Optimization and analysis of a quantitative real-time PCR-based technique to determine microRNA expression in formalin-fixed paraffin-embedded samples

BMC Biotechnology

Volumn 10, Issue , 2010, Pages

  Goswami, Rashmi S ^a,b   Waldron, Levi ^a,c   Machado, Jerry ^a,b   Cervigne, Nilva K ^a   Xu, Wei ^a   Reis, Patricia P ^a   Bailey, Denis J ^b,d   Jurisica, Igor ^a,b,e   Crump, Michael R ^a   Kamel Reid, Suzanne ^a,b,d  

^a PRINCESS MARGARET HOSPITAL   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c UNIVERSITY HEALTH NETWORK   (Canada)

^d TORONTO GENERAL HOSPITAL   (Canada)

^e UNIVERSITY OF TORONTO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CORRELATION COEFFICIENT; DATA ANALYSIS; EXPRESSION ANALYSIS; EXPRESSION PROFILE; FORMALIN; FORMALIN FIXATION; FROZEN SAMPLE; HIGH REPRODUCIBILITY; HIGH-THROUGHPUT; HUMAN MICRO-RNA; MICRORNA EXPRESSION; MICRORNAS; NON-CODING RNAS; OPTIMAL PERFORMANCE; POST-TRANSCRIPTIONAL REGULATIONS; QUANTITATIVE PCR; QUANTITATIVE REAL TIME PCR; REPRODUCIBILITIES; RNA EXTRACTION; TISSUE DEVELOPMENT;

CELL DEATH; CELL PROLIFERATION; DATA REDUCTION; OPTIMIZATION; PARAFFIN WAXES; PARAFFINS; RNA;

FORMALDEHYDE;

MICRORNA; FORMALDEHYDE; PARAFFIN;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CORRELATION COEFFICIENT; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN TISSUE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RNA EXTRACTION; TRANSCRIPTION REGULATION; COMPARATIVE STUDY; METHODOLOGY; MICROFLUIDICS; POLYMERASE CHAIN REACTION; REGRESSION ANALYSIS;

FORMALDEHYDE; GENE EXPRESSION PROFILING; HUMANS; MICROFLUIDICS; MICRORNAS; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; REGRESSION ANALYSIS; REPRODUCIBILITY OF RESULTS;

EID: 77953689943     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-10-47     Document Type: Article

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