Proteome from lemon fruit flavedo reveals that this tissue produces high amounts of the cit s1 germin-like isoforms

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 12, 2010, Pages 7239-7244

  Pignataro, Vittorio ^a   Canton, Cristina ^b   Spadafora, Antonia ^a   Mazzuca, Silvia ^a  

^a UNIVERSITY OF CALABRIA   (Italy)

^b Ion Source and Biotechnologies   (Italy)

Author keywords

Cit s1; Germin like protein; Lemon flavedo; Plant allergens; Proteome

Indexed keywords

GLYCOPROTEIN; ISOPROTEIN; PROTEOME; VEGETABLE PROTEIN; GERMIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CITRUS FRUIT; FRUIT; GENETICS; GERMIN; METABOLISM; MOLECULAR GENETICS; PROTEOMICS; TWO DIMENSIONAL GEL ELECTROPHORESIS; CITRUS;

AMINO ACID SEQUENCE; CITRUS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FRUIT; GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; PROTEIN ISOFORMS; PROTEOME; PROTEOMICS;

CITRUS; CITRUS LIMON; CITRUS SINENSIS;

EID: 77953643849     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf1006825     Document Type: Article

References (30)

1. Spiegel-Roy, P. Goldschmidt, E. E. Biology of Citrus, 1st ed.; Cambridge University Press: Cambridge, U.K., 1996; pp 230.
2. Ahrazem, O.; Ibanez, M. D.; Lopez-Torrejon, G.; Sanchez-Monge, R.; Sastre, J.; Lombardero, M.; Barber, D.; Salcedo, G. Orange germin-like glycoprotein Cit s1: an equivocal allergen Int. Arch. Allergy Immunol. 2006, 139, 96-103
3. Lopez-Torrejon, G.; Ibanez, M. D.; Ahrazem, O.; Sanchez-Monge, R.; Sastre, J.; Lombardero, M.; Barber, D.; Salcedo, G. Isolation, cloning and allergenic reactivity of natural profilin Cit s2, a major orange allergen Allergy 2005, 60, 1424-1429
4. Ahrazem, O.; Ibanez, M. D.; Lopez-Torrejon, G.; Sanchez-Monge, R.; Sastre, J.; Lombardero, M.; Barber, D.; Salcedo, G. Lipid transfer proteins and allergy to orange Int. Arch. Allergy Immunol. 2005, 137, 201-210
5. Ibanez, M. D.; Sastre, J.; San Ireneo, M. M.; Laso, M. T.; Barber, D.; Lombardero, M. Different patterns of allergen recognition in children allergic to orange J. Allergy Clin. Immunol. 2004, 113, 175-177
6. Crespo, J. F.; Retzek, M.; Foetisch, K.; Sierra-Maestro, E.; Cid-Sanchez, A. B.; Pascual, C. Y.; Conti, A.; Feliu, A.; Rodriguez, J.; Vieths, S. Germin-like protein Cit s1 and profilin Cit s2 are major allergens in orange (Citrus sinensis) fruits Mol. Nutr. Food Res. 2006, 50, 282-290
7. Pöltl, G.; Ahrazem, O.; Paschinger, K.; Ibanez, M. D.; Salcedo, G.; Wilson, I. B. H. Molecular and immunological characterization of the glycosylated orange allergen Cit s1 Glycobiology 2007, 17, 220-230
8. Bernier, F.; Berna, A. Germins and germin-like proteins: plant do all proteins. But what do they do exactly? Plant Physiol. Biochem. 2001, 39, 545-554
9. Manosalva, P. M.; Davidson, R. M.; Liu, B.; Zhu, X.; Hulbert, S. H.; Hei, L.; Leach, J. E. A. Germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice Plant Physiol. 2009, 149, 286-296
10. Dunwell, J. M.; Gibbings, J. G.; Mahmood, T.; Naqvi, S. M. S. Germin and germin-like proteins: evolution, structure, and function Crit. Rev. Plant Sci. 2008, 27, 342-375
11. Zimmermann, G.; Baumlein, H.; Mock, H.; Himmelbach, A.; Schweizer, P. The multigene family encoding germin-like proteins of barley: regulation and function in basal host resistance Plant Physiol. 2006, 142, 181-192
12. Gucciardo, S.; Wisniewski, J. P.; Brewin, N. J.; Bornemann, S. A germin-like protein with superoxide dismutase activity in pea nodules with high protein sequence identity to a putative rhicadhesin receptor J. Exp. Bot. 2007, 58, 1161-1171
13. Carter, C.; Thornburg, R. W. Tobacco nectarin I. Purification and characterization as a germin-like, manganese superoxide dismutase implicated in the defense of floral reproductive tissues J. Biol. Chem. 2000, 275, 36726-36733
14. Heazlewood, J. L.; Millar, H. AMPDB: the Arabidopsis mitochondrial protein database Nucleic Acids Res. 2005, 33, 605-610
15. Ferro, M.; Brugière, S.; Salvi, D.; Seigneurin-Berny, D.; Court, M.; Moyet, L.; Ramus, C.; Miras, S.; Mellal, M.; Le Gall, S.; Kieffer-Jaquinod, S.; Bruley, C.; Garin, J.; Joyard, J.; Masselon, C.; Rolland, N. AT-CHLORO: A comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins. Mol. Cell Proteomics. 2010, doi: 10.1074/mcp.M900325-MCP200.
16. Maltman, D. J.; Gadd, S. M.; Simon, W. J.; Slabas, A. R. Differential proteomic analysis of the endoplasmic reticulum from developing and germinating seeds of castor (Ricinus communis) identifies seed protein precursor as significant component of the endoplasmic reticulum Proteomics 2007, 7, 1513-1528
17. Fukao, Y.; Hayashi, M.; Nishimura, M. Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana Plant Cell Physiol. 2002, 43, 689-696
18. Grennan, A. K. Plastoglobule proteome Plant Physiol. 2008, 147, 443-445
19. Katz, E.; Fon, M.; Lee, Y. J.; Phinney, B. S.; Sadka, A.; Blumwald, E. The citrus fruit proteome: insights into citrus fruit metabolism Planta 2007, 226, 989-1005
20. Lliso, I.; Tadeo, F. R.; Phinney, B. S.; Wilkerson, G. C.; Talo'n, M. Protein changes in the albedo of Citrus fruits on postharvesting storage J. Agric. Food Chem. 2007, 55, 9047-9053
21. Alos, E.; Roca, M.; Iglesias, D. J.; Minguez-Mosquera, M. I.; Borges Damasceno, C. M.; Thannhauser, T. W.; Campbell Rose, J. K.; Talon, M.; Cercos, M. An evaluation of the basis and consequences of a stay-green mutation in the navel negra citrus mutant using transcriptomic and proteomic profiling and metabolite analysis Plant Physiology 2008, 147, 1300-1131
22. Pignataro, V. Proteins characterization in lemon fruit (Citrus limon, L. Burm. f. var. Femminello, Rocca Imperiale). Ph.D. Thesis, XVII cycle School of Life Science, Doctorate course in Plant Biology, 2009.
23. Spadafora, A.; Filadoro, D.; Mazzuca, S.; Bracale, M.; Marsoni, M.; Cardilio, M.; Innocenti, A. M. 2-DE polypeptide mapping of Posidonia oceanica leaf, a molecular tool for marine environment analyses Plant Biosyst. 2008, 142, 213-218
24. Saravanan, R. S.; Rose, J. K. C. A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues Proteomics 2004, 4, 2522-2532
25. Wang, W.; Scali, M.; Vignani, R.; Spadafora, A.; Sensi, E.; Mazzuca, S.; Cresti, M. Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds Electrophoresis 2003, 24, 2369-2375
26. Shevchenko, A.; Wilm, M.; Vorm, O.; Mann, M. Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels Anal. Chem. 1996, 68, 850-858
27. Frank, A.; Pevzner, P. PepNovo: de novo peptide sequencing via probabilistic network modeling. Anal. Chem. 2005, 77 (4), 964 -973.
28. Lane, B. G. Oxalate, germin, and higher-plant pathogens IUBMB Life 2002, 53, 67-75
29. 2-producing enzyme, accumulates in barley attacked by the powdery mildew fungus Plant J. 1995, 8, 139-145
30. Hee, J. K.; Pesacreta, T. C.; Triplett, B. A. Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis Planta 2004, 218, 525-535