Sulfated polysaccharides isolated from caulerpa racemosa and caulerpa cupressoides (chlorophyceaes) - extraction, fractionation and anticoagulant activity;Polissacarídeos sulfatados isolados das clorofíceas caulerpa racemosa e caulerpa cupressoides - extração, fracionamento e atividade anticoagulante

Acta Scientiarum - Biological Sciences

Volumn 32, Issue 2, 2010, Pages 113-120

  Rodrigues, José Ariévilo Gurgel ^a   Vanderlei, Edfranck de Sousa Oliveira ^b   Quinderé, Ana Luíza Gomes ^b   Fontes, Bruno Pedrosa ^b   Benevides, Norma Maria Barros ^b  

^a Rede Nordeste de Biotecnologia   (Brazil)

^b FEDERAL UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

APTT; Blood coagulation; Green algae; Sulfated macromolecules

Indexed keywords

ACETIC ACID; AGAROSE; BUFFER; DIETHYLAMINOETHYL CELLULOSE; HEPARIN; POLYSACCHARIDE SULFATE; SODIUM CHLORIDE;

ANTICOAGULATION; ARTICLE; CAULERPA; CAULERPA CUPRESSOIDES; CAULERPA RACEMOSA; DRUG ISOLATION; FRACTIONATION; GEL ELECTROPHORESIS; HUMAN; PARTIAL THROMBOPLASTIN TIME; SEAWEED;

ALGAE; CAULERPA CUPRESSOIDES; CAULERPA RACEMOSA; CHLOROPHYTA;

EID: 77953744205     PISSN: 16799283     EISSN: 1807863X     Source Type: Journal    
DOI: 10.4025/actascibiolsci.v32i2.5923     Document Type: Article

References (30)

1. ATHUKORALA, Y.; JUNG, W. K.; VASANTHAN, T.; JEON, Y. J. An anticoagulative polysaccharide from an enzymatic hydrolysate of Ecklonia cava. Carbohydrate Polymers, v. 66, n. 2, p.184-191, 2006.
2. ATHUKORALA, Y.; LEE, K. W.; KIM, S. K.; JEON, Y. J. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresource Technology, v. 98, n. 9, p. 1711-1716, 2007.
3. BARROSO, F. E. C.; RODRIDUES, J. A. G.; TORRES, V. M.; SAMPAIO, A. H.; FARIAS, W. R. L. Efeito do polissacarídeo sulfatado extraído da alga marinha vermelha Botryocladia occidentalis nas pós-larvas do camarão Litopenaeus vannamei. Revista Ciência Agronômica, v. 38, n. 1, p. 58-63, 2007.
4. BEZERRA-NETO, J. T. B.; RODRIGUES, J. A. G.; PONTES, G. C.; FARIAS, W. R. L. Polissacarídeos sulfatados da alga Caulerpa sertularioides (GMEL.) HOWE: análise de metodologias de precipitação. Revista Brasileira de Engenharia de Pesca, v. 3, n. 2, p. 50-62, 2008
5. BIRD, K. T. Agar production and quality from Gracilaria sp. strain G-16: effects of environmental factors. Botanica Marina, v. 31, n. 2, p. 33-39, 1988
6. BRADFORD, M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, v. 72, n. 1-2, p. 248-254, 1976
7. CAVATI, B.; FERNANDEZ, V. O. Algas perifíticas em dois ambientes do baixo rio Doce (lagoa Juparanã e rio Pequeno - Linhagens, Estado do Espírito Santo, Brasil: variação espacial e temporal. Acta Scientiarum. Biological Sciences, v. 30, n. 4, p. 439-448, 2008
8. CHOTIGEAT, W.; TONGSUPA, S.; SUPAMATAYA, K.; PHONGDARA, A. Effect of fucoidan on disease resistance of black tiger shrimp. Aquaculture, v. 233, n. 1-4, p. 23-30, 2004.
9. DIETRICH, C. P.; DIETRICH, S. M. C. Electrophoretic behaviour of acidic mucopolysaccharides in diamine buffers. Analytical Biochemistry, v. 70, n. 2, p. 645-647, 1976.
10. DODGSON, K. S.; PRICE, R. G. A note on the determination of the ester sulfato content of sulfated polysaccharides. Biochemical Journal, v. 84, n. 1, p. 106-110, 1962.
11. DUBOIS, M.; GILLES, K. A.; HAMILTON, J. K.; REBERS, P. A.; SMITH, F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, v. 28, n. 3, p. 350-356, 1956.
12. FARIAS, W. R. L.; VALENTE, A. P.; PEREIRA, M. S.; MOURÃO, P. A. S. Structure and anticoagulant activity of sulfated galactans. Isolation of a unique sulfated galactan from the red alga Botryocladia occidentalis and comparison of its anticoagulant action with that of sulfated galactans from invertebrates. Journal of Biological Chemistry, v. 275, n. 38, p. 29299-29307, 2000
13. FARNDALE, R. W.; BUTTLE, D. J.; BARRETT, A. J. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochimistry et Biophysica Acta, v. 883, n. 2, p. 173-177, 1986.
14. FONSECA, R. J. C.; OLIVEIRA, S. N. M. C. G.; MELO, F. R.; PEREIRA, M. G.; BENEVIDES, N. M. B.; MOURÃO, P. A. S. Slight differences in sulfatation of algal galactans account for differences in their anticoagulant and venous antithrombotic activities. Thrombosis and Haemostasis, v. 99, n. 3, p. 539-545, 2008.
15. GLICKSMAN, M. Food hydrocolloids: natural plant exudates - seaweed extracts. Baton Raton: CRC Press, 1983
16. MASUKO, T.; MINAMI, A.; IWASAKI, N.; MAJIMA, T.; NISHIMURA, S. I.; LEE, Y. C. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry, v. 339, n. 1, p. 69-72, 2005
17. MATSUBARA, K.; MATSUBARA, Y.; BASIC, A.; LIAO, M. L.; HORI, K.; MIYAZAWA, K. Anticoagulant properties of a sulfated galactan preparation from a marine green alga, Codium cylindricum. International Journal of Biological Macromolecules, v. 28, n. 5, p. 395-399, 2001
18. MOURÃO, A. P. S. Use of sulfated fucans as anticoagulant and antithrombotic agents: future perspectives. Current Pharmceutical Design, v. 10, n. 9, p. 967-981, 2004.
19. MOURÃO, P. A. S.; PEREIRA, M. S. Searching for alternatives to heparin: sulfated fucans from marine invertebrates. Trends Cardiovascular Medicine, v. 9, n. 8, p. 225-232, 1999.
20. NADER, H. B.; PINHAL, M. A. S.; BAÚ, E. C.; CASTRO, R. A. B.; MEDEIROS, G. F.; CHAVANTE, S. F.; LEITE, E. L.; TRINDADE, E. S.; SHINJO, S. K.; ROCHA, H. A. O.; TERSARIOL, I. L. S.; MENDES, A.; DIETRICH, C. P. Development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells. Brazilian Journal of Medical and Biological Research, v. 34, n. 6, p. 699-709, 2001.
21. PERCIVAL, E.; MCDOWELL, R. H. Chemistry and enzymology of marine algal polysaccharides. New York: Academic Press, 1967.
22. PEREIRA, M. G.; BENEVIDES, N. M. B.; MELO, M. R. S.; VALENTE, A. P.; MELO, F. R.; MOURÃO, P. A. S. Structure and anticoagulant activity of a sulfated galactan from the red alga, Gelidium crinale. Is there a specific structural requirement for the anticoagulant action? Carbohydrate Research, v. 340, n. 12, p. 2015-2023, 2005.
23. RODRIGUES, J. A. G.; FARIAS, W. R. L. Avaliação comparativa dos polissacarídeos sulfatados extraídos de rodofíceas do gênero Halymenia spp. Ferramenta taxonômica para algas? Revista Brasileira de Engenharia de Pesca, v. 4, n. 1, p. 7-20, 2009.
24. RODRIGUES, J. A. G.; TORRES, V. M.; ALENCAR, D. B.; SAMPAIO, A. H.; FARIAS, W. R. L. Extração e atividade anticoagulante dos polissacarídeos sulfatados da alga marinha vermelha Halymenia pseudofloresia. Revista Ciência Agronômica, v. 40, n. 2, p. 224-231, 2009.
25. SOUZA, M. L. S.; DELLIAS, J. M. M.; MELO, F. R. M.; SILVA, L. C. Structural composition and anticoagulant activity of dermatan sulfate from the skin of the electric eel, Electrophorus electricus (L). Comparative Biochemistry and Physiology. Parte B. Biochemistry and Molecular Biology, v. 147, n. 3, p. 387-394, 2007.
26. SZE, P. A biology to the algae. New York: McGraw- Hill, 1997.
27. TEIXEIRA, V. L. Produtos naturais marinhos. In: PEREIRA, R. C.; SOARES-GOMES, A. (Ed.). Biologia marinha. Rio de Janeiro: Interciência, 2002, p. 249-279
28. TORRES, M. R.; SOUSA, A. P. A.; FILHO, E. A. T. S.; MELO, D. F.; FEITOSA, J. P. A.; PAULA, R. C. M.; LIMA, M. G. S. Extraction and physicochemical characterization of Sargassum vugare alginate from Brazil. Carbohydrate Research, v. 342, n. 14, p. 2067-2074, 2007.
29. WEITZ, J. New anticoagulant strategies. Current status and future potentials. Drugs, v. 48, n. 1, p. 485-497, 1994
30. ZHANG, H. J.; MAO, W. J.; FANG, F.; LI, H. Y.; SUN, H. H.; CHEN, Y.; QI, X. H. Chemical characteristics and anticoagulant activities of a sulfated polysaccharide and its fragments from Monostroma latissimum. Carbohydrate Polymers, v. 71, n. 3, p. 428-434, 2008.