Comparison of normalization methods for construction of large, multiplex amplicon pools for next-generation sequencing

Applied and Environmental Microbiology

Volumn 76, Issue 12, 2010, Pages 3863-3868

  Harris, J Kirk ^a,e   Sahl, Jason W ^b   Castoe, Todd A ^c,e   Wagner, Brandie D ^d   Pollock, David D ^c,e   Spear, John R ^b  

^a Children's Hospital Colorado   (United States)

^b Department of Geology and Geological Engineering   (United States)

^c UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^d Global Health and Health Disparities   (United States)

^e UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPLICONS; COST SAVING; CRITICAL STEPS; EQUIMOLAR AMOUNT; GENETIC VARIATION; MANAGEMENT COSTS; MASSIVELY PARALLEL SEQUENCING; MICROBIAL COMMUNITIES; MICROBIAL ECOLOGY; MIXED SAMPLES; NORMALIZATION METHODS; NORMALIZATION PROCESS; QUANTITATIVE BINDING; RESEARCH QUESTIONS; SEQUENCE ACQUISITION; SEQUENCE DATA; SEQUENCE DIVERSITY; TEMPORAL AND SPATIAL VARIATION;

GENES; MULTIPLEXING;

LAKES;

COST-BENEFIT ANALYSIS; GENERATION TIME; GENETIC ANALYSIS; GENETIC VARIATION; MICROBIAL COMMUNITY; QUANTITATIVE ANALYSIS; SPATIOTEMPORAL ANALYSIS; SPECTROSCOPY;

ARTICLE; COMPARATIVE STUDY; DNA SEQUENCE; EVALUATION; GENE LIBRARY; METAGENOMICS; METHODOLOGY; SENSITIVITY AND SPECIFICITY; STAINING; EVALUATION STUDY; PROCEDURES;

GENE LIBRARY; METAGENOMICS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; STAINING AND LABELING;

EID: 77953624440     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02585-09     Document Type: Article

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