a novel kinesin superfamily-associated protein of KIF3A/3B. Proc Natl Acad Sci USA 93:8443-8448.
14. Bisgrove BW, Yost HJ (2006) The roles of cilia in developmental disorders and disease. Development 133:4131-4143.
15. Pazour GJ, Rosenbaum JL (2002) Intraflagellar transport and cilia-dependent diseases. Trends Cell Biol 12:551-555.
16. Scholey JM (2003) Intraflagellar transport. Annu Rev Cell Dev Biol 19:423-443.
17. Mitchell DR (2007) The evolution of eukaryotic cilia and flagella as motile and sensory organelles. Adv Exp Med Biol 607:130-140.
18. Muthukrishnan G, Zhang Y, Shastry S, HancockWO (2009) The processivity of kinesin-2 motors suggests diminished front-head gating. Curr Biol 19:442-447.
19. Pierce DW, Hom-Booher N, Otsuka AJ, Vale RD (1999) Single-molecule behavior of monomeric and heteromeric kinesins. Biochemistry 38:5412-5421.
20. Imanishi M, Endres NF, Gennerich A, Vale RD (2006) Autoinhibition regulates the motility of the C. elegans intraflagellar transport motor OSM-3. J Cell Biol 174:931-937.
21. Hackney DD, Stock MF (2000) Kinesin's IAK tail domain inhibits initial microtubule-stimulated ADP release. Nat Cell Biol 2:257-260.
22. Hackney DD, Stock MF (2008) Kinesin tail domains and Mg2+ directly inhibit release of ADP from head domains in the absence of microtubules. Biochemistry 47:7770-7778.
23. Verhey KJ, Hammond JW (2009) Traffic control: Regulation of kinesin motors. Nat Rev Mol Cell Biol 10:765-777.
24. Dietrich KA, et al. (2008) The kinesin-1 motor protein is regulated by a direct interaction of its head and tail. Proc Natl Acad Sci USA 105:8938-8943.
25. Espeut J, et al. (2008) Phosphorylation relieves autoinhibition of the kinetochore motor Cenp-E. Mol Cell 29:637-643.
26. Hammond JW, et al. (2009) Mammalian Kinesin-3 motors are dimeric in vivo and move by processive motility upon release of autoinhibition. PLoS Biol 7:e72.
27. Friedman DS, Vale RD (1999) Single-molecule analysis of kinesin motility reveals regulation by the cargo-binding tail domain. Nat Cell Biol 1:293-297.
28. Jiang MY, Sheetz MP (1995) Cargo-activated ATPase activity of kinesin. Biophys J 68:283S-284S discussion 285S.
29. Hackney DD, Levitt JD, Suhan J (1992) Kinesin undergoes a 9 S to 6 S conformational transition. J Biol Chem 267:8696-8701.
30. Hirokawa N, et al. (1989) Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration. Cell 56:867-878.
31. Wedaman KP, Meyer DW, Rashid DJ, Cole DG, Scholey JM (1996) Sequence and submolecular localization of the 115-kD accessory subunit of the heterotrimeric kinesin-II (KRP85/95) complex. J Cell Biol 132:371-380. (Pubitemid 26052571)
32. Seiler S, et al. (2000) Cargo binding and regulatory sites in the tail of fungal conventional kinesin. Nat Cell Biol 2:333-338.
33. Coy DL, Hancock WO, Wagenbach M, Howard J (1999) Kinesin's tail domain is an inhibitory regulator of the motor domain. Nat Cell Biol 1:288-292. (Pubitemid 129495779)
34. Lupas A, Van Dyke M, Stock J (1991) Predicting coiled coils from protein sequences. Science 252:1162-1164. (Pubitemid 21917026)
35. Asbury CL, Fehr AN, Block SM (2003) Kinesin moves by an asymmetric hand-over-hand mechanism. Science 302:2130-2134.
36. Kaseda K, Higuchi H, Hirose K (2003) Alternate fast and slow stepping of a heterodimeric kinesin molecule. Nat Cell Biol 5:1079-1082. (Pubitemid 37509114)
37. Fehr AN, Gutierrez-Medina B, Asbury CL, Block SM (2009) On the origin of kinesin limping. Biophys J 97:1663-1670.
38. Yildiz A, TomishigeM, Vale RD, Selvin PR (2004) Kinesin walks hand-over-hand. Science 303:676-678.
39. Okten Z, Churchman LS, Rock RS, Spudich JA (2004) Myosin VI walks hand-over-hand along actin. Nat Struct Mol Biol 11:884-887. (Pubitemid 39128806)
40. Yildiz A, et al. (2003) Myosin V walks hand-over-hand: Single fluorophore imaging with 1.5-nm localization. Science 300:2061-2065. (Pubitemid 36760126)
41. Yildiz A, et al. (2004) Myosin VI steps via a hand-over-hand mechanism with its lever arm undergoing fluctuations when attached to actin. J Biol Chem 279:37223-37226. (Pubitemid 39195426)